PCR-HRM primer and method for quickly distinguishing canine parvoviruses of different genotypes
A canine parvovirus, genotype technology, applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc., can solve the problems of complex operation, limited application, high cost, and achieve good repeatability, The effect of good degeneracy and low cost
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0061] (1) PCR-HRM primers
[0062] After screening a large number of designed primers, it was found that the base sequences of the primers, SEQ ID NO: 1 and SEQ ID NO: 2, have the best effect on the PCR-HRM method for distinguishing different genotypes of canine parvovirus, and the base sequences are as follows Show.
[0063] P1 : 5'-CCAGAAGGAGATTGGATTCA-3' (SEQ ID NO: 1),
[0064] P2: 5'-TTAATGCAGTTAAAGGACCAT-3' (SEQ ID NO: 2).
[0065] Among them, the primer P1 is on the 4014-4033 position of the canine parvovirus VP2 gene, and the primer P2 is on the 4138-4158 position of the canine parvovirus VP2 gene (the accession number of this gene in Genbank is M38245).
[0066] (2) Construction of standard plasmid samples and PCR-HRM analysis
[0067] 1) Extraction of canine parvovirus DNA:
[0068] Canine parvovirus DNA in disease samples was extracted with MiniBEST Viral RNA / DNA Extraction Kit Ver.4.0. Disease samples can be whole blood, feces, rectal swabs and other samp...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com