Novel enhanced Raman micro-needle with inbuilt enhanced layers and preparation method for novel enhanced Raman micro-needle

A Raman microneedle and reinforcement layer technology, which is applied in the field of enhanced Raman material preparation, can solve the problems of reducing Raman signal intensity, affecting the diffusion of tissue fluid, and weakening the enhancement effect of microneedles, so as to avoid attenuation and improve analysis sensitivity.

Inactive Publication Date: 2014-10-15
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] However, this method also has disadvantages. The nanoparticles that enhance the Raman effect on the surface of the needle body are adsorbed. Therefore, these nanoparticles are easily rubbed off during the process of entering and leaving the human body, resulting in weakened enhancement effect of the microneedle. ; coated with a microporous polymer layer is able to protect these nanoparticles from erasing
But it will affect the diffusion of interstitial fluid and reduce the intensity of Raman signal
In addition, the heat source substances contained in the polymer will also cause adverse effects on the human body.

Method used

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  • Novel enhanced Raman micro-needle with inbuilt enhanced layers and preparation method for novel enhanced Raman micro-needle
  • Novel enhanced Raman micro-needle with inbuilt enhanced layers and preparation method for novel enhanced Raman micro-needle
  • Novel enhanced Raman micro-needle with inbuilt enhanced layers and preparation method for novel enhanced Raman micro-needle

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Embodiment 1

[0026] A new type of enhanced Raman microneedle with a built-in reinforcement layer. It includes a needle handle 13, a needle body 12 and a needle tip 11 connected in sequence from top to bottom. There is a group of micropores on the surface of the needle body 12 and needle tip 11. A metal nanomaterial layer is covered, and the metal nanomaterial layer is a composite nanolayer composed of a gold nanometer layer and a silver nanometer layer, and the thickness of the metal nanomaterial layer is 20 nanometers. Micropore diameter 20 microns, depth 5 microns.

[0027] The preparation method of the above-mentioned novel enhanced Raman microneedle built in the reinforcement layer comprises the following steps:

[0028] 1) Insert the needle body and needle tip of the microneedle into the polymer solution and let it stand for 30 seconds to obtain a polymer layer. The polymer mass and solvent volume ratio in the polymer solution is 0.1%, and the polymer layer is is polystyrene;

[002...

Embodiment 2

[0033] A new type of enhanced Raman microneedle with a built-in reinforcement layer. It includes a needle handle 13, a needle body 12 and a needle tip 11 connected in sequence from top to bottom. There is a group of micropores on the surface of the needle body 12 and needle tip 11. A metal nanomaterial layer is covered, and the metal nanomaterial layer is one or more of gold nanolayer, silver nanolayer, copper nanolayer or platinum nanolayer, and the thickness of the metal nanomaterial layer is 2500 nanometers. The micropores are 5 microns in diameter and 25 microns in depth.

[0034] The preparation method of the above-mentioned novel enhanced Raman microneedle built in the reinforcement layer comprises the following steps:

[0035] 1) Insert the needle body and needle tip of the microneedle into the polymer solution and let it stand for 1 second to obtain a polymer layer. The polymer mass and solvent volume ratio in the polymer solution is 10%, and the polymer layer is It i...

Embodiment 3

[0040] A new type of enhanced Raman microneedle with a built-in reinforcement layer. It includes a needle handle 13, a needle body 12 and a needle tip 11 connected in sequence from top to bottom. There is a group of micropores on the surface of the needle body 12 and needle tip 11. A metal nanomaterial layer is covered, and the metal nanomaterial layer is one or more of gold nanolayer, silver nanolayer, copper nanolayer or platinum nanolayer, and the thickness of the metal nanomaterial layer is 1260 nanometers. The diameter of the micropore is 50 microns, and the depth is 50 microns.

[0041] The preparation method of the above-mentioned novel enhanced Raman microneedle built in the reinforcement layer comprises the following steps:

[0042] 1) Insert the needle body and needle tip of the microneedle into the polymer solution and let it stand for 60 seconds to obtain a polymer layer. The polymer mass and solvent volume ratio in the polymer solution is 5%, and the polymer layer...

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Abstract

The invention discloses a novel enhanced Raman micro-needle with inbuilt enhanced layers. The novel enhanced Raman micro-needle comprises a needle handle, a needle body and a needle tip, which are connected sequentially from top to bottom, wherein a group of micro-holes are formed in the surfaces of the needle body and the needle tip; a metallic nano material layer covers the inner wall of each micro-hole. Compared with a conventional SERS (Surface Enhanced Raman Scattering) active micro-needle, the novel enhanced Raman micro-needle has the advantages that enhanced Raman layers are inbuilt, and macromolecule coating layers are not needed, so that the attenuation of a signal can be avoided, and the adverse effects on an organism caused by heat source substances contained in macromolecule layers are further avoided. Compared with existing surface enhanced Raman materials, the enhanced Raman layers of the novel enhanced Raman micro-needle are only gathered in the micro-holes, the dimensions of the micro-holes and the sizes of detected laser light spots are in the same order of magnitude, and most of adsorbed molecules are effective detection molecules, so that the analysis sensitivity is further improved favorably.

Description

technical field [0001] The present invention relates to the technical field of preparation of Raman-enhanced materials, more specifically, to a novel microneedle with enhanced Raman activity built in a reinforcement layer and a preparation method thereof. Background technique [0002] Using acupuncture needles as a carrier to absorb nanoparticles with enhanced Raman effects, a Raman-active microneedle that integrates sampling and detection functions into and out of the human body is constructed. When the microneedle enters the body or tissue, the interstitial fluid will diffuse into the gap on the surface of the microneedle, and when the microneedle is pulled out, the molecules to be tested in the interstitial fluid can be carried out. In vitro surface-enhanced Raman spectroscopy detection can avoid the attenuation of Raman signals in tissues, and at the same time, the enhanced Raman signals of a certain depth of subcutaneous tissue can be obtained according to the detection...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61B5/00A61B10/00A61H39/08A61K49/00
Inventor 董健辛丽妃谢凡朱珠张里钱卫平
Owner SOUTHEAST UNIV
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