Gene sequencing method

Abstract

The invention discloses a gene sequencing method. The gene sequencing method comprises the step of exciting three fluorescently-labeled nucleotide molecules by utilizing three different wavelengths of laser, wherein the nucleotide molecules are any three of A, C, G and T nucleotide molecules, the fourth nucleotide molecule has fluorescent nucleotide label. A sequencer is defined again by taking non-fluorescence excitation as a label through the method, so that the method is a method for sequencing synthesized sides by adopting tricolor fluorescent sides. The synthetic steps of the method are simplified, and an imaging device is simple, and low in cost; only three fluorescently-labeled nucleotide molecules are adopted, each sequencing period cannot leave excessive residual molecules on a DNA (deoxyribonucleic acid) chain, the combination of DNA polymerase and to-be-detected DNA chain cannot be influenced, and the sequencing accuracy and reading length can be greatly improved.

Description

technical field [0001] The invention belongs to the technical field of gene sequencing, and in particular relates to a gene sequencing method. Background technique [0002] DNA sequencing (DNA sequencing) refers to the analysis of the base sequence of a specific DNA fragment, that is, the arrangement of adenine (A), thymine (T), cytosine (C) and guanine (G). Fast, accurate and inexpensive DNA sequencing methods will greatly advance research and discovery in biology and medicine. It is now possible to sequence the entire genome, including the human genome and many other animal, plant and microbial species, contributing to a deeper understanding of the nature of life. Clinically, many disease-related genetic tests allow medical staff to better diagnose and treat patients, gradually reflecting the huge application prospects of gene sequencing. [0003] As early as 1977, Sanger et al. invented the dideoxy chain terminal termination method. At the same time, Maxam and Gilbert ...

AI Technical Summary

Problems solved by technology

[0006] However, the existing gene sequencing technology has the following problems: (1) Roche’s method has poor accuracy for DNA sequencing of the same sequence, because its method does not use synthesis termination technology, and requires the use of multiple enzymes, which is low in efficiency and high in cost , has been gradually eliminated by the market; (2) The disadvantage of Life Tech's DNA ligation sequencing is that it uses ligase instead of polymerase, so the accuracy is not good, and the cost is high; Better yet, its main disadvantage is the use of four fluorescent molecules, high cost, complex device, and each sequencing cycle will leave redundant residual molecules on the DNA chain

Images