Unlock instant, AI-driven research and patent intelligence for your innovation.

A method for extracting cordycepin from Cordyceps militaris

A technology for cordycepin and cordycepin militaris, applied in chemical instruments and methods, preparation of sugar derivatives, sugar derivatives, etc., can solve the problems of low purity of cordycepin, long operation time, low extraction efficiency, etc., and achieve strong operability. , Simple equipment, high separation efficiency

Inactive Publication Date: 2016-05-11
深圳市大成草生物科技有限公司
View PDF3 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims to solve the problems of high energy consumption, long operation time, low extraction efficiency and low purity of the obtained cordycepin in the prior art cordycepin extraction method, and provides a method for extracting cordycepin from Cordyceps militaris, which is effective for Cordyceps The extraction and purification process of cordycepin has been optimized. The process steps and selected equipment are simple, easy to operate, low energy consumption, and suitable for large-scale industrial production. The yield and purity of cordycepin are high, which provides a solid foundation for the industrial production of cordycepin technical basis

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) After crushing 300Kg of dried fruiting bodies of Cordyceps militaris to 30 meshes, add water and perform ultrasonic extraction twice under vacuum conditions. The amount of water added is 10 times the mass of the extracted raw materials. 2 times, each ultrasonic extraction time is 90min, and the extracts are combined.

[0028] (2) After coarse filtering the extract in step (1), pass it through 732-type cationic resin to obtain the first effluent.

[0029] (3) Settling the first effluent and collecting the supernatant, passing the supernatant through the 732-type cationic resin to obtain the second effluent.

[0030] (4) Pass the second effluent through a microporous membrane with a pore size of 3 μm for fine filtration to obtain a fine filtrate.

[0031] (5) Concentrate the fine filtrate by reverse osmosis to obtain the first concentrated liquid. The process conditions for reverse osmosis concentration are as follows: the temperature of the fine filtrate is 20°C, th...

Embodiment 2

[0037] (1) After crushing 300Kg of dry Cordyceps militaris solid medium to 10 mesh, add water and perform ultrasonic extraction twice under vacuum conditions. The amount of water added is 20 times the mass of the extracted raw material. The number of times is 2, the time of the first and second ultrasonic extraction is 90min and 80min respectively, and the extracts are combined.

[0038] (2) After coarse filtering the extract in step (1), pass it through 732-type cationic resin to obtain the first effluent.

[0039] (3) Settling the first effluent and collecting the supernatant, passing the supernatant through the 732-type cationic resin to obtain the second effluent.

[0040] (4) Pass the second effluent through a microporous membrane with a pore size of 4 μm for fine filtration to obtain a fine filtrate.

[0041] (5) Concentrate the fine filtrate by reverse osmosis to obtain the first concentrated liquid. The process conditions for reverse osmosis concentration are as follo...

Embodiment 3

[0047] (1) After crushing 300Kg of dried Cordyceps militaris mycelium to 20 mesh, add water and perform ultrasonic extraction three times under vacuum conditions. The amount of water added is 15 times the mass of the extracted raw material. 3 times, each ultrasonic extraction time is 60min, and the extracts are combined.

[0048] (2) After coarse filtering the extract in step (1), pass it through 732-type cationic resin to obtain the first effluent.

[0049] (3) Settling the first effluent and collecting the supernatant, passing the supernatant through the 732-type cationic resin to obtain the second effluent.

[0050] (4) Finely filter the second effluent through a microporous membrane with a pore size of 5 μm to obtain a fine filtrate.

[0051] (5) Concentrate the fine filtrate by reverse osmosis to obtain the first concentrated liquid. The process conditions for reverse osmosis concentration are as follows: the temperature of the fine filtrate is 30°C, the pressure of ente...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
pore sizeaaaaaaaaaa
Login to View More

Abstract

The invention discloses a method for extracting cordycepin from cordyceps militaris, and is used for solving the problem that the cordycepin extraction method in the prior art is high in energy consumption, long in operation time, low in extraction efficiency and low in purity of obtained cordycepin. The method comprises the main steps of vacuum ultrasound water extraction, twice 732 type cation anti-adsorption impurity removal, refined filtration, first reverse-osmosis concentration, 732 type cation purification, 717 type anion resin purification, second reverse-osmosis concentration and evaporative recrystallization. According to the method, the cordycepin extraction and purification processes are optimized, the process steps and the selected equipment are simple, the operability is strong, the energy consumption is low, large-scale industrial product can be realized, the yield and the purity of the cordycepin are high and technical base is provided to the industrial production of the cordycepin.

Description

technical field [0001] The invention relates to a method for extracting cordycepin, in particular to a method for extracting cordycepin from Cordyceps militaris. Background technique [0002] Cordyceps militaris, also known as Cordyceps militaris, belongs to the genus Cordyceps genus of the family Ascomycetes, and its medicinal active ingredient is mainly cordycepin. Due to the influence of raw materials, purification process and other factors, its price has remained high, and the high price has become a bottleneck restricting the development and utilization of cordycepin. Therefore, studying the extraction process of cordycepin and improving its yield has important economic value and broad market application potential. [0003] Publication No. CN101508715A, the Chinese patent published on August 19, 2009 discloses a method for extracting and purifying cordycepin from Cordyceps militaris, comprising: pulverizing the raw material of Cordyceps militaris and mixing it with wat...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07H19/16C07H1/08
Inventor 刘安球程倩刘高洁牛国成李文龙曲海明
Owner 深圳市大成草生物科技有限公司