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A kind of rapid purification method of longan shell flavonoids

A technology of longan shell flavonoids and a purification method, which is applied in the field of extraction and purification of effective components of traditional Chinese medicine, can solve the problems of large solvent consumption and residual amount, limited production volume, low efficiency and the like, and achieves low solvent consumption and residual amount and low cost. low, high efficiency

Inactive Publication Date: 2016-05-11
ZHONGSHAN POLYTECHNIC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above flavonoid purification methods all have problems such as low efficiency, high cost, large solvent consumption and residue, and limited production capacity.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] (1) Extraction of flavonoids from longan shell: 10.1g of longan shell powder was extracted with 80% ethanol as the extraction solvent, and the mass-solid-liquid ratio was 1:25. (45°C constant temperature), extract twice and combine the filtrates, and then use a circulating water vacuum pump to filter at a vacuum degree of 0.1MPa for 20s, then recover ethanol with a rotary evaporator to obtain a higher concentration extract.

[0031] (2) Purification of total flavonoids of longan shell: adjust the pH value of the extract to isoelectric point 4.35 with NaOH solution and HCl solution, the flavonoids of longan shell precipitate and crystallize, and use a circulating water vacuum pump to filter under a vacuum degree of 0.1MPa for 25s to obtain flavonoids of longan shell To crystallize, wash the crystals with distilled water to remove impurities on the crystal surface, then transfer them into a small beaker and dissolve the longan shell flavonoid crystals with an 80% ethanol s...

Embodiment 2

[0033] (1) Extraction of flavonoids from longan shell: take 10.0g longan shell powder with 80% ethanol as extraction solvent, and the liquid-solid ratio is 1:25. °C and constant temperature) and extract twice and combine the filtrates. After suction filtration for 30s at a vacuum degree of 0.1 MPa with a circulating water vacuum pump, the ethanol is recovered by a rotary evaporator to obtain a higher-concentration extract.

[0034] (2) Purification of total flavonoids from longan shells: use NaOH solution and HCl solution to adjust the pH value of the extract to an isoelectric point of 4.31. Longan shell flavonoids precipitate and crystallize, and use a circulating water vacuum pump to filter under a vacuum of 0.1 MPa for 30 seconds to obtain longan shell flavonoids For crystallization, wash the crystals with distilled water to remove impurities on the crystal surface, then transfer them into a small beaker and dissolve the flavonoid crystals of longan shell with an ethanol sol...

Embodiment 3

[0036] (1) Extraction of flavonoids from longan shell: take 10.0g longan shell powder with 80% ethanol as extraction solvent, and the liquid-solid ratio is 1:25. °C constant temperature) and extract twice and combine the filtrates, use a circulating water vacuum pump to filter at a vacuum degree of 0.1MPa for 20s, and recover ethanol by a rotary evaporator to obtain a higher concentration extract.

[0037] (2) Purification of total flavonoids from longan shells: Use NaOH solution and HCl solution to adjust the pH value of the extract to an isoelectric point of 4.36. Longan shell flavonoids precipitate and crystallize, and use a circulating water vacuum pump to filter under a vacuum of 0.1 MPa for 20 seconds to obtain longan shell flavonoids To crystallize, wash the crystals with distilled water to remove impurities on the crystal surface, then transfer them into a small beaker and dissolve the longan shell flavonoid crystals in an 80% ethanol solution with a mass solid-to-liqui...

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PUM

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Abstract

The invention relates to a method for quickly purifying longan shell flavonoid, and belongs to the technical field of extraction and purification of effective components of the traditional Chinese medicine. The method concretely comprises the following steps: crystallizing and separating out longan shell flavonoid at an isoelectric point by adjusting the PH value of a longan shell flavonoid extracting solution; filtering, washing, dissolving and recrystallizing, so as to obtain high-purity longan shell flavonoid. The used reagent is a cheap non-toxic sodium hydroxide solution and a hydrochloric acid solution, and the provided method for quickly purifying the longan shell flavonoid is a method which is simple to operate, high in efficiency and low in cost.

Description

[technical field] [0001] The invention relates to a purification method of natural plant active ingredients, in particular to a rapid purification method of longan shell flavonoids, and belongs to the technical field of extraction and purification of active ingredients of traditional Chinese medicines. [Background technique] [0002] Longan Dimocarpus Longan Lour. Latin name Dimocarpus longan Lour, common name longan, is a famous subtropical fruit in my country. Longan is native to the subtropical regions in southern my country and northern Vietnam. It has been cultivated in my country for more than 2,000 years and is one of the four best fruits in Lingnan. The meat, core, flowers and roots of longan can be used as traditional Chinese medicine preparations. In the process of longan meat processing every year, a large number of longan shells are produced. These longan shells are often discarded or used as farmyard manure. Using high-efficiency and low-cost methods to extract...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/77A61K47/02A61K47/04A61K47/10
Inventor 韩淑琴吴丽李志锐
Owner ZHONGSHAN POLYTECHNIC