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Method for extracting selenocystine and selenomethionine from polyselenium plant Camelina officinalis

A technology of selenocystine and selenomethionine, which is applied in food science, metabolic diseases, drug combination and other directions, can solve problems such as waste of resources, and achieve the effects of high product extraction rate, wide application and simple technical process

Active Publication Date: 2016-03-23
恩施硒谷科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The Enshi State Selenium Research Institute found that the broken rice chestnut not only contains high selenocystine, but also has a certain amount of selenomethionine. It is a biological selenium resource with high utilization value. Although it can be eaten directly, Since it has not passed food certification, it cannot be added to food, resulting in a huge waste of resources

Method used

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  • Method for extracting selenocystine and selenomethionine from polyselenium plant Camelina officinalis
  • Method for extracting selenocystine and selenomethionine from polyselenium plant Camelina officinalis

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Embodiment 1

[0051] The present invention is a method for extracting selenocystine and selenomethionine from the broken rice chestnut of the selenium-accumulating plant. Water, ultrasonic for 60min, filtered, the filter residue was extracted twice with water, the residue was washed, the filtrate was combined, and the volume was adjusted to 200mL, and detected by HPLC-HG-AGS, the chromatographic conditions were: anion exchange column, A mobile phase 40mM, B mobile phase 60mM (Adjust pH value to 6.0 with formic acid), flow rate 1.0mL / min, adopt 0min~3min: 100%A; 3min~4min: 100%A→100%B; 4min~10min: 100%B; 10min~12.5min: Gradient elution of 100% A, selenocystine retention time is 2.42±0.25min, selenocystine content in the extract is 952.25mg / kg; use high-pressure preparation system to separate, the conditions are the same as the above chromatography, collect 2. For the solution between 10 and 2.70 minutes, put the eluate into a cellulose ester dialysis bag with a molecular weight cut-off of 10...

Embodiment 2

[0053] The present invention is a method for extracting selenocystine and selenomethionine from the selenium-accumulating plant Broken Camelina. The LHCl solution was shaken at 60°C for 90 minutes, the filter residue was extracted twice with water, the residue was washed, the filtrate was combined, the pH value was adjusted to 6-7 with NaOH solution, and the volume was adjusted to 200mL. The selenium in the extract was detected by HPLC-HG-AGS. The content of cysteine ​​is 747.00mg / kg, and the content of selenomethionine is 216.34mg / kg. It is separated by a high-pressure preparation system, and the conditions are the same as the above chromatography. The selenocystine solution and 3. The selenomethionine solution between 10 and 3.70 minutes was weighed after dialysis and freeze-drying, respectively 5.35mg and 1.88mg, and the purity was 98.4% and 98.8%, respectively.

Embodiment 3

[0055] The present invention is a method for extracting selenocystine and selenomethionine from the selenium-accumulating plant cinnamon, the specific steps are as follows: Weigh 10 g of the selva sample with a selenium content of 2158.97 mg / kg, add 50mL1mol / L HCl, Ultrasonic extraction for 60 min, the filter residue was extracted twice again, the residue was washed, the filtrate was combined, the pH value was adjusted to 6-7 with NaOH solution, and the volume was adjusted to 200mL. The content of selenocystine in the extract was detected by HPLC-HG-AGS. 763.56mg / kg, the content of selenomethionine is 223.16mg / kg, separated by high-pressure preparation system, the conditions are the same as the above chromatographic conditions, selenocystine solution and selenomethionine solution are collected in sections, dialyzed, freeze-dried, weighed, They were 6.18mg and 1.95mg respectively, and the purity was 98.5% and 98.6%.

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Abstract

The invention relates to a method for extracting selenocystine and selenomethionine from selenium-gathered plant Cardamine hirsuta. The method comprises the following steps: after crushing a dried poly-selenium plant Cardamine hirsute material, extracting selenocystine by adding hydrochloric acid, extracting selenomethionine by adding sodium hydroxide, regulating the pH value of a filtrate to be nearly neutral, separating by using a high pressure preparation column, removing inorganic salt ions of an eluent containing selenocystine and selenomethionine by respectively using cellulose ester dialysis bags, and then, carrying out freeze drying to obtain high-quality selenocystine and selenomethionine products. The technical process is simple; the product extraction rate is high; the produced selenocystine and selenomethionine products can act as crude drugs for producing injections or food additives, are wide in use, and meanwhile can promote the development of Cardamine hirsuta planting industry, so that the industrial chain is extended.

Description

technical field [0001] The invention relates to a method for extracting selenocystine and selenomethionine from a selenium-accumulating plant orina. Background technique [0002] Selenium is one of the essential trace elements for the human body, selenocystine is the twenty-first amino acid necessary for the human body, and selenomethionine replaces methionine to synthesize proteins in the human body. More than 40 human diseases are related to selenium deficiency, but due to selenium It belongs to scattered elements, and its content on the earth is very small, resulting in selenium deficiency in about 72% of the world. In China, more than 900 million people need to supplement selenium. The annual demand for selenium is about 1,000 tons, of which 80% rely on imports. In the past, the most important method for people and animals to supplement selenium was to add sodium selenite to food and feed, or to eat selenium-enriched yeast tablets for patients, but the harm of sodium selen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C391/00A23L33/175A61K31/198A61P3/02
Inventor 陈永波胡百顺李红英黄光昱石月明
Owner 恩施硒谷科技股份有限公司