Self-set concentration gradient drug screening organ chip and preparation method thereof

An organ chip and concentration gradient technology, applied in the field of biomedicine, can solve the problems of single use of cell culture system, not easy to miniaturize and integrate, and expensive, etc., and achieve the effect of wide detection range, low drug consumption, and reduced demand.

Inactive Publication Date: 2014-08-13
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to provide a self-set concentration gradient drug screening organ chip and its preparation method. The organ chip performs parallel co-culture of multiple cells and interacts with drugs to evaluate the safety and effectiveness of drugs with multiple concentration gradients. It solves the traditional drug screening using animal models, which is expensive, time-consuming, and ethically controversial, and solves the shortcomings of the existing cell culture system, which is single-purpose and difficult to miniaturize and integrate.

Method used

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  • Self-set concentration gradient drug screening organ chip and preparation method thereof

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Effect test

Embodiment 1

[0026] Example 1 Self-set concentration gradient drug screening organ chip preparation

[0027] Use computer-aided design software to design and draw the microstructure and microchannel graphics of the self-set concentration gradient drug screening organ chip. First, use the silicon deep etching technology in micro-electromechanical processing to make silicon molds, or spin-coat photoresist on the film, The photoresist mold is formed after photolithography and self-development, and the microstructure and microchannel of the chip can also be processed and prepared by the numerical control CNC system. Mix the prepolymer of polydimethylsiloxane (PDMS) with the curing agent, and cast it on the mold after ultrasonic, stirring, vacuum degassing and other steps, heat reaction at an appropriate temperature for a certain period of time, cool naturally, and polydimethylsiloxane The silicone-based (PDMS) chip was carefully peeled off from the mold, and aligned, glued, and pressure-sewn w...

Embodiment 2

[0028] Example 2 Self-set concentration gradient drug screening organ chip preparation

[0029]Using computer-aided design software to design and draw the microstructure and microchannel graphics of the self-set concentration gradient drug screening organ chip, and use the numerical control CNC system to process and prepare the microstructure and microchannel of the three-layer polymethacrylic acid (PMMA) chip. Clean the chips of each layer with tap water and distilled water respectively, and wipe away fingerprints, oil stains and other stains remaining on the chip surface with ethanol. On the double-sided adhesive film, the microstructures and microchannels required for the preparation of the chip are processed with a plotter, and the chip substrate and the cover are aligned, glued, and pressure-sewn to make 8 repeated cell culture units and 2 drugs. Supply system, 1 blank control system and 1 co-culture system for drug screening organ chip with self-set concentration gradien...

Embodiment 3

[0030] Implantation and co-cultivation of embodiment 3 cells

[0031] Close the import and export of drugs and medium, open the import and export of the seed cell culture system, and connect the import and export of every four gradient units with additional pipelines, so as to connect the four units. The cell solution is injected into the seed cells in the inlet, and the laminar flow velocity is controlled so that the cell solution slowly flows through the microchannel into the cell culture chamber, thereby completing the implantation of the seed cells at one time. Close the import and export of the seed cell culture system, open the import and export of the co-culture system, and complete the implantation of cells. The blank control system followed the same steps to complete the implantation and culture of cells. The cells are cultured for a period of time, and after they grow on the wall or three-dimensional fixation, the culture medium is circulated and flowed. The growth ...

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Abstract

The invention relates to a self-set concentration gradient drug screening organ chip and a preparation method thereof. The organ chip comprises a multi-cell co-culture system, a seed cell culture system, a blank control system and a drug testing system. The organ chip simultaneously can carry out activity or toxicity test and blank control experiment on drugs of eight drug concentration gradients at a time, so that the organ chip is simple in operation, can realize the parallel implantation and co-culture of various cells, reduce the application amount of actual samples, implement low drug consumption, and simplify a cell implantation process, therefore, the organ chip has portable, economical, efficient and accurate characteristics. The organ chip can independently carry out cell growth and culture and carry out multi-concentration gradient detection on drugs, and can carry out in-vitro, real-time and online observation on the biological behaviors of cells, tissues and organs, thereby providing a brand-new technical platform for cell-drug research and high-throughput drug screening.

Description

technical field [0001] The invention relates to an organ chip and a preparation method thereof, in particular to an organ chip for self-set concentration gradient drug screening and a preparation method thereof. It belongs to the technical field of biomedicine. Background technique [0002] The organ chip is a three-dimensional cell culture system based on a multi-channel fluid chip, which is composed of multiple cell culture partitions that simulate the environment of human tissues and organs. Connections are made in each zone through a bionic circulatory system. The organ chip also contains integrated micro-sensing and imaging devices for real-time and online detection of the microenvironment and growth status of the growth of three-dimensional cell aggregates, the interaction between tissues and organs, etc. Its main goal is to simulate the environment of organisms on the chip to cultivate cells, tissues and organs, study and control the biological behavior of cells in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/34
CPCB01L3/5027B01L2200/10B01L2300/12G01N33/5008G01N33/94G01N2500/10
Inventor 顾忠泽郑付印赵远锦程瑶刘慈慧汤栋梁王洁
Owner SOUTHEAST UNIV
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