Cultivation method of fordia cauliflora hemsl tissue culture seedlings
A cultivation method and technology for dried pinto beans, which are applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems that seedlings are susceptible to diseases and insect pests, limit the utilization of dried pinto beans as medicinal plants, and are difficult to mass-produce, and achieve the goal of overcoming the problems of seedlings. It is not easy to germinate, realizes mass production, and has the effect of high survival rate
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Embodiment 1
[0024] 1) In April, choose mature, bright in color, full, plump dried Pinto bean seeds and wash them with running water for 20 minutes, soak them in 70% (volume) ethanol for 20 seconds on the ultra-clean workbench, wash them with sterile water for 3 times, Concentration is 0.3% (mass) after immersing in the mercuric chloride of 8min, take out, inoculate on the aseptic sprout culture medium after washing 3 times with distilled water, in every 50ml Erlenmeyer flask, 5~8 seeds, after light culture 23d, Germinated dried pinto bean seedlings were obtained; wherein, the formula of the aseptic sprout culture medium was: 1 / 2MS+GA31.5mg / L+sucrose 30g / L+NAA2mg / L+agar 5g / L, the pH was 5.8; the light culture conditions were : light intensity 1500lx, light time 18h / d, temperature 26-28°C; dark treatment 6h / d, temperature 16-18°C, replace the medium every 6 days;
[0025] 2) Select disease-free and vigorously grown dried pinto bean seedlings, cut out the stems with leaves and place them in ...
Embodiment 2
[0031]1) Select a robust 5-year-old dried flower bean mother plant, and artificially pollinate it when it blooms. From October to December, harvest the dried flower bean pods that have been artificially pollinated and developed to maturity but have not yet cracked, and store them according to conventional methods ,spare;
[0032] 2) In April of the next year, choose the bright, substantial and plump dried Pinto bean seeds and wash them with running water for 20 minutes, soak them in 70% (volume) ethanol for 20 seconds on the ultra-clean workbench, wash them with sterile water for 3 times, and wash them with sterile water at a concentration of After soaking in 0.1% (mass) mercuric chloride for 10 minutes, take it out, rinse it with distilled water for 3 times, and inoculate it on the aseptic sprout culture medium, put 10 seeds in each 50ml triangular flask, and cultivate it under light for 25 days to obtain germinated dry flowers Bean seedlings; wherein, the formula of the ster...
Embodiment 3
[0039] 1) In April, select mature, bright in color, full, plump dried Pinto bean seeds to wash with running water for 30 minutes, soak them in 75% (volume) ethanol for 20 seconds on an ultra-clean workbench, wash them with sterile water for 3 times, Concentration is 0.1% (mass) after immersing in the mercuric chloride of 0.1% (mass) after soaking 10min, take out, inoculate on the aseptic sprout culture medium after washing with distilled water 3 times, in each 50ml Erlenmeyer flask, 8 seeds, after light culture 20d, obtain germination. dry pinto bean seedlings; wherein, the formula of the aseptic sprout culture medium is: 1 / 2MS+GA31.0mg / L+sucrose 25g / L+NAA3mg / L+agar 4g / L, pH is 6.0; light culture conditions are: light The intensity is 1800lx, the light time is 20h / d, and the temperature is 25-27°C; the dark treatment is 4h / d, the temperature is 17-19°C, and the medium is replaced every 5 days;
[0040] 2) Select disease-free and vigorously grown dried pinto bean seedlings, cut...
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