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A human papillomavirus nucleic acid typing detection kit

A technology of human papillomavirus and detection kit, which is applied in the determination/inspection of microorganisms, microorganisms, biochemical equipment and methods, etc., and can solve the problems of high price, time-consuming, unsuitable for large-scale use, and restricted application

Active Publication Date: 2016-04-27
JIANGSU TONEKER MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Nucleic acid hybridization has good specificity, but the operation is complicated and time-consuming, which is not suitable for large-scale clinical use
The gene chip method can detect multiple subtypes in the same sample, but it is expensive, which greatly restricts its clinical application

Method used

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  • A human papillomavirus nucleic acid typing detection kit
  • A human papillomavirus nucleic acid typing detection kit
  • A human papillomavirus nucleic acid typing detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0107] Embodiment 1: the composition of kit

[0108] The composition of human papillomavirus (15 types) nucleic acid typing detection kit is as shown in table 1:

[0109] Table 1 The composition of human papillomavirus (15 types) nucleic acid typing detection kit

[0110]

[0111]

[0112] Wherein, the DNA extraction solution is TE buffer solution (pH9.0) containing 5% Chelex-100.

[0113] In the present invention, the DNA extraction solution can also be: comprising (1mM, pH5.0) or (2mM, pH6.0) or (5mM, pH7.0) or (8mM, pH8.0) or (10mM, pH9.0) 0) of EDTA, (1 mM, pH5.0) or (2 mM, pH6.0) or (5 mM, pH7.0) or (8 mM, pH8.0) or (10 mM, pH9.0) of Tris-HCl and volume Fractions (5%, 8%, 9%, 10%) of Chexel-100.

[0114] Among them, the final concentration of each component in HPV16, 58, 35, and HBB-PCR reaction solution is 1×PCR buffer, each primer is 100nM, each probe is 50nM, dNTPs (0.4mMdATP, 0.4mMdGTP, 0.4mMdCTP, 0.2mMdTTP, 0.2mMdUTP), 4mMMgCl 2 ;

[0115] In the present ...

Embodiment 2

[0125] Embodiment 2: the kit listed in above-mentioned embodiment 1 is used for the usage method of human papillomavirus (15 types) nucleic acid typing detection

[0126] (1) Collection and storage of samples

[0127] Sample type used: The detectable sample is cervical exfoliated cells.

[0128] Sampling method: Use a cotton swab soaked in normal saline to gently wipe off the excessive secretions from the cervix, use a cervical sampling brush to insert into the cervix and rotate it slightly for 4 to 5 weeks, take out the cervical sampling brush, and put it into the In the sample tube filled with cell preservation solution. Break off the excess brush handle at the mouth of the tube, leave the brush head in the sample tube, tighten the tube cap, and send the sample for inspection immediately.

[0129] Sample storage: The collected samples should be sent for inspection as soon as possible. It is recommended that the storage should not exceed 12 hours at 4°C, and should not exce...

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Abstract

The invention provides a kit for carrying out genotyping detection on human papilloma viral nucleic acid. The kit is characterized by comprising a DNA extracting solution, an HPV16, 58, 35, HBB-PCR reaction liquid, an HPV18, 33, 51, 66-PCR reaction liquid, an HPV45, 59, 39, 56-PCR reaction liquid, an HPV31, 52, 68, 53-PCR reaction liquid, a Taq / UNG enzyme mixed liquid, positive control and negative control. The kit provided by the invention can be used for detecting the human papilloma viral nucleic acid in a cervical secreta sample.

Description

technical field [0001] The invention belongs to the field of in vitro diagnostic reagents, in particular to a kit, more specifically to a human papillomavirus (15 types) nucleic acid typing detection kit, which is mainly suitable for detecting human papillomavirus in cervical secretion samples. Presence of papillomavirus (15 types) nucleic acid. Background technique [0002] Cervical cancer is a common malignant tumor of the female reproductive system, with about 500,000 new cases every year in the world, and a mortality rate of 50%. WHO pointed out that if measures are not taken as soon as possible, the number of deaths caused by cervical cancer will increase by about 25% in the next ten years. A large number of clinical experiments have shown that 99.7% of cervical cancers can detect human papillomavirus (humanpapillomavirus, HPV) DNA, and persistent infection of HPV is a necessary condition for the occurrence of cervical cancer. [0003] HPV is a group of double-strande...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/70C12Q1/68C12R1/93
CPCC12Q1/686C12Q1/70C12Q2531/113C12Q2545/101C12Q2563/107
Inventor 宋冬梅刘军辉刘代新
Owner JIANGSU TONEKER MEDICAL TECH
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