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Preparation method for rebaudiodside A molecularly-imprinted polymer

A technology of molecular imprinting and polymers, which is applied in the fields of alkali metal compounds, chemical instruments and methods, alkali metal oxides/hydroxides, etc., can solve problems such as the preparation method of molecularly imprinted polymers of rebaudioside A that have not been found yet, Achieve good recognition ability and adsorption selectivity, good binding ability and separation performance, and simple process

Active Publication Date: 2014-10-15
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] By consulting the prior art literature, no report related to the preparation method of the molecularly imprinted polymer of rebaudioside A in the content of the present invention has been found

Method used

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  • Preparation method for rebaudiodside A molecularly-imprinted polymer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1: The template molecule Rebaudioside A, the functional monomer diethylaminoethyl methacrylate, and the crosslinking agent ethylene glycol dimethacrylate were dissolved in the porogen (N,N'-dimethyl Formamide: tetrahydrofuran = 2:1, volume ratio), placed in an ampere flask, repeatedly oscillated on an oscillator at room temperature to make the template molecules and functional monomers fully interact, and then add the initiator azobisisobutyronitrile, After ultrasonic treatment (ultrasonic power 250W, treatment time 1h), fill with nitrogen gas (time 15min), and vacuum seal the tube; the ratio of the amount of template molecule to functional monomer is 1:8, functional monomer and crosslinking agent The ratio of the amount of the substance is 1:5, the volume ratio of the crosslinking agent to the porogen is 1:1, and the mass ratio of the initiator, the functional monomer and the crosslinking agent is 1:100; the mixed solution is in Thermally initiate polymerization...

Embodiment 2

[0038] Example 2: The template molecule Rebaudioside A, the functional monomer diethylaminoethyl methacrylate, and the crosslinking agent ethylene glycol dimethacrylate are dissolved in the porogen (N,N'-dimethyl Formamide: tetrahydrofuran = 1:1, volume ratio), placed in an ampoule, repeatedly oscillated on an oscillator at room temperature to make the template molecules and functional monomers fully interact, and then add the initiator azobisisobutyronitrile, After ultrasonic treatment (ultrasonic power 300W, treatment time 1h), fill with nitrogen gas (time 25min), and vacuum seal the tube; the ratio of the amount of template molecule to functional monomer is 1:16, functional monomer and crosslinking agent The ratio of the amount of the substance is 1:4, the volume ratio of the crosslinking agent to the porogen is 1:3, and the mass ratio of the sum of the amount of initiator, functional monomer and crosslinking agent is 1:100; the mixed solution is in The polymerization reacti...

Embodiment 3

[0040] Example 3: The template molecule Rebaudioside A, the functional monomer diethylaminoethyl methacrylate, and the crosslinking agent ethylene glycol dimethacrylate are dissolved in the porogen (N,N'-dimethyl Formamide: tetrahydrofuran = 1:3, volume ratio), placed in an ampere flask, repeatedly oscillated on an oscillator at room temperature to make the template molecules and functional monomers fully interact, and then add the initiator azobisisobutyronitrile, After ultrasonic treatment (ultrasonic power 400W, treatment time 1h), fill with nitrogen gas (time 30min), and vacuum seal the tube; the ratio of the amount of template molecule to functional monomer is 1:32, functional monomer and crosslinking agent The ratio of the amount of the substance is 1:3, the volume ratio of the crosslinking agent to the porogen is 1:5, and the mass ratio of the initiator, the functional monomer and the crosslinking agent is 1:100; the mixed solution is in Thermally initiate polymerization...

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Abstract

The invention relates to the field of food separation technology, specifically to a preparation method for a rebaudiodside A molecularly-imprinted polymer. According to the invention, rebaudiodside A is used as a template molecule, diethylaminoethyl methacrylate is used as a functional monomer, ethylene glycol dimethacrylate is used as a cross-linking agent, a mixed solvent of N,N'-dimethyl formamide and tetrahydrofuran is used as a pore forming agent, azodiisobutyronitrile is used as an initiator, and the rebaudiodside A molecularly-imprinted polymer is prepared through dispersion and uniform mixing of the above-mentioned substances, introduction of nitrogen for removal of oxygen, thermal-initiation polymerization, Soxhlet extraction and drying. The invention has the following beneficial effects: the prepared molecularly-imprinted polymer has good adsorption selectivity and great adsorption capacity on rebaudiodside A in a water-containing mobile phase and can be used as an adsorption and separation material for separating and purifying rebaudiodside A in stevia rebaudiana extract.

Description

Technical field [0001] The invention belongs to the technical field of food separation, and particularly relates to a method for preparing a rebaudioside A molecularly imprinted polymer. Background technique [0002] Sweeteners are a kind of food additives that play an important role in food processing characteristics and taste. Rebaudioside A is a low-calorie high-power sweetener extracted from the stems and leaves of the Compositae plant Stevia. It has excellent characteristics such as green, natural, safe and non-toxic, stable physical and chemical properties, and taste close to sucrose. Rebaudioside A as a plant extract, the current industrial-scale production of high-purity rebaudioside A is complicated. After the dried stevia leaves are crushed, extracted, impurity-removed, decolorized and purified, the crude product is obtained. It is also necessary to use alcohol solvents or alcohol-water mixed solvents through repeated recrystallization processes to obtain high-purity r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08F222/14C08F220/34C08J9/28C08J9/26B01J20/26B01J20/28B01J20/30
Inventor 杨瑞金叶发银华霄唐乐乐赵伟张文斌
Owner JIANGNAN UNIV
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