Glycosylation modification method of rice residue protein

A technology of rice dregs protein and glycosylation, which is applied in the field of glycosylation modification of rice dregs protein, can solve the problems of less research, achieve the effect of reducing blood fat and blood pressure, expanding application fields, and good antioxidant activity

Active Publication Date: 2014-08-27
长沙飞创生物技术有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Domestic work in this area started relatively late, and most of the research on improving the function of hydrophobic vegetable protein focused on soybean protein. For grain protein, especially rice dregs protein, which has more urgent needs for solubilization and functional properties improvement, its less research

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Wash the rice dregs twice with high-temperature water at 90°C, add high-temperature-resistant α-amylase to the rice dregs at a rate of 120 U / g, and enzymatically hydrolyze at pH 4.5 and 90°C for 1 hour to degrade residual starch; after inactivating the enzyme, press Add alkaline protease at 1200 U / g, pre-enzyme hydrolyze for 2 hours at pH 8.0 and 45°C, then adjust the pH of the reaction system to 12 with dilute alkali (NaOH with a mass percentage concentration of 1%), and then at 45°C, liquid-solid Under the condition of ratio 10:1, the rice dregs protein was obtained by alkaline extraction for 2 h. Dissolve 1 g of rice dregs protein in 100 mL of distilled water to make a solution with a pH value of 11 (using 1 mol / L NaOH and 1 mol / L HCL), stir in a water bath at 55 °C with a magnetic stirrer for 4 h, and cool to room temperature for later use; Polysaccharides were added to the prepared rice dregs protein solution, the mass ratio of polysaccharides to rice dregs protein...

Embodiment 2

[0020] Wash the rice dregs twice with high-temperature water at 90°C, add high-temperature-resistant α-amylase to the rice dregs at a rate of 120 U / g, and enzymatically hydrolyze at pH 4.5 and 90°C for 1 hour to degrade residual starch; after inactivating the enzyme, press Add alkaline protease at 1200 U / g, pre-enzyme hydrolyze for 2 hours at pH 8.0 and 45°C, then adjust the pH of the reaction system to 12 with dilute alkali (NaOH with a mass percentage concentration of 1%), and then at 45°C, liquid-solid Under the condition of ratio 10:1, the rice dregs protein was obtained by alkaline extraction for 2 h. Dissolve 1 g of rice dregs protein in 100 mL of distilled water to make a solution with a pH value of 11 (using 1 mol / L NaOH and 1 mol / L HCL), stir for 4 hours in a water bath at 55 ° C with a magnetic stirrer, and cool to room temperature for later use; The polysaccharide was added to the prepared rice dregs protein solution, the mass ratio of the polysaccharide to the rice...

Embodiment 3

[0022] Wash the raw rice dregs twice at 90°C with high temperature water, add high-temperature-resistant α-amylase at 120 U / g, enzymolyze at pH 4.5 and 90°C for 1 hour, and degrade residual starch; after inactivating the enzyme, add 1200 U / g g Add alkaline protease, at pH 8.0, at 45°C, pre-enzyme hydrolyze for 2 hours, then use dilute alkali (NaOH with a mass percentage concentration of 1%) to adjust the pH of the reaction system to 12, at 45°C, the liquid-solid ratio is 10:1 Under these conditions, alkaline extraction was carried out for 2 h to obtain protein from rice dregs. Dissolve 1 g of rice dregs protein in 100 mL of distilled water to make a solution with a pH value of 11 (with 1 mol / L NaOH and 1 mol / L HCL), stir in a water bath at 55 °C for 4 h with a magnetic stirrer, and cool to room temperature for later use; Lentinan was added to the prepared rice dregs protein solution, the mass ratio of polysaccharide to rice dregs protein was 5:1, the pH was adjusted to 11, mag...

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PUM

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Abstract

The invention discloses a glycosylation modification method of rice residue protein. According to the method, the Maillard reaction serves as a theoretical basis, glycosylation wet-process modification is carried out on the soluble rice residue protein extracted with rice residues as raw materials through different polysaccharide, wherein the rice residues are secondary products of deep processing of rice, the solubleness, foamability and emulsibility of the protein are improved, the modified rice residue protein has the good antioxidant activity and has the effects of regulating the immune, regulating the blood sugar level, resisting a tumor, delaying senescence and the like, the scientific basis and scientific research basis are provided for further exploring the functions of food-borne protein, a new path for further development and utilization of hydrophobicity plant protein such as rice protein is expected to be obtained, and the glycosylation modification method has the important theoretical value and application prospects.

Description

technical field [0001] The invention belongs to the research field of modification of hydrophobic vegetable protein, and in particular relates to a glycosylation modification method of rice dregs protein. Background technique [0002] Rice is the largest grain crop in my country, and its total output has long ranked first in the world. For a long time, the deep processing of rice production and its by-products has been highly concerned by food science researchers. At present, rice dregs are mainly used as animal feed, and rice dregs protein extracted from rice dregs is widely used in the food industry. The development and utilization of rice dregs protein is based on the research and comprehensive utilization of enriched rice processing products and rational utilization of rice processing by-products. 75%-80% of rice dregs protein is gluten, and its amino acid composition is basically the same as that of rice protein. gluten [5] Its amino acid composition is balanced and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/107
Inventor 梁盈林亲录鲁倩刘颖吴伟杨涛吴跃
Owner 长沙飞创生物技术有限责任公司
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