Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of 2-keto-l-gulonic acid

A technology of gulonic acid and ketone group is applied in the field of preparation of 2-keto-L-gulonic acid, and achieves the effects of simple operation, high conversion rate of sugar and acid, and reduction of technological difficulty

Active Publication Date: 2017-11-03
ANHUI BBCA FERMENTATION TECH ENG RES
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, little is involved in the effect of the concentration of feeding carbon source on fermentation acid production.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1 Utilizes the technique of concentrated sorbose fermentation to produce 2-keto-L-gulonic acid

[0020] The initial liquid volume in the 50L fermenter is 15L, and the formula of the fermentation medium is: sorbose 25mg / mL, yeast extract 1mg / mL, corn steep liquor 8mg / mL, urea 2mg / mL, magnesium sulfate 0.6mg / mL, calcium carbonate 6mg / mL, prepared with water. Insert the mixed seed solution of Bacillus megaterium and Gluconobacter oxydans at an inoculation amount of 30v / v%. When the residual sugar drops to 20mg / mL, start to add the concentrated sorbose fermented mash. The concentration of sugar is 500mg / L, and the sugar content is controlled to be 20-22mg / mL during the feeding process; at the same time, sodium carbonate is fed during the fermentation process to control the pH at 6.7-6.8. During the fermentation, the initial temperature was 29°C, and after 30 hours, the temperature rose to 30°C, and after 36 hours, the temperature rose to 31°C. The fermentation...

Embodiment 2

[0022] The formula of the sorbose fermentation medium is: sorbitol 260mg / mL, corn steep liquor 2mg / mL, yeast extract 0.3mg / mL, calcium carbonate 0.7mg / mL, prepared with water, and the pH value is natural. Embodiment 2 Utilizes the technique of concentrated sorbose fermentation to produce 2-keto-L-gulonic acid

[0023] In the 50L fermentor, the initial filling volume is 15L, and the fermentation medium formula is the same as embodiment 1, inserts the mixed seed solution of Bacillus megaterium and Gluconobacter oxydans by the inoculation amount of 30v / v%, when residual sugar drops to 20mg / At mL, start to feed the concentrated sorbose fermentation mash, the concentration of sorbose in the mash is 526mg / L, and the sugar content is controlled to be 20-22mg / mL during the feeding process; at the same time, sodium carbonate is added during the fermentation process, Control the pH at 6.7-6.8. During the fermentation, the initial temperature was 29°C, and after 30 hours, the temperatu...

Embodiment 3

[0026] Embodiment 3 Utilizes the technology of concentrated sorbose fermentation to produce 2-keto-L-gulonic acid

[0027] In the 50L fermentor, the initial filling volume is 20L, and the fermentation medium formula is the same as embodiment 1, inserts the mixed seed solution of Bacillus megaterium and Gluconobacter oxydans by the inoculation amount of 30v / v%, when residual sugar drops to 20mg / v% At mL, start to feed the concentrated sorbose fermented mash, the concentration of sorbose in the mash is 550mg / L, the feeding process controls the sugar content to be 20-22mg / mL; at the same time, feed sodium carbonate during the fermentation process, Control the pH at 6.7-6.8. During the fermentation, the initial temperature was 29°C, and after 30 hours, the temperature rose to 30°C, and after 36 hours, the temperature rose to 31°C. The fermentation period is 45 hours, and the final volume of fermentation is 40L. After testing, the content of 2-keto-L-gulonic acid in the fermentat...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
pore sizeaaaaaaaaaa
Login to View More

Abstract

The invention provides a preparation method for 2-keto-L-gulonic acid. The preparation method comprises: firstly concentrating a sorbose fermentation liquid, so as to enable the concentration of sorbose in the liquid to be 500-550 mg / mL, and taking the concentrated liquid as a carbon source to add the concentrated liquid in batches during fermentation of 2-keto-L-gulonic acid. On the one hand, the method helps to avoid inhibition effect of excessively-high sorbitol concentration on growth of gluconbacter melanogenus during fermentation of sorbose, and the concentration of sorbose is improved though the concentrating technology after fermentation is finished; and on the other hand, by improving the concentration of batch-fed sugar, the technology difficulty is reduced, operation is simple, post-extraction is easy, and equipment utilization rate is improved.

Description

technical field [0001] The invention relates to the field of microbial fermentation, in particular to a preparation method of 2-keto-L-gulonic acid. Background technique [0002] At present, domestic production of gulong acid mostly adopts a two-step fermentation method, and the two-step fermentation method usually uses a mixed strain of bacteria for fermentation. In the two-step fermentation process, in order to increase the fermentation yield, the fed-batch process is often used: feeding sorbose and sodium carbonate. The purpose of feeding sodium carbonate is mainly to regulate the pH value, and the reaction product is soluble sodium guronate. Sorbose is used as a fed-in carbon source. When the conversion rate is constant, the amount of sorbose fed-in will directly represent the acid production rate of 2-keto-L-gulonic acid during the two-step fermentation. [0003] The production of sorbose in the industry is mainly obtained by fermenting and transforming sorbitol with ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/60C12R1/11C12R1/07
Inventor 李荣杰杨为华邓远德徐斌穆晓玲
Owner ANHUI BBCA FERMENTATION TECH ENG RES
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com