Unlock instant, AI-driven research and patent intelligence for your innovation.

Three-dimensional bone tissue engineering scaffold material with different stiffness and preparation method of material

A technology of bone tissue engineering and scaffold materials, applied in the field of decellularized bone-collagen/hydroxyapatite three-dimensional scaffold materials and its preparation, can solve problems such as invariance, achieve the promotion of formation, and facilitate adhesion and growth , to ensure the effect of natural microstructure and ingredients

Active Publication Date: 2014-07-02
CHONGQING UNIV
View PDF6 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no three-dimensional bone tissue engineering scaffold material that can form a matrix with different stiffness while maintaining the microstructure and porosity basically unchanged.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Three-dimensional bone tissue engineering scaffold material with different stiffness and preparation method of material
  • Three-dimensional bone tissue engineering scaffold material with different stiffness and preparation method of material
  • Three-dimensional bone tissue engineering scaffold material with different stiffness and preparation method of material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0021] The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention.

[0022] Soak the cancellous bone taken from the fresh porcine femoral head with 0.9% saline at 4°C and send it to a clean condition, remove the residual muscle and tissue on the sample, and make it with a diameter of 2-20mm and a height of 2-20mm according to the clinical bone defect Cylinders of 2-20mm x 2-20mm x 2-20mm cubes and other shapes, rinse, and then soak in distilled water at 4°C overnight. The samples were treated with 1% Triton X-100 for 48h. The samples were degreased with methanol for 24 h. After incubating the sample with DNase / RNase at 37°C for 2 hours, it was washed with PBS and kept shaking. The scaffolds were immersed in absolute ethanol for 4 h to remove cell residues on the scaffolds. Wash the scaffold with a large amount of deionized water for 2 hours, dry the scaffold to obtain decellularized bone, and store i...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Hole diameteraaaaaaaaaa
Diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses a three-dimensional bone tissue engineering scaffold material with different stiffness and a preparation method of the material. The method comprises the following steps: firstly, mixing collagen with hydroxyapatite according to different ratios, so as to form different concentration gradients of collagen / hydroxyapatite mixed emulsions; immersing the fabricated acellular bone into different concentrations of mixed solutions; reperfusing and lightly vibrating, so as to form an even collagen / hydroxyapatite coating; finally freezing and drying and carrying out crosslinking treatment to obtain the three-dimensional bone tissue engineering scaffold material with different stiffness. Different stiffness of substrates can be formed on the scaffold surface under the condition that the three-dimensional scaffold microstructure is not changed, the scaffold material has good biocompatibility, proliferation and differentiation of different stem cells are facilitated, and the material is low in cost and easy to fabricate.

Description

technical field [0001] The invention relates to a bone tissue engineering scaffold material and a preparation method thereof, in particular to a decellularized bone-collagen / hydroxyapatite three-dimensional scaffold material with different stiffnesses and a preparation method thereof. Background technique [0002] In order to obtain well-organized and functional tissues, tissue engineering scaffold materials should mimic the structural and chemical characteristics of natural extracellular matrix as much as possible. A successful scaffold should mimic the natural mechanical properties in vivo and withstand the stress environment in vivo. The mechanical characteristics of scaffold materials play an important role in influencing cell behavior and controlling the transmission of external mechanical signals into cells. Cells can sense and respond to the substrate they touch, and different matrix stiffness can induce mesenchymal stem cells to differentiate into different cell typ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61L27/36A61L27/24A61L27/12
Inventor 吕永钢陈国宝邹杨
Owner CHONGQING UNIV
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com