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Ganoderma lucidum polysaccharide, extraction and purification method and its application as tobacco humectant

A technology of Ganoderma lucidum polysaccharide and humectant, which is applied in the fields of tobacco, application, and treatment of tobacco, etc., to achieve the effect of obvious moisturizing effect, broad promotion and application prospects, and easy operation

Active Publication Date: 2016-06-29
CHINA TOBACCO HENAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no relevant report on the application of Ganoderma lucidum polysaccharide in cigarettes as a tobacco humectant at home and abroad

Method used

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  • Ganoderma lucidum polysaccharide, extraction and purification method and its application as tobacco humectant
  • Ganoderma lucidum polysaccharide, extraction and purification method and its application as tobacco humectant

Examples

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Embodiment 1

[0018] A method for extracting and purifying Ganoderma lucidum polysaccharides, comprising the following steps: extract 300 g of dried Ganoderma lucidum with 1800 ml of ether Soxhlet for 2 hours to remove oil, and evaporate the residue to dryness. The residue after evaporating to dryness was extracted three times with 750ml boiling water, combined the boiling water extracts obtained three times, concentrated, centrifuged, and the supernatant was taken, and the supernatant was added with absolute ethanol 3 times the volume of the clear liquid for alcohol precipitation, and stood overnight (12h), again. The precipitate was washed three times with 300ml ethanol and 300ml acetone in turn, and then dissolved in distilled water, then papain with a weight of 1‰ of the precipitate was added and placed in a water bath at 50°C for 2 hours, and the protein was removed by the Sevage method. Add 8g of activated carbon and place it in a 70°C water bath to heat for 3 hours, remove the activa...

Embodiment 2

[0021] A method for extracting and purifying Ganoderma lucidum polysaccharides, comprising the following steps: extract 300 g of dried Ganoderma lucidum with 2000 ml of ether Soxhlet for 4 hours to remove oil, and evaporate the residue to dryness. The residue after evaporating to dryness was extracted with 750ml of boiling water for 3 times, and the boiling water extracts obtained three times were combined, concentrated, centrifuged, and the supernatant was taken, and anhydrous ethanol with 3 times the volume of the clear liquid was added for alcohol precipitation, and stood for 12 hours , and centrifuge again to collect the precipitate. The precipitate was washed three times with 300ml of ethanol and 300ml of acetone in turn, then dissolved in distilled water, and then papain with a weight of 2‰ of the precipitate was added, placed in a water bath at 50°C for 4 hours, and the protein was removed by Sevage method. Add 8g of activated carbon and place it in a 70°C water bath to...

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Abstract

The invention relates to a Ganoderma lucidum polysaccharide and a method for extracting and purifying the ganoderma lucidum, specifically: after extracting the pulverized dry Ganoderma lucidum with ether Soxhlet, the residue is evaporated to dryness and then extracted with boiling water, the boiling water extract is concentrated and centrifuged, and the supernatant alcohol is taken Stand still, collect the precipitate by centrifugation again, dissolve it with distilled water after washing, then add 1-2‰ papain and place it in a water bath at 50±5°C for 2-4 hours, use the Sevage method to remove the protein, decolorize with activated carbon, and concentrate under reduced pressure. After cooling, carry out running water dialysis. After the dialysis is completed, let it stand for alcohol precipitation again, and dry the precipitate at 60-70°C to obtain crude polysaccharide. Tests have proved that the Ganoderma lucidum polysaccharide of the present invention has a good physical and sensory moisturizing effect on cigarettes, and can reduce the irritation and miscellaneous gas of cigarettes, improve the roundness and comfort of cigarette smoke, and can be used as a tobacco with excellent performance. Moisturizers are used in cigarettes.

Description

technical field [0001] The invention belongs to the technical field of humectants, and in particular relates to a ganoderma lucidum polysaccharide, an extraction and purification method and its application as a tobacco humectant. Background technique [0002] At present, glycerin, propylene glycol, sorbitol and other polyhydroxy substances are mainly used as humectants in the cigarette industry in my country. The main purpose of using such humectants is to maintain the moisture content of shredded tobacco during processing and improve the processing resistance of shredded tobacco. The above-mentioned humectant has unsatisfactory effects on maintaining the moisture content of finished cigarettes and improving smoking comfort. [0003] Polysaccharides are composed of a large number of monosaccharides, and a large number of hydroxyl groups form hydrogen bonds with water molecules, which can make them have strong water holding capacity. At present, there are no relevant reports ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A24B15/40
Inventor 陈芝飞郝辉孙志涛芦昶彤刘强蔡莉莉宋金勇周浩马宇平聂聪
Owner CHINA TOBACCO HENAN IND
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