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Preparation and application of a modified hydroxyapatite microcapsule biomaterial carrier

A technology of hydroxyapatite and biomaterials, applied in the direction of medical preparations of non-active ingredients, drug delivery, pharmaceutical formulations, etc., can solve the problems of low load capacity, uncontrollable release process, easy dissipation, etc., and achieve low cost , active ingredient isolation, type and dosage of less effect

Active Publication Date: 2016-08-17
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when nanoparticles are directly used to load biological materials, there are disadvantages such as relatively small loading capacity, easy dissipation of loaded biological materials directly exposed to the environment, and difficult control of the release process.

Method used

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  • Preparation and application of a modified hydroxyapatite microcapsule biomaterial carrier
  • Preparation and application of a modified hydroxyapatite microcapsule biomaterial carrier
  • Preparation and application of a modified hydroxyapatite microcapsule biomaterial carrier

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Embodiment 1 (product preparation):

[0051] A modified hydroxyapatite microcapsule biological material carrier, the preparation steps of which are as follows:

[0052] (1) Modification of hydroxyapatite nanoparticles:

[0053] 0.500g (4.98×10 -4 mol) hydroxyapatite nanoparticles were placed in a round bottom flask, then 0.5mmol of dibutyl phosphate was added, and then 50ml of acetone was added to disperse to form a reaction system. The concentration of dibutyl phosphate in the reaction system was 0.01mol / L. Under the condition of 57°C water bath, stir and reflux for 5 hours; after the reflux, the product is separated by suction filtration, and then the product is washed 8 times with acetone by suction filtration; the washed product nanoparticles are collected and dried in a vacuum oven at 60°C for 24 Hour.

[0054] (2) Preparation of modified hydroxyapatite microcapsule biomaterial carrier:

[0055] Put 20 mg of the dried product nanoparticles obtained in step (1) ...

Embodiment 2

[0056] Embodiment 2 (product preparation):

[0057] A modified hydroxyapatite microcapsule biological material carrier, the preparation steps of which are as follows:

[0058] (1) Modification of hydroxyapatite nanoparticles:

[0059] 0.500g (4.98×10 -4 mol) hydroxyapatite nanoparticles were placed in a round bottom flask, then 0.5mmol of dibutyl phosphate was added, and then 50ml of acetone was added to disperse to form a reaction system. The concentration of dibutyl phosphate in the reaction system was 0.01mol / L. Under the condition of a water bath at 57°C, stir and reflux for 5 hours; after the reflux, separate the product by suction filtration, and then wash the product 8 times with acetone by suction filtration. The product nanoparticles after washing were collected and dried in a vacuum oven at 60° C. for 24 hours.

[0060] (2) Preparation of modified hydroxyapatite microcapsule biomaterial carrier:

[0061] Put 20 mg of the dried product nanoparticles obtained in st...

Embodiment 3

[0062] Embodiment 3 (application): hydroxyapatite microcapsule material is used as the carrier application experiment of fluorescent dye calcein

[0063] Choose to use 10 -5 The mol / l calcein physiological saline solution is used as the water phase, and the edible oil is used as the oil phase. Calcein is a small molecule water-soluble fluorescein with stable properties and can be easily detected by fluorescence spectrophotometry or ultraviolet-visible spectrophotometry. It is often used as a model drug for drug carriers.

[0064] Use 1ml of edible oil as the oil phase, add 20 mg of the modified hydroxyapatite nanoparticles prepared in step (1) of Example 1, use ultrasonic waves to fully disperse it in the oil phase, and then add 100 μl of 10 -5 mol / l calcein saline solution, shake vigorously to form emulsion. Observation with an optical microscope: it can be seen that the microcapsule material is formed with calcein saline solution as the core material and modified hydroxyap...

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Abstract

The invention belongs to the technical field of preparation and application of biocompatible carrier materials, and specifically discloses the preparation and application of a modified hydroxyapatite microcapsule biomaterial carrier. The hydroxyapatite nanoparticles and dibutyl phosphate were placed in acetone, so that n-butyl group was modified to the surface of the nanoparticles, and the surface of the nanoparticles had both hydroxyl groups and n-butyl groups, so as to obtain a hydrophilic / hydrophobic amphiphilic Nanoparticles after permanent surface modification; put this nanoparticle in the water phase or oil phase, then add the oil phase or water phase as the core material, vibrate violently, and obtain the surface modified nanoparticle as the membrane material through self-assembly microcapsule material with water / oil interfacial stability. The microcapsule material has a large load-carrying rate, active ingredient isolation and specified release capacity, etc., and the simple water-soluble or fat-soluble biological material is dissolved in the core material, and the microcapsule carrier material can be conveniently used as the biological material. efficient carrier for practical application.

Description

technical field [0001] The invention relates to the technical field of preparation and application of biocompatible carrier materials, in particular to the preparation and application of a modified hydroxyapatite microcapsule biomaterial carrier. Background technique [0002] In recent years, the field of biomaterials has developed rapidly, and the research and application of inorganic biomedical materials are very active. Among them, the research and clinical application of hydroxyapatite (HA or HAP) active ceramic materials have attracted much attention. application. Hydroxyapatite is a surface-active material, which has similar chemical composition and structure to biological hard tissues, so it has good biological activity and compatibility. Internationally, a variety of hydroxyapatite composite materials, coating materials and nanomaterials have been developed by using different processes, and breakthroughs have been made. The development of functional hydroxyapatite c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/24A61K47/04A61K9/52
Inventor 孙杰尹杉杉张玉峰张泽会李玫
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES