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Sesquiterpene compound with antitumor activity and preparation method thereof

A technology of sesquiterpenoids and compounds, applied in the field of sesquiterpenoids and their preparation, can solve problems such as drugs that have not yet been seen, and achieve the effects of novel structure, simple preparation method, and strong anti-tumor activity

Inactive Publication Date: 2015-10-14
FUJIAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have found that the sesquiterpenoids have anti-tumor activity, and there is no report on the inhibitory activity of the sesquiterpenoids on tumor cells, so there are no related drugs on the market

Method used

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  • Sesquiterpene compound with antitumor activity and preparation method thereof
  • Sesquiterpene compound with antitumor activity and preparation method thereof
  • Sesquiterpene compound with antitumor activity and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Prepare solid PDA medium (15 L per liter of water) and divide into 750 90 mm glass petri dishes, each containing about 20 mL of medium, insert activated wild abalone mushroom ( Pleurotus cystidiosus ) ZYB2013 (preserved in the China Center for Type Culture Collection on June 15, 2014, with the preservation number CCTCC M 2014256) mycelium block, cultured in a constant temperature incubator at 28 °C for 43 days. After cultivation, chop wild abalone mushroom mycelium together with the culture medium, put it in a faucet bottle, add organic solvent (ethyl acetate: methanol: acetic acid volume ratio = 80:15:5) for leaching 6 times, and collect the extract , concentrated under reduced pressure at 40°C to a paste, extracted 6 times with pure water and ethyl acetate 1:1, dehydrated the ethyl acetate phase with anhydrous sodium sulfate, concentrated under reduced pressure at 40°C to a paste, The ethyl acetate crude extract (4.4057 g) was obtained.

[0023] The 4.4057 g ethyl ac...

Embodiment 2

[0034] The inhibitory effects of the compounds on prostate cancer cells DU-145, C42B and LNCaP were determined by MTT method. The cultured prostate cancer cells DU-145, C42B and LNCaP were made into a single cell suspension, counted with a cell plate and diluted to a cell concentration of 6×10 4 individual / mL. Cells were seeded in 96-well plates, 80 μL per well. In addition, 2 wells without cells and only 80 μL of culture medium [Dulbecco’s modified Eagle’s media (DMEM, Gibco, USA) + 10% calf serum] were set as blank control wells for instrument zeroing. Set at 37°C, 5% CO 2 Incubate in an incubator for 24 h, and then add 20 μL of the sample diluted with culture medium. At the same time, add 20 μL of cisplatin to the positive control wells, and add 20 μL of culture solution to the negative control wells and blank control wells. Continue to culture for 72 hours, and add 10 μL of 5 mg / mL MTT to each well. React at 37°C for 3 h, add 100 μL of 10% SDS-0.01mol / L HCl to each we...

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Abstract

The invention relates to sesquiterpene compounds with antitumor activity and a preparation method of the sesquiterpene compounds, belonging to the field of biological medicines. The sesquiterpene compounds have the structural formula as the specification. The compounds are obtained from (Pleurotus cystidiosus) ZYB2013, and the sesquiterpene compounds are used as antitumor drugs or tumor cell proliferation inhibiting drugs. The sesquiterpene compounds provided by the invention are novel in structure and strong in antitumor activity. The sesquiterpene compounds are found to have a relatively strong inhibiting effect for prostate cancer cells DU-145, C42B and LNCaP through measuring the antitumor activities of the compounds. A result shows that the sesquiterpene compounds have antitumor activity so as to be applied to preparation of antitumor drugs or other biological activity primers.

Description

technical field [0001] The invention relates to a sesquiterpene compound with antitumor activity and a preparation method thereof, belonging to the field of biomedicine. Background technique [0002] Sesquiterpenes are natural terpenoids with 15 carbon atoms in the molecule. Sesquiterpenoids are widely distributed and are most abundant in magnoliales, rutales, cornales and asterales. In plants, it often exists in volatile oils in the form of alcohols, ketones, lactones, etc., and is the main component of high boiling point parts in volatile oils. Most of them have strong aroma and biological activity, and are important raw materials for medicine, food and cosmetic industries. [0003] The inventor has studied and learned that wild abalone mushroom ( Pleurotus cystidiosus ) ZYB2013 (preserved in the China Center for Type Culture Collection on June 15, 2014, with the preservation number CCTCC M 2014256), the hyphae in the early stage of solid culture are white, and the myce...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D307/86A61K31/343A61P35/00C12P17/04C12R1/645
CPCC07D307/86C12P17/04
Inventor 郑永标王继峰庞海月
Owner FUJIAN NORMAL UNIV
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