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Application of aryl naphthalene type lignan in preparation of medicine for resisting prostate cancer

An anti-prostate cancer, prostate cancer technology, applied in the field of medicine, to achieve strong pharmacological effects, inhibit proliferation, and good anti-prostate cancer activity

Inactive Publication Date: 2014-06-18
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] So far no arylnaphthyl lignan 6-hydroxy-4β-(4-hydroxy-3-methoxyphenyl)-3α-hydroxymethyl -7-Methoxy-3, 4-dihydro-2-naphthaldehyde in the treatment of prostate cancer report

Method used

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  • Application of aryl naphthalene type lignan in preparation of medicine for resisting prostate cancer
  • Application of aryl naphthalene type lignan in preparation of medicine for resisting prostate cancer
  • Application of aryl naphthalene type lignan in preparation of medicine for resisting prostate cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1: the preparation of aryl naphthalene type lignans

[0026] (1) Preparation of extraction solution: After crushing the medicinal materials of Vitex pratense, put them into the extraction tank, heat extraction with 40-90% ethanol for 2-3 times, the amount of solvent used each time is about 8-10 times the amount of crude drug, each extraction The time is 1 to 2 hours, and the extracts are combined;

[0027] (2) Refining, concentrating and drying: Concentrate the above extract under reduced pressure, recover the solvent, degrease the concentrated solution with petroleum ether, extract with ethyl acetate, and concentrate to obtain the ethyl acetate part;

[0028] (3) Separation and purification: Dissolve the above ethyl acetate part in water and load the macroporous adsorption resin AB-8. After the sample is adsorbed, wash with 5 times the volume of the column bed to remove impurities, and then sequentially use a volume fraction of 20 , 40, 60, 80, and 95% et...

Embodiment 2

[0030] Example 2: Arylnaphthyl Lignans on Human Prostate Cancer Cells (PC-3 (hormone-independent prostate cancer cell line), 22RV1 (hormone-dependent prostate cancer cell line) and C4-2 (hormone-independent prostate cancer cell line)) proliferation effects

[0031] (1) Cell culture

[0032] The PC-3, 22RV1 and C4-2 cell culture system was RPMI1640 medium (Gibco), containing 10% fetal bovine serum (hyclone), 100 U / ml penicillin, 100 U / ml streptomycin at 37°C, 5% CO 2 cultured under saturated humidity conditions.

[0033] (2) Cell proliferation test

[0034] Using the Cell Counting Kit-8 kit (CCK-8), a certain number of PC-3, 22RV1 and C4-2 cells in the exponential growth phase were inoculated on a 96-well plate, and divided into blank control group and administration (the drug is 6-Hydroxy-4β-(4-hydroxy-3-methoxyphenyl)-3α-hydroxymethyl-7-methoxy-3,4-dihydro-2-naphthylaldehyde) group (0.1,1 and 10 μM) 6 replicate wells were set up for each group. The cells were incubated...

Embodiment 3

[0036] Example 3: Effects of Arylnaphthyl Lignans on Invasion and Migration of Human Prostate Cancer Cells (PC-3 and C4-2)

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Abstract

The invention relates to an application of aryl naphthalene type lignan in preparing a medicine for resisting the prostate cancer. The aryl naphthalene type lignan is a compound as follows: 6-hydroxyl-4beta-(4-hydroxyl-3-methoxyphenyl)-3alpha-hydroxymethyl-7-methoxyl-3, 4-dihydro-2-naphthaldehyde. The invention also provides an extraction method of aryl naphthalene type lignan compounds. The application has the advantages that by in-vitro test of prostate cancer cells of a human on the compound, the aryl naphthalene type lignan can inhibit proliferation, invasion and migration of prostate cancer cells with different types, and has good activity for resisting the prostate cancer, so that the aryl naphthalene type lignan can be used for preparing the medicine for resisting the prostate cancer.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to an arylnaphthalene-type lignan compound 6-hydroxyl-4β-(4-hydroxyl-3-methoxyphenyl)-3α-hydroxymethyl-7-methoxy Application of base-3,4-dihydro-2-naphthaldehyde in the preparation of anti-prostate cancer drugs. Background technique [0002] Prostate cancer is one of the most common malignant tumors of the male genitourinary system, with high morbidity and mortality, and a serious threat to human health (Ahmedin Jemal, Rebecca Siegel, Jiaquan Xu and Elizabeth Ward. Cancer statistics, 2010. CA Cancer J Clin 2010, 60:277-300.). Antiandrogen therapy is one of the effective treatments for advanced prostate cancer, but after a period of time, almost all patients will continue to progress and transform into castration-resistant prostate cancer (CRPC), with a poor prognosis. CRPC patients rarely survive beyond 12 months (Thompson I, Thrasher JB, Aus G, et al. Guideline for the managemen...

Claims

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Application Information

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IPC IPC(8): A61K31/11A61P35/00C07C47/47C07C45/78
Inventor 孙颖浩郑承剑任善成秦路平许传亮
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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