Original ecological biological wall-breaking technology of ganoderma lucidum spore powder

A technology of Ganoderma lucidum spores and spore powder, which is applied in the directions of microorganisms, microbial dissolution, and microorganism-based methods, can solve the problems of short shelf life, poor preservation, and difficulty in removing enzymes or bacteria, and achieves simple preservation process and short shelf life. , The effect of low production cost

Inactive Publication Date: 2014-09-03
信芝(厦门)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Biological method: including enzymatic hydrolysis and bacteriolysis. These methods have the advantages of low energy consumption and good wall breaking effect. The disadvantage is that it is very difficult to remove enzymes or bacteria in the product
[0005] Chemical method: The advantages of this method are similar to those of the biological method, but the disadvantage is that it often leads to the denaturation of active ingredients, high harmful residues in the product, and it is difficult to guarantee the effectiveness and safety of the product
[0006] Physical mechanical method: the advantage is that it is simple and easy to implement, but the disadvantage is that the spore oil spills out and is not easy to preserve, the shelf life is short, and the operation cost of complex mechanical equipment is high

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0032] 1) Use PDA medium to separate and preserve the female parental strain of Ganoderma lucidum (3-6% by weight of the strain to inoculate the medium, after growing up, use the newly grown strain to inoculate the medium again under the same conditions, inoculate three times in total), add 3% by weight The PDA medium slant medium of peptone continues to expand the required number of female slant bacteria;

[0033] 2) Remove impurities such as sand and soil in the spore powder by filtration separation method (filter paper pore size is 400 mesh), and extract high-purity spore powder;

[0034] 3) Fill the high-purity spore powder (obtained in step 2) with a biological culture bottle, and control the water content of the spore powder at 60% (higher than 60%, too thick, mycelium growth is not good, and less than 60% is too dry, not There will be hyphae), perform high-pressure sterilization (124-126 degrees, 2 half an hour each), after cooling at 25 degrees Celsius, insert the moth...

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Abstract

The invention discloses an original ecological biological wall-breaking technology of ganoderma lucidum spore powder. The technology comprises the following steps: separating and storing the female parent strains of ganoderma lucidum and further increasing the female parent cant strains in required numbers via a peptone cant culture medium; removing impurities from the spore powder by using a separation method and purifying so as to obtain the high-purity spore powder; filling the high-purity spore powder in a biological culture bottle obtained by the step 1, carrying out autoclaved sterilization by controlling the water content of the spore powder at 60% and grafting the female parent strains into the spore powder after cooling to 25 DEG C; standing at 25 DEG C to 28 DEG C and culturing for 20 to 25 days so as to realize 100% of the wall breaking of the spore powder; and taking out the spore powder with the wall breaking, carrying out infrared sterilization at a high temperature and drying. According to the technology, the wall-breaking decomposition is carried out by utilizing the female parent strains, so that the wall-breaking ratio reaches 100%, i.e., the residue problem of biological decomposition enzymes is solved; the wall-breaking culturing is carried out in a standing manner, so that the integrity of spores is maintained; the spores cannot be decomposed unless meeting water, so that the problem that the storage time of the spore powder is short due to the physical and mechanical wall breaking is solved. Thus, the quality guarantee time of the spore powder is greatly prolonged.

Description

technical field [0001] The invention relates to the field of ganoderma wall breaking. Specifically, it relates to a technique for breaking the original ecological biological wall of ganoderma lucidum spore powder. Background technique [0002] At present, Ganoderma lucidum has been widely used in medicine, food beauty and other products. Ganoderma lucidum spore powder is the essence of Ganoderma lucidum, and it is also one of the popular drugs for anti-cancer and enhancing body immunity in recent years. Ganoderma lucidum spores have a layer and a double-layer outer wall of chitin structure that is difficult to be digested by human gastric acid. Most of the active reports indicate that the main active substances of Ganoderma lucidum are concentrated in the spores of Ganoderma lucidum. The active ingredients and medicines contained in them are of high edible value. The fruiting body of Ganoderma lucidum is several times larger. Only by opening this layer of outer wall can the...

Claims

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Application Information

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IPC IPC(8): C12N1/06C12R1/645
Inventor 陈彦卿陈金龙
Owner 信芝(厦门)生物科技有限公司
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