Self-set concentration gradient drug screening organ chip and preparation method thereof
An organ chip and concentration gradient technology, applied in the field of biomedicine, can solve the problems of single use of cell culture system, not easy to miniaturize and integrate, and expensive, etc., and achieve the effect of wide detection range, low drug consumption, and reduced demand.
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Embodiment 1
[0026] Example 1 Self-set concentration gradient drug screening organ chip preparation
[0027] Use computer-aided design software to design and draw the microstructure and microchannel graphics of the self-set concentration gradient drug screening organ chip. First, use the silicon deep etching technology in micro-electromechanical processing to make silicon molds, or spin-coat photoresist on the film, The photoresist mold is formed after photolithography and self-development, and the microstructure and microchannel of the chip can also be processed and prepared by the numerical control CNC system. Mix the prepolymer of polydimethylsiloxane (PDMS) with the curing agent, and cast it on the mold after ultrasonic, stirring, vacuum degassing and other steps, heat reaction at an appropriate temperature for a certain period of time, cool naturally, and polydimethylsiloxane The silicone-based (PDMS) chip was carefully peeled off from the mold, and aligned, glued, and pressure-sewn w...
Embodiment 2
[0028] Example 2 Self-set concentration gradient drug screening organ chip preparation
[0029]Using computer-aided design software to design and draw the microstructure and microchannel graphics of the self-set concentration gradient drug screening organ chip, and use the numerical control CNC system to process and prepare the microstructure and microchannel of the three-layer polymethacrylic acid (PMMA) chip. Clean the chips of each layer with tap water and distilled water respectively, and wipe away fingerprints, oil stains and other stains remaining on the chip surface with ethanol. On the double-sided adhesive film, the microstructures and microchannels required for the preparation of the chip are processed with a plotter, and the chip substrate and the cover are aligned, glued, and pressure-sewn to make 8 repeated cell culture units and 2 drugs. Supply system, 1 blank control system and 1 co-culture system for drug screening organ chip with self-set concentration gradien...
Embodiment 3
[0030] Implantation and co-cultivation of embodiment 3 cells
[0031] Close the import and export of drugs and medium, open the import and export of the seed cell culture system, and connect the import and export of every four gradient units with additional pipelines, so as to connect the four units. The cell solution is injected into the seed cells in the inlet, and the laminar flow velocity is controlled so that the cell solution slowly flows through the microchannel into the cell culture chamber, thereby completing the implantation of the seed cells at one time. Close the import and export of the seed cell culture system, open the import and export of the co-culture system, and complete the implantation of cells. The blank control system followed the same steps to complete the implantation and culture of cells. The cells are cultured for a period of time, and after they grow on the wall or three-dimensional fixation, the culture medium is circulated and flowed. The growth ...
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