Method for detecting acetamiprid by using unlabeled type fluorescence sensor based on aptamer

A nucleic acid aptamer and fluorescent sensor technology, applied in the field of analytical chemistry, can solve the problems of high cost, long measurement time, unsuitable for rapid detection, etc., and achieve the effect of rapid detection

Inactive Publication Date: 2014-10-01
JILIN UNIV
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Problems solved by technology

Although these methods have high sensitivity, these methods require expensive equipment, long measurement time, high cost, professional and technical personnel are required to operate, and are not suitable for on-site rapid detection

Method used

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  • Method for detecting acetamiprid by using unlabeled type fluorescence sensor based on aptamer
  • Method for detecting acetamiprid by using unlabeled type fluorescence sensor based on aptamer
  • Method for detecting acetamiprid by using unlabeled type fluorescence sensor based on aptamer

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Embodiment Construction

[0036] Synthesis and Purification of CdTe Quantum Dots

[0037] Materials / Reagents: Te powder, NaBH4 and TGA were purchased from Sinopharm Reagent

[0038]Method: First weigh 0.0256g Te powder and 0.0386g NaBH4 into a three-necked flask, use high-purity water to prepare 100mL of NaOH solution with pH=11, pour N2 into it for 10min, put it in a constant pressure titration funnel ①, and use high-purity water to prepare Add 67 μL TGA to 100 mL of CdCl2 solution with a concentration of 4×10-3 mol / L, adjust the pH to 11 with 1 mol / L NaOH solution, inject N2 into it for 10 min, and put the obtained clear and transparent solution in another constant pressure titration In the funnel ②, connect the whole device with the vacuum and N2 system, and go through several steps of vacuuming and N2 to remove the O2 in the system; heat slightly, open the funnel ①, add 3 to 5 drops of water, and stir it electromagnetically to initiate the reaction. After the Te powder basically reacts completely ...

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Abstract

The invention discloses a method for detecting acetamiprid by using an unlabeled type fluorescence sensor based on an aptamer and belongs to the technical field of analytical chemistry. The method is characterized by detecting residual quantity of acetamiprid by using a fluorescence quenching effect of gold nanoparticles on quantum dots; the method comprises the following steps: synthesizing and purifying CdTe quantum dots; preparing the gold nanoparticles; detecting a circular dichroism spectrum for observing specific binding of the aptamer with the acetamiprid; detecting through a TEM (Transmission Electron Microscopy) and a DLS (Dynamic Light scatterometer) for observing a protection effect of the aptamer on the gold nanoparticles; and detecting the acetamiprid by using the fluorescence quenching effect of the gold nanoparticles on the CdTe quantum dots and detecting practical samples. The method is capable of simply, quickly and sensitively detecting the acetamiprid; moreover, the method has high sensitivity and provides convenience for research, production, supervision and the like in the future.

Description

technical field [0001] The invention discloses a method for detecting acetamiprid by a non-labeled fluorescent sensor based on a nucleic acid aptamer, belonging to the technical field of analytical chemistry. Background technique [0002] Acetamiprid has another name called Mobilang, Acetamiprid, NI-25, and its common name in English is acetamiprid. In 1996, it was developed by Japan Soda Co., Ltd. following imidacloprid (Bayer, Germany) in 1991 and nitenpyram (nitenpyram) in 1995. , Takeda, Japan) developed and commercialized the third chlorinated nicotinic insecticide. The basic characteristics are: high polarity, good water solubility, long stable half-life in aqueous solution, not easy to hydrolyze, strong systemic property, broad-spectrum insecticidal and long-lasting effect, and can be degraded by microorganisms. [0003] Domestic research on acetamiprid's aquatic ecological safety shows that it is highly toxic to some fish and low toxic to algae, so the pesticide sho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N33/566
Inventor 孙春燕郭佳佳许景月李莹罗叶丽沈斐
Owner JILIN UNIV
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