Method for distinguishing gynogenetic ctenopharyngodon idellus from common ctenopharyngodon idellus

Abstract

The invention provides a method for distinguishing gynogenetic ctenopharyngodon idellus from common ctenopharyngodon idellus, and particularly provides a molecular marking method having the advantages of high polymorphism, genetic stability, high detection success ratio, wide utilization and the like; the molecular marking method is capable of quickly and accurately distinguishing gynogenetic ctenopharyngodon idellus from common ctenopharyngodon idellus. According to the difference in genetic homozygosity, five SSR (Simple Sequent Repeats) which are stable in amplification, clear in bands, polymorphic and great in genetic homozygosity difference in a gynogenetic population and a wild population are screened out from 14 SSR markers. As the genetic homozygosity in the gynogenetic population is greatly improved, the number of alleles amplified in the population by virtue of the SSRs is also reduced. Statistical analysis shows that the actual number of alleles amplified in the common ctenopharyngodon idellus by virtue of the five SSRs is 8-10 while the number of alleles amplified in the gynogenetic ctenopharyngodon idellus is 5-7, so that the gynogenetic ctenopharyngodon idellus and the common ctenopharyngodon idellus can be distinguished from each other by comparing the number of alleles, and the theoretical value of the accuracy rate can be 99.92%.

Description

【Technical field】 [0001] The invention belongs to the technical field of molecular markers and relates to a molecular marker method for identifying gynogenetic grass carp and common grass carp. 【Background technique】 [0002] Grass carp (Ctenopharyngodon idellus) belongs to Cyprinidae, Cyprinidae, and the genus Grass Carp, also known as grass carp, and is one of the important freshwater economically farmed fish in my country. Grass carp is widely distributed in my country, and its annual output accounts for about 20% of the total freshwater fish output in my country. In recent years, grass carp germplasm resources have been seriously degraded and diseases are frequent, which has brought huge losses to the grass carp aquaculture industry. However, due to the long period required for the reproduction of grass carp (generally 4-5 years for sexual maturity), the traditional methods for germplasm improvement and breeding of excellent varieties of grass carp are progressing slowl...

AI Technical Summary

Problems solved by technology

However, gynogenetic grass carp is indistinguishable from common grass carp in terms of morphology and physiology, and is easily confused in the actual breeding and conservation process. A molecular biological method that can distinguish gynogenetic grass carp from common grass carp was established by using microsatellite molecular markers Undoubtedly an effective way to solve this problem

[0003] At present, in the aspect of identifying gynogenetic grass carp, some scholars have used isozyme and RAPD marker technology to study the genetic diversity, homozygosity and loss of polymorphic sites of gynogenetic grass carp. However, isozymes are For gene expression products, the detection sites are limited, and it is not easy to distinguish varieties with close relationship or complex genetic basis; the stability and repeatability of RAPD markers are poor, and it is difficult to meet actual needs

In comparison, microsatellite (SSR) marker technology has been widely used in animals and plants due to its co-dominant inheritance, a large number of detection sites, high polymorphism, stable inheritance, and not affected by environmental conditions. There are no reports on species identification and other studies, and the use of microsatellite molecular markers to identify gynogenetic populations and common populations in the Yangtze River system

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