Application of the refined liquid of Trichosporum fermented liquid as a bacteriostatic agent against Paecilomyces infecting bat moth larvae
A technology of Trichoderma spp. and bat moth larvae in China, which is applied in the fields of fermentation, antifungal agents, and medical preparations containing active ingredients, which can solve the problem of not clearly specifying the mechanism of action and the specific use of the fermentation broth.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0044] Prepare the broth:
[0045] Mortierella chinensis was inoculated in the fermentation medium, the medium components were glucose 500g, peptone 150g, yeast powder 300g, anhydrous magnesium sulfate 10g, potassium dihydrogen phosphate 20g, water 25L, cultured at 15℃100rpm for 25d, obtained fermentation broth. After the fermentation broth was centrifuged, the supernatant was taken, rotatively evaporated at 40°C, and concentrated to 1 / 10 of the original volume to obtain a 10-fold refined solution.
[0046] Insect experiment:
[0047] Select 60 bat moth larvae infected with Mortierella sinensis, and spray 30 of them with Mortierella sinensis 15-fold fermented liquid to the surface of the larvae. The amount used is 50 mL per 100 worms. The other 30 strips were treated with sterile water as controls. It was treated every 15 days. After 4 months of treatment, 30 larvae in the treatment group were still healthy and alive. Microscopic examination of samples showed that there were ...
Embodiment 2
[0050] Prepare the broth:
[0051] Mortierella chinensis was inoculated in the fermentation medium, the medium components were glucose 800g, peptone 300g, yeast powder 150g, anhydrous magnesium sulfate 15g, potassium dihydrogen phosphate 30g, water 25L, cultured at 20°C 100rpm for 14d, obtained fermentation broth. After the fermentation broth was centrifuged, the supernatant was taken, rotatively evaporated at 50°C, and concentrated to 1 / 15 of the original volume to obtain a 15-fold refined solution, weighed and then added Tween 80 20g, that is, the mass fraction of Tween 80 was 1.2%.
[0052] Insect experiment:
[0053] Select 60 bat moth larvae infected with Mortierella sinensis, and spray 30 of them with Mortierella sinensis 30-fold fermented and refined solution to the surface of the larvae, with an amount of 100 mL per 100 worms. The other 30 strips were treated with sterile water as controls. It was treated every 15 days. After 4 months of treatment, 30 larvae in the ...
Embodiment 3
[0056] Prepare the broth:
[0057] Mortierella chinensis was inoculated in the fermentation medium, the medium components were glucose 750g, peptone 250g, yeast powder 250g, anhydrous magnesium sulfate 12.5g, potassium dihydrogen phosphate 25g, water 25L, cultured at 18°C 100rpm for 20d, Obtain a fermented broth. After the fermentation broth was centrifuged, the supernatant was taken, evaporated at 45°C, and concentrated to 1 / 10 of the original volume to obtain a 10-fold refined solution, which was weighed and then 20 g of PEG was added to it, that is, the mass fraction of PEG was 0.8%.
[0058] Insect experiment:
[0059] Select 60 bat moth larvae infected with Mortierella sinensis, and spray 30 of them with Mortierella sinensis 20-fold fermented and refined solution to the surface of the larvae, with an amount of 75 mL per 100 worms. The other 30 strips were treated with sterile water as controls. It was treated every 15 days. After 4 months of treatment, 30 larvae in t...
PUM
| Property | Measurement | Unit |
|---|---|---|
| quality score | aaaaa | aaaaa |
| quality score | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More