Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for separating immune globulin antibody

A technology for immunoglobulin and separation methods, which is applied in the field of separating immunoglobulin antibodies from milk by using anionic crystal gel adsorption chromatography technology, which can solve the problems of long production cycle, complicated industrial process, difficult adsorption, etc., and achieve large processing capacity , The process is simple, the effect of large adsorption capacity

Inactive Publication Date: 2014-07-23
SHIHEZI UNIVERSITY
View PDF0 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The industrial process of extracting IgG from whey mainly includes multiple combined steps such as centrifugation, salting out, ultrafiltration, concentration and anion exchange chromatography. The industrial process is complex and the production cycle is long.
When conventional anion exchange resin chromatography is used to extract IgG from whey, since the pore size is in the nanoscale range, it is difficult to adsorb macromolecular IgG in the chromatography of multi-component proteins

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] [Example 1] Get 5 mL of bovine whey, dilute it 6 times with 20 mM phosphate buffered saline (PBS, pH=5.8) and deionized water, carry out crystal gel continuous bed chromatography with a flow rate of 1 cm / min, then buffer with PBS Wash with NaCl, and carry out gradient elution with the eluent (buffer configuration) containing NaCl respectively 40mM, 100mM, 200mM, 300mM, and 1M, collect the elution peak corresponding to the 40mM eluent, and obtain the immunoglobulin antibody IgG; The purity detected by SDS-PAGE electrophoresis is about greater than 95%, and the total protein adsorption capacity reaches 0.259 mg / mL bed layer.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
purityaaaaaaaaaa
Login to View More

Abstract

The invention discloses a method for separating an immune globulin antibody. The method comprises the following steps: 1, diluting a raw material containing immune globulin by using a buffer solution of which the pH value is 4.0-8.0 and deionized water to obtain a loading fluid; 2, sampling the preprocessed loading fluid to a crystal gel column at the flow rate of 0.1-20cm / min to carry out adsorption; 3, with the deionized water or the buffer solution of which the pH value is 4.0-8.0 as the washing fluid, flushing un-adsorbed impurities inside the crystal gel column; and 4, with the buffer solution of which the pH value is 4.0-8.0 and contains 0.01-4M of alkali metal salt as an eluent, carrying out gradient elution, and collecting an eluting peak containing the immune globulin to obtain the immune globulin. According to the method for separating immune globulin, which is disclosed by the invention, the used anion exchange type crystal gel medium has ultra-large pores, realizes convective diffusion inside the pores and high-purity IgG (immunoglobulin G) separation, and is high in mass transfer efficiency; the method disclosed by the invention is simple in craft process, good in adaptability and low in extraction cost.

Description

technical field [0001] The invention relates to a method for isolating immunoglobulin antibody (IgG), in particular to a method for isolating immunoglobulin antibody from milk by using anion crystal gel adsorption chromatography technology. Background technique [0002] IgG is ubiquitously present in the blood, interstitial fluid and exocrine fluid of animals, among which animal milk is an important source of IgG, which plays an important role in immune and physiological regulation. The advantage of milk as a raw material for production is that the raw material is abundant and cheap, and the IgG content in bovine whey is relatively high (about 9.6%-10.8%). The industrial process of extracting IgG from whey mainly includes multiple combined steps such as centrifugation, salting out, ultrafiltration, concentration and anion exchange chromatography. The industrial process is complex and the production cycle is long. When conventional anion exchange resin chromatography is used...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/04C07K1/22
Inventor 代斌贠军贤陈良张海燕董莎莎
Owner SHIHEZI UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com