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Biological fermentation active carrier type composite premix feed as well as preparation method and application thereof

A technology of pre-mixed feed and biological fermentation, which is used in animal feed, animal feed, application, etc.

Inactive Publication Date: 2014-07-09
李德元 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to a series of technical problems such as the screening of microbial strains and fermentation process, there are still many problems to be solved in the production and use of microecological preparations and microbial fermented feed.

Method used

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  • Biological fermentation active carrier type composite premix feed as well as preparation method and application thereof
  • Biological fermentation active carrier type composite premix feed as well as preparation method and application thereof
  • Biological fermentation active carrier type composite premix feed as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1, liquid fermentation prepares composite probiotic bacterial liquid

[0038] ① Preparation of Lactobacillus acidophilus LA0912 strain: Inoculate the preserved Lactobacillus acidophilus LA0912 strain into an anaerobic tube containing 20mL of MRS medium, cultivate it at a constant temperature at 37°C for 20 hours to obtain the Lactobacillus acidophilus seed liquid, and then The prepared seed liquid is subjected to secondary culture according to the inoculation amount of 1-10% by volume, and the Lactobacillus acidophilus bacterial liquid for solid fermentation is obtained after standing at 37°C for 20 hours at a constant temperature for each stage;

[0039] The formula of the MRS medium is as follows: peptone, 10.0g / L; beef extract, 8.0g / L; yeast extract, 4.0g / L; glucose, 20.0g / L; Tween 80, 1mL / L; Potassium, 2.0g / L; Sodium acetate trihydrate, 5.0g / L; Triammonium citrate, 2.0g / L; Magnesium sulfate heptahydrate, 0.2g / L; Manganese sulfate, 0.05g / L; pH6.2±0.2 ;

...

Embodiment 2

[0047] Embodiment 2, solid fermentation prepares biological fermentation active carrier

[0048] ① Preparation of solid fermentation medium: 22% soybean meal, 25% corn gluten meal, 23% rice bran meal, 25% bran, and 5% brown sugar. The white matter content is weight percent.

[0049]2. The condition of solid fermentation culture: the inoculum size of the composite bacteria bacterium liquid that embodiment 1 makes in the solid fermentation medium is 3-6% (mL / g), the addition amount of protease is 100-120U / g, water The ratio to the solid fermentation medium is 1:1-0.4:1 (mL / g), the compound bacteria liquid, protease and solid fermentation medium are mixed and then fermented. The fermentation temperature is 35-40°C and the fermentation time is 72-120h . The optimal fermentation scheme obtained by multi-factor orthogonal test is: the inoculum amount of compound bacteria liquid in solid medium is 4% (mL / g), the amount of protease added is 110U / g, the ratio of water to solid medium...

Embodiment 3

[0051] Embodiment 3, the preparation of suckling piglet biological fermentation active carrier type compound premixed feed

[0052] The probiotic fermentation bioactive carrier obtained in Example 2 is compounded and uniformly mixed with various vitamins, amino acids, organic trace elements, minerals, choline chloride, and salt to obtain a suckling pig biofermentation active carrier type composite pre mixed feed. The biological fermentation active carrier type compound premixed feed for suckling piglets of the present invention contains per kilogram: copper, 360mg; iron, 1200mg; zinc, 800mg; manganese, 120mg; iodine, 1.4mg; selenium, 1mg; vitamin A, 60000IU; Vitamin E, 160IU; Vitamin K3, 16mg; Vitamin B1, 20mg; Vitamin B2, 20mg; Vitamin B6, 12mg; Vitamin B12, 0.12mg; Niacin, 200mg; Folic Acid, 6mg; Pantothenic Acid, 100mg; Choline Chloride , 2000mg; lysine, 2.8g; calcium, 65g; total phosphorus, 15g; salt, 30g.

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Abstract

The invention provides biological fermentation active carrier type composite premix feed as well as a preparation method and an application thereof. The preparation method comprises the following steps: preparing probiotics biological fermentation active carriers, to be specific, respectively carrying out liquid fermentation cultivation by using lactobacillus acidophilus LA0912, enterococcus faecalis EF1109, bacillus subtilis BS0903 and saccharomyces cerevisiae SC1203, mixing obtained liquid according to a volume ratio being 1: 1: 1: 1, then carrying out solid fermentation cultivation, drying an obtained solid fermentation culture at lower temperature and grinding so as to obtain the biological fermentation active carriers; compounding the biological fermentation active carriers with vitamins, amino acids, minerals, organic trace elements, choline chloride and table salt so as to obtain the biological fermentation active carrier type composite premix feed suitable for pigs at different stages. The biological fermentation active carrier type composite premix feed gives play to the health function of probiotics, makes the best of multiple biological activity effects of probiotics fermentation metabolites and can meet the physiologic nutritional requirements of pigs at different stages.

Description

technical field [0001] The invention relates to the technical field of microbial fermented animal feed, in particular to a biological fermentation active carrier type compound premixed feed and its preparation method and application. Background technique [0002] Over the years, due to the complexity of livestock and poultry diseases and the need for economical production of modern animal husbandry to prevent diseases and promote growth, it is common to add antibiotics to livestock and poultry feed. However, the long-term use of antibiotics in feed will have serious negative effects, which have directly or indirectly threatened human health, mainly manifested in the deterioration of the internal and external environment of breeding, the increase of resistance of pathogenic bacteria, the failure of antibacterial drugs, and the weakened immunity, etc. In recent years, the appearance of products such as enzyme preparations, plant extracts, acidifiers, and genetically engineere...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/00A23K1/18A23K10/16A23K50/30
Inventor 李德元高嘉泰
Owner 李德元
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