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A kind of fresh Bdellovibrio microbial preparation and the method for rapidly evaluating its shelf life

A technology for microbial preparation and leech vibrio, which is applied in the field of microbial preparations, can solve the problems of insufficient vigor of leech vibrio, limited application of leech vibrio, etc., and achieves the effects of convenient storage and transportation, low cost and simple operation.

Inactive Publication Date: 2016-10-05
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The primary purpose of the present invention is to overcome the shortcomings and deficiencies that the existing Bdellovibrio viability is not kept long enough to cause the extensive application of Bdellovibrio to be limited, and to provide a fresh Bdellovibrio microbial preparation

Method used

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  • A kind of fresh Bdellovibrio microbial preparation and the method for rapidly evaluating its shelf life
  • A kind of fresh Bdellovibrio microbial preparation and the method for rapidly evaluating its shelf life
  • A kind of fresh Bdellovibrio microbial preparation and the method for rapidly evaluating its shelf life

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] 1. Preparation of Bdellovibrio microbial preparations

[0047] (1) Preparation of host bacteria suspension

[0048] Aeromonas hydrophila was purchased from the Culture Collection Center of Guangdong Institute of Microbiology, number GIM1.172. Pick a ring of bacteria from the preserved nutrient agar medium plate marked with Aeromonas hydrophila, and inoculate it in sterile nutrient broth medium (nutrient broth 18g, distilled water 1000mL, pH7.2), Shake at 200rpm and 28°C for 18h, then centrifuge the culture solution at 4°C and 6000rpm for 20min, discard the supernatant, suspend the precipitate with 2-3mL nutrient broth liquid medium; obtain Aeromonas hydrophila suspension, 4°C Store in the refrigerator for later use.

[0049] (2) Preparation of swimming bodies of Bdellovibrio BDS01

[0050] The Bdellovibrio swimming body is fermented by the fermentation method of the high-density Bdellovibrio swimming body disclosed in the national invention patent application with th...

Embodiment 2

[0090] 1. Preparation of Bdellovibrio microbial preparations

[0091](1) Preparation of host bacteria suspension

[0092] Aeromonas hydrophila was purchased from the Culture Collection Center of Guangdong Institute of Microbiology, number GIM1.172. Pick a ring of bacteria from the preserved nutrient agar medium plate marked with Aeromonas hydrophila, and inoculate it in sterile nutrient broth medium (nutrient broth 18g, distilled water 1000mL, pH7.2), Shake at 200rpm and 28°C for 18h, then centrifuge the culture solution at 4°C and 6000rpm for 20min, discard the supernatant, suspend the precipitate with 2-3mL nutrient broth liquid medium; obtain Aeromonas hydrophila suspension, 4°C Store in the refrigerator for later use.

[0093] (2) Preparation of swimming bodies of Bdellovibrio BDS01

[0094] The Bdellovibrio swimming body is fermented by the fermentation method of the high-density Bdellovibrio swimming body disclosed in the national invention patent application with the...

Embodiment 3

[0111] Constant temperature storage verification test

[0112] The better group (group 6) calculated in Example 2 was placed in an incubator at 25°C and 4°C for verification test. The bacterial solution at each temperature was done in 3 parallels, and the average value was taken. The concentration of viable bacteria was detected every 12 hours at 25°C, and every 10 days at 4°C.

[0113] 1. Confirmatory test results at 25°C

[0114] After 120h of storage, the order of magnitude of BDS01 at 25℃ is still within 2 orders of magnitude lower than that at the beginning.

[0115] 2. Confirmatory test results at 4°C

[0116] After the 11th detection, the active BDS01 bacterial liquid could still be detected in the range of 2 orders of magnitude lower than the initial concentration.

[0117] The results of the constant temperature storage verification test show that the number of viable bacteria can still be detected during the shelf life predicted in Example 2, that is, the results ...

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Abstract

The invention provides a preparation of freshwater bdellovibrio-and-like organisms and a method for rapidly evaluating shelf life of the preparation. The prepared preparation of freshwater bdellovibrio-and-like organisms helps to substantially improve the survival rate of bdellovibrio-and-like organisms, and provides new solution thinking and method about the problem of bad vitality of bdellovibrio-and-like organisms, and enables bdellovibrio-and-like organisms to be easily cultured and stored for a long time, and thus the preparation is possible widely applied to culture of aquatic livestocks. The disclosed method for rapidly evaluating shelf life of the preparation of freshwater bdellovibrio-and-like organisms is capable of rapidly evaluating vitality keeping circumstance of freshwater bdellovibrio-and-like organisms, the method is simple in operation and accurate in data, no traditional analysis detection is needed, and existed data can be utilized for predicting the activity of microbes.

Description

technical field [0001] The invention relates to a microbial preparation, in particular to a Bdellovibrio microbial preparation and a method for rapidly evaluating its shelf life. Background technique [0002] Bdellovibrio-and-like organisms (BALOs) are a type of bacteria that parasitize other bacteria and rely on lysing host bacteria to grow and reproduce themselves. It has the physiological characteristics of parasitic and lysing bacteria, especially for many pathogenic Vibrio Has a good cracking effect. It has broad application prospects in controlling bacteria and in aquaculture. The use of Bdellovibrio to control pathogenic bacteria in aquaculture has been a research hotspot in recent years. Bdellovibrio has a strong ability to lyse most pathogenic bacteria, can improve water quality, is safe and environmentally friendly, and may replace antibiotics as a control agent. new approaches to disease. Most of Bdellovibrio have the problem that it is not easy to preserve, wh...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12Q1/04C12R1/01
Inventor 蔡俊鹏田双娥
Owner SOUTH CHINA UNIV OF TECH