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Device for separating and extracting virus in water and method for extracting sapovirus in water

An extraction device and virus separation technology, which is applied in the field of water virus separation and extraction device, can solve the problems such as easy air leakage of microbial filters, and achieve the effect of reducing the content of interfering components, reducing the content of impurities, and reducing the impact of loss

Active Publication Date: 2014-08-13
INST OF MICROBIOLOGY HEILONGJIANG ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The present invention solves the technical problem of easy air leakage in existing microbial filters; and provides a water virus separation and extraction device with good sealing performance, good virus filtering effect in water, easy disassembly and assembly, and easy repeated sterilization and use

Method used

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  • Device for separating and extracting virus in water and method for extracting sapovirus in water
  • Device for separating and extracting virus in water and method for extracting sapovirus in water
  • Device for separating and extracting virus in water and method for extracting sapovirus in water

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specific Embodiment approach 3

[0029] Specific implementation mode three: combination figure 1 , 2 As described with 3, the difference between this embodiment and the first embodiment is that the positive charge filter membrane is a NanoCeram positive charge filter membrane. The other structure and connection mode are the same as the first embodiment.

specific Embodiment approach 4

[0030] Specific embodiment four: This embodiment adopts the device in specific embodiment three for filtration and elution. The specific extraction method is carried out according to the following steps:

[0031] Step 1. Load the water sample into the storage tank 2, close the top cover, and introduce nitrogen. The pressure in the storage tank 2 rises to 0.7 standard atmospheric pressure, and the control valve is opened to allow the water sample to flow through the filter to the membrane support. After all the water samples have passed through the positively charged filter membrane in the filter membrane holder, the injection is stopped, the nitrogen storage tank is closed, the tank body is depressurized, and a new storage tank is replaced;

[0032] Step 2. Add the beef extract solution with a pH value of 9.5-9.75 to the replaced storage tank, and then pass nitrogen gas. The pressure in the storage tank 2 rises to 0.7 standard atmospheric pressure, and the control valve is opened to...

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Abstract

The invention relates to a device for separating and extracting a virus in water and a method for extracting sapovirus in water and belongs to the field of extraction of viruses in the water. The invention aims at solving the technical problem that the existing microbiological filter can leak the air easily and provides a method for extracting the sapovirus in the water with high extraction efficiency, less product extraction interference and extracted product applicable to multiple detecting platforms. The device for separating and extracting the virus in the water is characterized in that a storage tank is provided with a dome, an arc-shaped throat is formed in the dome, an edge rolling outside is formed at the bottom of a top cover, the top cover is inserted inside from the arc-shaped throat and is blocked in the arc-shaped throat by virtue of a sealing ring, lugs are symmetrically arranged at the top of the top cover, a lever is respectively arranged in each lug, a water outlet pipe penetrates through the dome to be inserted into the bottom of the storage tank, the air inlet pipe is connected with a nitrogen pressure tank, the water outlet pipe is connected with a filter, and the filter is connected with a filter membrane support. The method for extracting the sapovirus in the water comprises the following steps: filtering a water sample, eluting with a beef extract solution, treating by adopting an isoelectric point sedimentation method and then centrifuging, remelting and carrying out secondary concentration, and then extracting nucleic acid, so that extraction of the sapovirus is completed. The device and method provided by the invention are used for separating and extracting the virus in the water.

Description

Technical field [0001] The invention belongs to the field of water virus extraction; in particular, it relates to a water virus separation and extraction device and a method for extracting Sapporo virus in water by using the device. Background technique [0002] The risk of viruses in water to human health has attracted increasing attention. The detection of viruses in environmental waters is a valuable task to prevent diseases, evaluate water sanitation quality and environmental sanitation. [0003] The overlap between the inner tank and the upper cover of the existing water microbial filter is not good, and as the pressure increases, the airtightness of the upper cover decreases, and air leakage is easy. [0004] Gastrointestinal diseases caused by viruses are extremely harmful to humans. Sapporo virus (Sapovirus / SaV) is a common virus that causes acute diarrhea. The virus was discovered during an intestinal disease outbreak in Sapporo, Japan in 1977. SaV belongs to the caliciviru...

Claims

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Application Information

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IPC IPC(8): C12M1/12C12N7/02C12N15/10C12Q1/70C12Q1/68C12R1/93
CPCC12M47/02C12N7/00C12N2770/16051
Inventor 原韬夏海华于冲王金英曲晓军孙建华
Owner INST OF MICROBIOLOGY HEILONGJIANG ACADEMY OF SCI
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