Method for efficiently extracting full-component grateloupia filicina extract by utilizing two-enzyme method

Abstract

The invention provides a method for efficiently extracting full-component grateloupia filicina extract by utilizing a two-enzyme method. The method comprises the following steps: adding water into grateloupia filicina, and uniformly stirring to form a backing material; regulating the pH to 6.5-7.5 by adding anhydrous sodium carbonate; weighing agarase which is 0.25-0.39 percent of the weight of the backing material, dissolving with water, and performing enzymolysis at 20-35 DEG C for 7-9 hours in the pH value regulated backing material; adding lactic acid and potassium sorbate into the enzymatic hydrolysate, uniformly stirring, and controlling the pH to be 4.8-5.2; weighing cellulase which is 0.17-0.22 percent of the weight of the backing material, dissolving with water, and performing enzymolysis at 45-55 DEG C for 11-14 hours in the previous liquid; centrifuging or performing pump filtration after the reaction is completed to obtain a clear liquid; and adding anhydrous sodium carbonate into the clear liquid to regulate the pH to be 6.5-7.5, heating to perform sterilization and enzyme deactivation to obtain a liquid which is the full-component grateloupia filicina extract. According to the method, the purity of the full-component grateloupia filicina extract can be guaranteed, and the extraction rate of effective components of grateloupia filicina can be effectively improved.

Description

technical field [0001] The invention relates to a method for extracting seaweed, in particular to a method for efficiently extracting the whole component centipede algae extract by a double-enzyme method. Background technique [0002] At present, my country is the main seaweed producing country in the world, harvesting more than 1.5 million tons of various seaweeds (according to dry products) every year. These seaweeds are used to produce alginates and esters, agar, carrageenan, and are widely used in processed food, food additives, pharmaceuticals, chemical products and other industries. Studies have shown that the active substances of seaweed include seaweed polysaccharides, seaweed oligosaccharides, small molecular peptides and various vitamins and trace elements, which can improve blood circulation in the brain, enhance myocardial function, and prevent cardiovascular diseases. It has special effects on cerebral thrombosis, etc., and at the same time can enhance human im...

AI Technical Summary

Problems solved by technology

In addition, existing methods are difficult to extract components such as cysteine, histidine, proline, tryptophan, zinc, iron, etc., resulting in the loss of extracted active ingredients.

In addition, in the prior art, hydrochloric acid and sulfuric acid are often used to adjust the pH. Hydrochloric acid and sulfuric acid are highly acidic, highly corrosive, difficult to control the addition conditions, difficult to operate during the implementation process, and the extraction method is cumbersome, and the safety of the extracts for food and cosmetics is low.