Method for preparing high-purity paeoniflorin

Abstract

The invention relates to a method for preparing high-purity paeoniflorin. The method comprises the steps of smashing the medicinal material-radix paeoniae alba; screening by a 50-mesh sieve; soaking with 70-80% ethanol solution which is 6-8 times as much as the smashed medicinal material-radix paeoniae alba for 1-2 hours; extracting by ultrasonic waves and microwaves, wherein the power of ultrasonic waves is controlled to be 600W(normally open), and the power of microwaves is controlled to be 500-800W; extracting for 1-4 hours; centrifuging; taking the supernate; concentrating the extracting solution to obtain extract; dissolving into water; extracting by using petroleum ether and ethyl acetate; taking the water layer; adding n-butyl alcohol and extracting 2-4 times; mixing the upper-phase organic solutions; decoloring by using activated carbon; concentrating at reduced pressure to obtain crude extract; and recrystallizing to obtain the high-purity paeoniflorin. The method reduces loss of effective components since the step of chromatographic column separation is omitted; and therefore the method has advantages of simple operation, short production cycle and high product purity, and is applicable to industrial production.

Description

technical field [0001] The invention relates to a method for preparing high-purity paeoniflorin, which belongs to the field of extraction and separation of active ingredients of natural plants. Background technique [0002] Radix Paeoniae Alba is the dry root of Paeoniae Alba, a plant of the Ranunculaceae family. It is slightly cold in nature, bitter and sour in taste, and has the functions of calming the liver and relieving pain, nourishing blood and regulating menstruation, astringing yin and stopping sweating, etc. It is mainly used for headache, dizziness, rib pain, limb pain, Blood deficiency and chlorosis, irregular menstruation, spontaneous sweating, night sweats, etc. Paeoniflorin is the main active ingredient of Radix Paeoniae Alba. Modern pharmacological studies have shown that paeoniflorin has anti-inflammatory, analgesic, anti-convulsant and immune-regulating effects. It is widely used in clinical practice and has definite curative effects. Flow, anti-acute myoc...

AI Technical Summary

Problems solved by technology

The overall defects of these extraction methods and purification means are that there are many impurities in paeoniflorin, the extraction rate is low, and the solvent consumption and residue are large.

For example, patent ZL2008101066912 discloses "a method for preparing total glucosides of paeony extract", which uses ethanol, methanol and other hydrophilic solvents for extraction, macroporous resin adsorption, eluting with alcohol solution, and concentrating the eluent to obtain peony Glycoside, the problem that this kind of method exists is: flow process is long, and solvent consumption is big, and a small part of paeoniflorin is lost when macroporous adsorption resin is purified, and the yield of product is reduced