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Application of hepatocyte growth factor gene in preparation of medicament for preventing and/or treating pain

A technology of hepatocyte growth factor and gene, applied in the field of application of hepatocyte growth factor gene in the preparation of drugs for the prevention and/or treatment of pain, which can solve the problem of less selectivity and achieve the effect of broad application prospects

Inactive Publication Date: 2014-09-03
INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, although there are opioid, morphine-type analgesics and non-steroidal anti-inflammatory analgesics clinically, these drugs still provide relatively few options for patients. Therefore, it is still necessary to develop new treatments for pain. drug

Method used

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  • Application of hepatocyte growth factor gene in preparation of medicament for preventing and/or treating pain
  • Application of hepatocyte growth factor gene in preparation of medicament for preventing and/or treating pain
  • Application of hepatocyte growth factor gene in preparation of medicament for preventing and/or treating pain

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Obtaining of recombinant plasmid vector carrying human hepatocyte growth factor gene

[0039] 1. Cell preparation

[0040] The self-constructed plasmid carrying the human hepatocyte growth factor gene contains the CMV promoter, the human hepatocyte growth factor gene, and the linear expression cassette of the polyA tail. The host bacteria is Escherichia coli DH5α, which is fermented according to the conventional method, and the bacteria are obtained after centrifugation, and stored at -20°C for later use.

[0041] 2. Alkaline lysis and broken cells to extract plasmid DNA

[0042] According to the method and device for lysing cells and extracting plasmid DNA disclosed in the patent No. ZL201110089982.2 (authorized announcement date is August 28, 2013, the patent name is alkaline lysis system and combined system for preparing plasmid DNA) and the extraction of plasmid DNA , where the improvement steps are as follows:

[0043] 1. The clarified alkali lysate obtained by ...

Embodiment 2

[0051] Analgesic effect of human hepatocyte growth factor gene on acute pain induced by skin and muscle incision in SD rats

[0052] 1. Animal model

[0053] SPF grade male SD rats weighing 210-230 g were anesthetized by intraperitoneal injection of pentobarbital sodium (45 mg / kg), and the left hind feet of the rats were disinfected, and lengthened from the proximal 0.5 cm of the sole of the paw to the toe using a No. 11 scalpel blade. A longitudinal incision of about 1.0 cm was made. After the skin was cut, ophthalmic tweezers were used to pick up the muscle of the paw floor and bluntly separate it longitudinally, but the start, stop and attachment of the muscle were kept intact. After pressing to stop the bleeding, the skin was sutured with 5-0 nylon suture for two stitches. The requirement for suturing is that the incision skin cannot be overlapped, turned inward, or split. After the operation, 0.1ml of gentamicin was injected.

[0054] This model can induce spontaneous ...

Embodiment 3

[0067] Analgesic effect of human hepatocyte growth factor gene on central hypersensitivity pain induced by skin / muscle incision stretch in SD rats

[0068] 1. Animal model

[0069] SPF grade male SD rats weighing 210-230 g were anesthetized by intraperitoneal injection of pentobarbital sodium (45 mg / kg), fixed in the supine position, and made a 1.5-2 cm long skin incision at the inner side of the saphenous vein in the middle of the right hind leg, exposing the leg Then make a 7-10mm long incision in the superficial muscle (gracis muscle), and bluntly separate the superficial muscle. After seeing the white adductor fascia, insert a micro retractor into the incision. Place the tip of the microscopic retractor under the superficial muscle, pull the skin and muscle to 2cm, expose the adductor fascia, and continue pulling for 1 hour. During stretching, the saphenous nerve is stretched and displaced by the retractor, but it is not compressed by hard objects such as bones because it...

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Abstract

The invention discloses an application of a hepatocyte growth factor gene in preparation of a medicament for preventing and / or treating pain. The paint caused by skin damages, the pain caused by muscle damages or neuropathic pain caused by nerve injuries can be significantly relieved by the application of the medicament. The application disclosed by the invention has an important application prospect and practical significance.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to the application of a hepatocyte growth factor gene in the preparation of drugs for preventing and / or treating pain. Background technique [0002] Pain is one of the most common complications of diseases, and most hospitals now treat pain as the fifth vital sign. Pain mainly includes acute pain and chronic pain, and chronic pain includes inflammatory and neuropathic pain and cancer pain. Inflammatory pain is related to the immune response to tissue damage and infection, and is caused by a variety of diseases, such as rheumatoid or arthritis, pancreatitis, inflammatory bowel disease, interstitial cystitis, or chronic pelvic pain syndrome of the bladder; Neuropathic pain is pain caused by primary lesions or nervous system dysfunction, resulting from damage to nerve tissue. Postoperative pain is a complex physiological and psychological response of the human body to tissue damage and rep...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K48/00A61P29/00
Inventor 张庆林胡春生程晓晨卢育新吴祖泽
Owner INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA
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