Method for separating immune globulin antibody

Abstract

The invention discloses a method for separating an immune globulin antibody. The method comprises the following steps: 1, diluting a raw material containing immune globulin by using a buffer solution of which the pH value is 4.0-8.0 and deionized water to obtain a loading fluid; 2, sampling the preprocessed loading fluid to a crystal gel column at the flow rate of 0.1-20cm/min to carry out adsorption; 3, with the deionized water or the buffer solution of which the pH value is 4.0-8.0 as the washing fluid, flushing un-adsorbed impurities inside the crystal gel column; and 4, with the buffer solution of which the pH value is 4.0-8.0 and contains 0.01-4M of alkali metal salt as an eluent, carrying out gradient elution, and collecting an eluting peak containing the immune globulin to obtain the immune globulin. According to the method for separating immune globulin, which is disclosed by the invention, the used anion exchange type crystal gel medium has ultra-large pores, realizes convective diffusion inside the pores and high-purity IgG (immunoglobulin G) separation, and is high in mass transfer efficiency; the method disclosed by the invention is simple in craft process, good in adaptability and low in extraction cost.

Description

technical field [0001] The invention relates to a method for isolating immunoglobulin antibody (IgG), in particular to a method for isolating immunoglobulin antibody from milk by using anion crystal gel adsorption chromatography technology. Background technique [0002] IgG is ubiquitously present in the blood, interstitial fluid and exocrine fluid of animals, among which animal milk is an important source of IgG, which plays an important role in immune and physiological regulation. The advantage of milk as a raw material for production is that the raw material is abundant and cheap, and the IgG content in bovine whey is relatively high (about 9.6%-10.8%). The industrial process of extracting IgG from whey mainly includes multiple combined steps such as centrifugation, salting out, ultrafiltration, concentration and anion exchange chromatography. The industrial process is complex and the production cycle is long. When conventional anion exchange resin chromatography is used...

AI Technical Summary

Problems solved by technology

The industrial process of extracting IgG from whey mainly includes multiple combined steps such as centrifugation, salting out, ultrafiltration, concentration and anion exchange chromatography. The industrial process is complex and the production cycle is long.

When conventional anion exchange resin chromatography is used to extract IgG from whey, since the pore size is in the nanoscale range, it is difficult to adsorb macromolecular IgG in the chromatography of multi-component proteins