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Application of Lysinibacillus fs1 in Dyestuff Degradation

A technology of Bacillus lysinus and FS1, which is applied in chemical instruments and methods, textile industry wastewater treatment, water/sludge/sewage treatment, etc., to achieve the effects of low preparation cost, strong environmental adaptability, and good decolorization effect

Active Publication Date: 2015-09-30
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no research literature and patent reports on the use of Bacillus lysinus for dye decolorization in China.

Method used

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  • Application of Lysinibacillus fs1 in Dyestuff Degradation
  • Application of Lysinibacillus fs1 in Dyestuff Degradation
  • Application of Lysinibacillus fs1 in Dyestuff Degradation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1 Lysinibacillus sp . FS1 Isolation of strains obtained

[0040] 1. Strain activation and enrichment

[0041] Take 50mL of activated sludge sample (taken from the aeration tank of wastewater treatment in Guangzhou Industrial Park, Guangdong Province), put it into a conical flask filled with 50mL of sterile water (with several glass beads in it), shake it on a shaker for 30min to fully agitate Loose mud. Take 5 mL of the dispersed suspension mud sample, inoculate it into 100 mL of enrichment medium, add 30 mg / L of acid red B dye, and incubate with shaking at 37 °C and 200 rpm for 16 to 24 h.

[0042] The composition of the enrichment medium is: beef extract 1g, peptone 2g, glucose 3g, K 2 HPO 4 2g, NaH 2 PO 4 0.5g, MgSO 4 7H2O 0.2g, MnSO 4 7H2O 0.02g, FeCl 3 ·7H 2 O 0.01g, CaCl 2 0.02g, 1L sterile water, pH 7.2; sterilize the culture medium at 121°C for 15-30min.

[0043] 2. Strain screening and purification

[0044] Dilute the enriched culture ...

Embodiment 2

[0046] Example 2 Lysinibacillus sp. The decolorization effect test of FS1 on different concentrations of methylene blue

[0047] Inoculate the bacterial strain FS1 of the present invention that efficiently degrades dyes into a liquid medium, and culture it on a shaking table at 30-37° C. and 150 rpm for 10-24 hours to obtain the culture medium of the strain; the composition of the liquid medium is: Glucose 2g, ammonium chloride 1g, NaH 2 PO 4 0.5g, K 2 HPO 4 2g, FeSO 4 ·7H 2 O 0.01g, MgSO 4 ·7H 2 O 0.2g, CaCl 2 0.02g, 1L of water, pH7.2.

[0048] Take the logarithmic growth phase (OD 600 =1) of Lysinibacillus sp. FS1 culture medium was inserted into 100mL decolorized basal medium at 1% (V / V), and different concentration gradients (80mg / L, 500mg / L, 2000mg / L, 3000mg / L, 4000mg / L, 5000mg / L L) Methylene blue dye. Three parallel cultures were cultured at room temperature, and the absorbance value of the culture solution was measured every 5-24 h.

[0049] The de...

Embodiment 3

[0053] Example 3 Lysinibacillus sp. Test of decolorization effect of FS1 on different concentrations of neutral red

[0054] With reference to embodiment 2, described bacterial strain is cultivated, gets logarithmic growth phase (OD 600 =1) strain FS1 culture solution, inoculated into 100mL decolorized basal medium at 1% (V / V), and added different concentration gradients (80mg / L, 300mg / L, 500mg / L, 1000mg / L, 2000mg / L) neutral red dye. Others are the same as embodiment 2.

[0055] The test results are attached Figure 4 As shown, when the concentration of neutral red is 300-1000 mg / L, the decolorization rate basically reaches the maximum decolorization rate of about 90% in 10 hours, and the decolorization time of the strain increases by about 48 hours when the concentration increases. With the increase of the concentration of neutral red, the decolorization ability of the strain decreased gradually, from 99% to 30%. strain Lysinibacillus sp. FS1 The highest decoloriza...

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Abstract

The invention discloses a use of Lysinibacillus sp. FS1 in dye decoloring. The Lysinibacillus sp. FS1 is preserved in the China center for type culture collection (CCTCC) and has an accession number of CCTCC NO: M2013561. The Lysinibacillus sp. FS1 has a fast growth rate, strong environmental suitability, a wide-spectrum dye degradation capability, good decoloring effects on a plurality of dyes, the maximal decolourization ratio after culture for 10h, a decolourization ratio of more than 90% and the maximal decoloring concentration of 5000mg / L. The Lysinibacillus sp. FS1 can be used for decoloring treatment on printing and dyeing waste water and related dye waste water, can effectively prevent environmental pollution caused by difficultly naturally-degraded dye molecules entering into water, does not produce secondary pollution and has good ecological efficiency and application prospect. The Lysinibacillus sp. FS1 is creatively used in dye degradation, fills domestic technological blank and provides a technical basis for dye sewage treatment and dye decoloring treatment.

Description

technical field [0001] The invention relates to the field of biotechnology, more specifically, relates to the application of lysine bacillus in dye degradation. Background technique [0002] Synthetic dyes are widely used in printing and dyeing, leather, food, cosmetics, paper products and other industries, resulting in a large amount of dye wastewater. Because dye wastewater has the characteristics of high chroma, complex components, strong chemical stability, and poor biodegradability, a large amount of dyes enter the environment with the discharge of industrial wastewater, and most azo dyes have potential triple effects (teratogenicity) , carcinogenic, mutagenic), and have serious harm to the environment and human health. At present, the annual consumption of dyes in the Pearl River Delta region of my country exceeds 2×10 4 tons, of which 10-20% of the dyes are directly discharged into the environment, and azo dyes account for about 50% of the total discharge. [0003]...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F3/34C02F103/30
Inventor 杨扬苏萌陶然张敏乔永民
Owner JINAN UNIVERSITY
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