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Bacillus circulans and microbial agents and their applications

A technology of Bacillus circulans and microbial inoculants, applied in the field of agricultural biology, can solve problems such as poor effect of tomato gray mold

Active Publication Date: 2017-01-04
YANTAI DIYUAN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to overcome the defect that the existing microbial strains are less effective in preventing and treating gray mold of tomato, the present invention provides a kind of Bacillus circulans, and the preservation number of the Bacillus circulans is CGMCC NO.8910

Method used

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  • Bacillus circulans and microbial agents and their applications
  • Bacillus circulans and microbial agents and their applications
  • Bacillus circulans and microbial agents and their applications

Examples

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preparation example Construction

[0017] Wherein, the preparation method of the microbial agent may include: inoculating the Bacillus circulans of CGMCC No.8910 in the fermentation medium for cultivation. Preferably, in each gram of the microbial bacterial agent obtained by culturing, the number of live bacteria of Bacillus circulans with a preservation number of CGMCC NO.8910 can be (1-50)×10 7 CFU, more preferably (2-20)×10 7 CFU. Wherein, the cultivation temperature may be 30-35°C, and the cultivation time may be 24-48 hours.

[0018] The present invention also provides the application of the above-mentioned bacillus circulans and the above-mentioned microbial agent in preventing and controlling crop diseases.

[0019] Particularly preferably, the crop diseases include botrytis cinerea caused by Botrytis cinerea. For example, the botrytis includes tomato botrytis, cucumber botrytis, grape botrytis, strawberry botrytis, lettuce botrytis, eggplant botrytis, pepper botrytis, peach botrytis and At least one...

Embodiment 1

[0022] This example is used to illustrate the cultivation of Bacillus circulans and the preparation of microbial inoculum of the present invention.

[0023] Inoculate Bacillus circulans with the preservation number CGMCC NO.8910 into LB medium (containing 10g / L tryptone, 5g / L yeast extract and 10g / L NaCl), at 30°C and 180 rev / min shaking culture to bacterial density OD 600 The value is 0.6-0.8, and the seed bacterial liquid is obtained.

[0024] Add 100mL of the above-mentioned seed bacterial liquid into 100L of fermentation medium (fermentation medium contains 30g / L of sucrose, 3g / L of bean cake powder, 2g / L of ammonium sulfate, 0.2g / L of yeast powder, 0.8g / L of Diammonium hydrogen phosphate, magnesium chloride 0.08g / L and sodium chloride 0.1g / L), cultured at 30°C, samples were taken during the cultivation and observed by direct counting under a microscope until the amount of The viable count of Bacillus circulans is 10 8 CFU, the resulting culture solution is the microb...

Embodiment 2

[0026] Prepare the microbial agent according to the method of Example 1, the difference is that the fermentation medium used is to contain the starch of 5g / L, the sucrose of 1g / L, the calcium carbonate of 1g / L, the ammonium sulfate of 2g / L, the ammonium sulfate of 1g / L L of potassium dihydrogen phosphate and 0.5g / L of magnesium chloride culture medium.

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Abstract

The invention provides a bacillus circulans. The collection number of the bacillus circulans is CGMCC No. 8910. The invention also provides a microbial agent which contains bacteria and a culture medium; the bacteria comprise the bacillus circulans of which the collection number is CGMCC No. 8910. The invention also provides application of the bacillus circulans and the microbial agent in preventing and treating diseases of crops. Due to the adopted technical scheme, the bacillus circulans and the microbial agent are capable of preventing and treating the diseases of crops such as tomato gray mold.

Description

technical field [0001] The invention relates to the field of agricultural biotechnology, in particular to a bacillus circulans, a microbial agent and applications thereof. Background technique [0002] Botrytis cinerea is a serious and common disease on tomatoes, which occurs in all vegetable areas. In addition to harming tomatoes, it can also harm more than 20 kinds of crops such as eggplants, peppers, cucumbers, and gourds. Years with low temperature and continuous rainy weather are serious hazards. When the disease is severe, the stems and leaves die and a large number of rotten flowers and fruits are caused, which directly affects the yield. Tomatoes infected by Botrytis cinerea generally have a 20%-30% yield reduction, sometimes as much as 50% or so, and even cause tomato extinction in severe cases. The causative agent of tomato gray mold is Botrytis sp. The pathogen mainly uses sclerotia (cold regions) or mycelium and spores (warm regions) to survive the summer or ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/09
Inventor 赵君才阮志勇赵秉强王慧敏赵文勇吕昭辉刘慧宋昭王彦伟刘小飞
Owner YANTAI DIYUAN BIOTECH