The preparation method of parisonoside reference substance
A technique of balisonoside and reference substance, which is applied in the field of preparation of balisenside reference substance and can solve problems such as difficulty in research and the like
Inactive Publication Date: 2016-03-09
GUIZHOU UNIV
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Problems solved by technology
Although it is biased to use several chemical components as indicators to control the quality of Gastrodia elata, at present Gastrodia elata can only be purchased as gastrodin, p-hydroxybenzyl alcohol, vanillin and other synthetic products with relatively simple structure and low price. The famous and precious traditional Chinese medicine Gastrodia elata has obvious limitations
However, there are no reference substances for the more characteristic barison glycosides and polyaromatic compounds in Gastrodia elata, so it is difficult to carry out further research
Method used
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Embodiment Construction
[0052] Embodiments of the present invention: the preparation method of parisonoside reference substance, comprises the following steps:
[0053] 1) Add gastrodia elata powder to ethanol with a mass percentage of 70%, the solid-liquid ratio of gastrodia elata powder and ethanol is 1:15, the temperature of reflux extraction is 75°C-80°C, the reflux extraction is 3 times, and the time of 3 times of extraction is as follows: After 3 hours, 3 hours and 2 hours, the extracts were combined for 3 times, and the extracts were filtered; Add petroleum ether to extract and degrease, the volume ratio of the concentrated solution and petroleum ether is 1:3, and extract repeatedly 5 to 8 times until the extract is colorless and transparent, and the separated water layer is separated by a macroporous adsorption resin chromatography column, and Ethanol-water gradient elution, the elution gradient is pure water, 10% ethanol, 20% ethanol, 30% ethanol, 40% ethanol, 50% ethanol, 60% ethanol, 60% e...
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The invention discloses a method for preparing a barisenoside reference substance, which comprises extracting and separating crude barisenoside from Gastrodia elata, and then purifying by preparative liquid chromatography to prepare the barisenoside reference substance. The purity of the parisonoside reference substance prepared by the invention meets the national standard, and the route is relatively simple, reliable and easy to obtain. When its stability was investigated, the reference substance was easy to absorb moisture and decompose, and its content did not change much in dry storage at room temperature, which could be used as the conditions for the production, storage and transportation of the reference substance. The present invention provides a new technology for the preparation of barisenoside reference substance, and provides technical support for in-depth research on the quality control of Gastrodia elata.
Description
technical field [0001] The invention relates to a separation and purification technology, in particular to a preparation method of a parisonoside reference substance. Background technique [0002] Gastrodia elata (Gastrodiaelata B1.) It is a perennial herbaceous plant of Orchidaceae, distributed in most parts of the country, mainly produced in Yunnan, Guizhou, Sichuan, Shaanxi and Henan, and widely distributed in Japan and South Korea. Its dried tubers are also called Gastrodia elata, which is a commonly used and relatively expensive traditional Chinese medicine. Gastrodia elata is flat in nature, sweet in taste, and has the function of calming the liver, relieving wind and relieving convulsions. Indications headache dizziness, numbness, convulsions in children, epileptic convulsions, tetanus. Research in recent years has found that Gastrodia elata also has the functions of increasing intelligence, strengthening the brain, and delaying aging, and has a certain effect on s...
Claims
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IPC IPC(8): C07H15/203C07H1/08
Inventor 周英陈琳郭丰敏聂昌平彭礼军
Owner GUIZHOU UNIV