Method for efficiently extracting full-component grateloupia filicina extract by utilizing two-enzyme method

A technology of centipede algae and full ingredients, which is applied in the field of efficient extraction of full-component centipede algae extract, can solve the problems of difficult control of addition conditions, loss of active ingredients, cumbersome extraction methods, etc., to save labor and material resources, reduce addition amount, and enhance immunity force effect

Inactive Publication Date: 2014-09-17
GUANGDONG SHAOHE PEARL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, existing methods are difficult to extract components such as cysteine, histidine, proline, tryptophan, zinc, iron, etc., resulting in the loss of extracted active ingredients.
In addition, in the prior art, hydrochloric acid and sulfuric acid are often used to adjust th

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0033] Example one: Weigh 2 kg of centipede algae, add 70 kg of deionized water, stir well, add 7.2 g of anhydrous sodium carbonate, and adjust the pH to 7. Add 200g of agarase, stir well, and heat in the reaction pot at 25°C for 8h. Add 144g potassium sorbate and 23mL lactic acid, stir well, and adjust the pH to 5.0. Add 122g of cellulase, stir well, and heat in the reaction pot at 50°C for 12 hours. After the reaction, the enzymatic hydrolysate was centrifuged (the residue is 576g). Add 22 g of anhydrous sodium carbonate to the centrifugal clear liquid, adjust the pH to 7, and heat to 100°C to sterilize and kill the enzyme. The resulting solution is the centipede algae extract with all ingredients, let cool for later use. The extraction rate of the all-component centipede algae extract was 27.74%, and the yield was 92.47%.

Example Embodiment

[0034] Embodiment two: Weigh 2 kg of centipede algae, add 70 kg of deionized water, stir evenly, add 8.4 g of anhydrous sodium carbonate, and adjust the pH to 7.5. Add 240g of agarase, stir well, and heat in the reaction pot at 25°C for 8 hours. Add 144g potassium sorbate and 25mL lactic acid, stir well, and adjust the pH to 5.1. Add 140g of cellulase, stir well, and heat in the reaction pot at 52°C for 12 hours. After the reaction, centrifuge the enzymatic hydrolysate (the residue is 920 g). Add 20g of anhydrous sodium carbonate to the centrifugal clear liquid, adjust the pH to 7, and heat to 100°C to sterilize and kill the enzyme. The resulting solution is the centipede algae extract with all ingredients, let cool for later use. The extraction rate of the all-component centipede algae extract was 26.83%, and the yield was 89.43%.

Example Embodiment

[0035] Embodiment three: Weigh 2 kg of centipede algae, add 70 kg of deionized water, stir well, add 7.2 g of anhydrous sodium carbonate, and adjust the pH to 7. Add 144g of agarase, stir well, and heat in the reaction pot at 25°C for 8 hours. Add 144mL potassium sorbate and 23mL lactic acid, stir well, and adjust the pH to 5.0. Add 72g of cellulase, stir well, and heat in the reaction pot at 50°C for 12 hours. After the reaction, the enzymatic hydrolysate was centrifuged (the residue is 1728g). Add 22 g of anhydrous sodium carbonate to the centrifugal clear liquid, adjust the pH to 7, and heat to 100°C to sterilize and kill the enzyme. The resulting solution is the centipede algae extract with all ingredients, let cool for later use. The extraction rate of the all-component centipede algae extract was 24.53%, and the yield was 81.77%.

[0036] In this example, the addition of agarase and cellulase decreased, resulting in incomplete destruction of the cell wall, increased ins...

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Abstract

The invention provides a method for efficiently extracting full-component grateloupia filicina extract by utilizing a two-enzyme method. The method comprises the following steps: adding water into grateloupia filicina, and uniformly stirring to form a backing material; regulating the pH to 6.5-7.5 by adding anhydrous sodium carbonate; weighing agarase which is 0.25-0.39 percent of the weight of the backing material, dissolving with water, and performing enzymolysis at 20-35 DEG C for 7-9 hours in the pH value regulated backing material; adding lactic acid and potassium sorbate into the enzymatic hydrolysate, uniformly stirring, and controlling the pH to be 4.8-5.2; weighing cellulase which is 0.17-0.22 percent of the weight of the backing material, dissolving with water, and performing enzymolysis at 45-55 DEG C for 11-14 hours in the previous liquid; centrifuging or performing pump filtration after the reaction is completed to obtain a clear liquid; and adding anhydrous sodium carbonate into the clear liquid to regulate the pH to be 6.5-7.5, heating to perform sterilization and enzyme deactivation to obtain a liquid which is the full-component grateloupia filicina extract. According to the method, the purity of the full-component grateloupia filicina extract can be guaranteed, and the extraction rate of effective components of grateloupia filicina can be effectively improved.

Description

technical field [0001] The invention relates to a method for extracting seaweed, in particular to a method for efficiently extracting the whole component centipede algae extract by a double-enzyme method. Background technique [0002] At present, my country is the main seaweed producing country in the world, harvesting more than 1.5 million tons of various seaweeds (according to dry products) every year. These seaweeds are used to produce alginates and esters, agar, carrageenan, and are widely used in processed food, food additives, pharmaceuticals, chemical products and other industries. Studies have shown that the active substances of seaweed include seaweed polysaccharides, seaweed oligosaccharides, small molecular peptides and various vitamins and trace elements, which can improve blood circulation in the brain, enhance myocardial function, and prevent cardiovascular diseases. It has special effects on cerebral thrombosis, etc., and at the same time can enhance human im...

Claims

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Application Information

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IPC IPC(8): C08B37/04
Inventor 庄铠燕林展新谢绍河
Owner GUANGDONG SHAOHE PEARL
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