A strain of Serratia marcescens m7a and its application in removing heavy metal ions
A Serratia marcescens, heavy metal ion technology, applied in bacteria, microorganism-based methods, restoration of polluted soil, etc., can solve the problem of bacterial strain screening, less research on bacterial strain characteristic growth conditions, and the ability to remove heavy metals Not strong, few heavy metal populations, etc.
Active Publication Date: 2016-01-20
润桐(苏州)技术服务有限公司
View PDF2 Cites 0 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
In the past ten years, the research on heavy metal-resistant microorganisms in my country has mainly focused on the properties of microorganisms and the influence of heavy metals on bacterial cells, while there are few studies on strain screening, strain characteristics, and growth conditions.
At present, there are few isolated heavy metal-resistant populations, the growth and reproduction of bacteria are greatly affected by the habitat environment, and the ability to remove heavy metals is not strong, and it cannot meet the complex pollution status of the external actual environment. ability to remove
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment Construction
[0111] Remove heavy metal ions Mn 2+ strain screening
[0112] 1 Experimental materials
[0113] 1.1 Experimental strains
[0114] Sample source
[0115] Soil or water samples from different locations were collected from Weifang, Rizhao, Nanjing and other areas. The soil samples were collected from 1-4cm topsoil in multiple places in the sampling area and mixed separately. There are 27 samples in total.
[0116] 1.2 Experimental Instruments
[0117] Vertical pressure steam sterilizer (HirayamaHVE-50, Japan); air bath shaker (IKAKS4000ic, Germany); analytical balance (MettlerToledoPL602-S, Switzerland); constant temperature incubator (MMMIncucell111, Germany); pipette gun (Eppendorf, Germany); Centrifuge (Eppendorf 5417R, Germany); Inductively Coupled Plasma Mass Spectrometry (ICP-MS, Agilent 7700X, USA), etc.
[0118] 1.3 Experimental reagents
[0119] Manganese chloride [MnCl 2 ×4H 2 O] Chemical reagents were of analytical grade. Calculate the mass fraction of metal...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
The present invention discloses a strain of Serratia marcescens M7a, and an application of the Serratia marcescens M7a in heavy metal ion removing, wherein the Serratia marcescens M7a provides a heavy metal ion Mn<2+> removing effect. The method for screening Achromobacter xylosoxidans providing a heavy metal ion Mn<2+> removing effect from soil and water comprises: a, preparing a metal selective culture medium; b, carrying out enrichment acclimatization on a heavy metal resistance strain; c, screening and re-screening the heavy metal resistance strain; and d, detecting the heavy metal removing capability of the strain. According to the present invention, with the detection test on the heavy metal removing capability of the strain obtained by carrying out enrichment acclimatization on the heavy metal resistance strain with the metal ion-containing culture medium and purification, the reasonable and effective screening method for the heavy metal removing strain is established so as to obtain the strain suitable for requirements; and with application of the Serratia marcescens M7a in the heavy metal pollution treatment, advantages of no secondary pollution, high treatment efficiency, wide application range, low cost and the like are provided.
Description
technical field [0001] The invention relates to a strain of microorganism and its application in removing heavy metal ions, in particular to a strain of Serratia marcescens and its application in removing heavy metal pollution, belonging to the technical field of detection and application of microorganisms. Background technique [0002] Heavy metals are widely distributed in the atmosphere, biosphere, lithosphere, and hydrosphere. After being discharged into the environment, they are permanent and have obvious cumulative effects. They can pollute water bodies and soils and eventually accumulate in organisms through food chain cycles, resulting in serious Harm the ecological environment and human health. [0003] The technology of microbial treatment of heavy metal pollution is an emerging field that is constantly being explored and developed in the world. The use of biosorbents prepared by microbial systems to treat heavy metal pollution is currently the most promising metho...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34B09C1/10C12R1/43C02F101/20
Inventor 赵晗田国宁马荣桧王亮亮
Owner 润桐(苏州)技术服务有限公司