A kind of preparation method of nano-gold immune chromatography capillary

A technology of immunochromatography and capillary, which is applied in the field of immunoassay, can solve the problems of complex substrate structure of paper materials, complex assembly and detection process, difficult control of fixed amount and boundary, etc., and achieves simple and easy to master assembly technology, low cost, The effect of reducing inter-assay and intra-assay variation

Active Publication Date: 2015-11-18
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the substrate structure of paper materials is still relatively complex and the paper substrate is brittle and easily broken during assembly.
In addition, cotton thread and nylon thread are introduced as the substrate of immunochromatography, but the thread itself is loose, uneven, and easy to fall off, so the amount of fixation and the boundary are difficult to control
The gel column immunoreaction using the SPE column as the reactor introduces the detection process into the tube, but the assembly and detection process of this method is complicated

Method used

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  • A kind of preparation method of nano-gold immune chromatography capillary
  • A kind of preparation method of nano-gold immune chromatography capillary
  • A kind of preparation method of nano-gold immune chromatography capillary

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] (1) Handling of capillary :

[0060] Preparation of piranha solution: Mix 95wt% concentrated sulfuric acid and 30wt% hydrogen peroxide at a volume ratio of 4:1. When mixing, slowly add the hydrogen peroxide solution into the concentrated sulfuric acid, and keep stirring to keep the temperature of the mixture below 80°C.

[0061] Quickly immerse the capillary in the above-mentioned hot piranha solution for ultrasonic cleaning for 15 minutes, wash with ultrapure water until neutral, dry in an oven at 105°C for 2 hours, cool down, and then immerse the capillary in sequence with a concentration of 0.8mol / L KOH solution (200mL), Ultrapure water (200mL, replaced twice in the middle), HCl solution (200mL) with a concentration of 0.8mol / L, ultrapure water (200mL, replaced twice in the middle) and acetone (200mL) were ultrasonically cleaned for 15min, and then cleaned at 105 Dry in an oven at ℃ for more than 1 hour to completely remove the moisture.

[0062] (2) Modification...

Embodiment 2

[0068] (1) Handling of capillary :

[0069] Preparation of piranha solution: Mix 98wt% concentrated sulfuric acid and 30wt% hydrogen peroxide at a volume ratio of 3:1. When mixing, slowly add the hydrogen peroxide solution into the concentrated sulfuric acid, and keep stirring to keep the temperature of the mixture below 80°C.

[0070] Quickly immerse the capillary in the above-mentioned hot piranha solution for ultrasonic cleaning for 20 minutes, wash it with ultrapure water until neutral, dry it in an oven at 105°C for 1 hour, and cool it down. Ultrapure water (200mL, replaced twice in the middle), HCl solution with a concentration of 1.2mol / L (200mL), ultrapure water (200mL, replaced twice in the middle) and ethanol (200mL) were ultrasonically cleaned for 15min, and then cleaned at 105 Dry in an oven at ℃ for more than 1 hour to completely remove moisture.

[0071] (2) Modification of capillary :

[0072] Preparation of anhydrous toluene: Add 30g of anhydrous sodium su...

Embodiment 3

[0077] (1) Handling of capillary :

[0078] Preparation of piranha solution: Mix 98wt% concentrated sulfuric acid and 30wt% hydrogen peroxide at a volume ratio of 3:1. When mixing, slowly add the hydrogen peroxide solution into the concentrated sulfuric acid, and keep stirring to keep the temperature of the mixture below 80°C.

[0079] Quickly immerse the capillary in the above-mentioned hot piranha solution for ultrasonic cleaning for 18 minutes, wash with ultrapure water until neutral, dry in an oven at 105°C for 3 hours, and cool down. Ultrasonic cleaning in pure water (200mL, replaced twice in the middle), HCl solution with a concentration of 1mol / L (200mL), ultrapure water (200mL, replaced twice in the middle) and acetone (200mL) for 12min respectively, and then at 105℃ Dry in an oven for more than 1 hour to completely remove moisture.

[0080] (2) Modification of capillary :

[0081] Preparation of anhydrous toluene: Add 20g of anhydrous sodium sulfate to 300mL of t...

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Abstract

The invention discloses a preparation method for nano-gold immunity chromatography capillary. According to the method, a chemical-bond crosslinking method is employed to performing modification on the inner wall of a capillary glass tube, a quality-control zone and a detection zone are fixedly disposed at specific zones of the inner wall of the capillary, so that a novel immunity chromatography capillary is assembled. According to the preparation method, the glass-texture capillary is taken as a base and is more stable compared with capillaries made from cellulose nitrate membranes, paper materials and linear materials; the base material is relatively firm and not easy to fall off, is even more not easily influenced by environmental factors, and is capable of substantially reducing differences between batches and in a batch; and the assembling process is relatively simple and does not need large-scale apparatuses during assembling, and the modification and assembling technologies are simple and easy for mastering.

Description

technical field [0001] The invention relates to the technical field of immunoassay, in particular to a method for preparing a nano-gold immunochromatographic capillary. Background technique [0002] Western blotting, enzyme-linked immunosorbent assay (ELISA), electrochemical sensors and SPR sensors are newly developed rapid detection methods in recent years, which are used in the environment, food and other industries because of their good sensitivity, accuracy, stability and selectivity. Detection of hazard factors in medicine. However, these methods take a long time to operate, and require expensive instruments and professional operating skills, which limit their use in rapid on-site detection, especially in areas where resources are relatively scarce. In the early 1980s, immunocolloidal gold chromatography technology was widely used in the fields of medicine, environment, food testing, agriculture and animal husbandry due to its high sensitivity, strong specificity, simp...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/543
CPCG01N33/5302G01N33/558
Inventor 曹立民杜淑媛隋建新林洪王静雪
Owner OCEAN UNIV OF CHINA
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