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Preparation method of 4-hydroxybutyric acid test paper resisting interference of ascorbic acid and pigments

A technology for detecting hydroxybutyric acid and test paper, which is applied in the direction of material analysis by observing the influence of chemical indicators, and analysis by making materials undergo chemical reactions, etc., which can solve the instability of normal temperature state, wrong results, interference detection results, etc. problem, to achieve good specificity and sensitivity, eliminate interference, and reliable results

Inactive Publication Date: 2014-10-22
武汉高丰生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

"Reliable, Sensitive, Rapid and Quantitative Enzyme-Based Assay for Gamma-Hydroxybutyric Acid (GHB)" (Dawn T.Bravo et al.) introduces the preparation of GHB dehydrogenase and the preparation of test paper for detecting ghb, but this method There are some serious problems: the test strips can only be stored in cold storage, and the normal temperature state is unstable; GHB dehydrogenase and ascorbic acid have cross-reactions, causing false positive reactions in samples with ascorbic acid, resulting in wrong results; many beverages themselves have color, which will interfere Test results

Method used

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  • Preparation method of 4-hydroxybutyric acid test paper resisting interference of ascorbic acid and pigments
  • Preparation method of 4-hydroxybutyric acid test paper resisting interference of ascorbic acid and pigments
  • Preparation method of 4-hydroxybutyric acid test paper resisting interference of ascorbic acid and pigments

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Embodiment 1

[0034] The preparation process that the total volume of the first phase enzyme solution liquid is 100mL is as follows:

[0035] 1) 50mmol / L Tris-hydrochloric acid buffer with pH 8.0;

[0036] 2) Dissolve 500mg of bovine serum albumin;

[0037] 3) Dissolved into NAD + 0.5g;

[0038] 4) Dissolve diaphorase, 10000iu;

[0039] 5) Dissolve in, 4-hydroxybutyrate dehydrogenase 100000iu.

[0040] The first phase enzyme reagent layer solution impregnation:

[0041] After the solution is prepared, add it to the dipping machine, install a roll of whatman23sl filter paper, set the drying temperature to 50°C, and set the paper speed to 3.0. Start to impregnate and move the paper. After the solution is soaked, the filter paper stays in the drying box for 1 hour to dry completely.

[0042] Preparation of the second phase developer layer solution:

[0043] 100ml of ethanol solution, dissolved in 400mg of nitro blue tetrazolium chloride.

[0044] Second phase solution impregnation:

...

Embodiment 2

[0059] The preparation process that the total volume of the first phase enzyme solution liquid is 100mL is as follows:

[0060] 1) 50mmol / L Tris-hydrochloric acid buffer with pH 8.0;

[0061] 2) Dissolve 500mg of bovine serum albumin;

[0062] 3) Dissolve NAD+0.5g;

[0063] 4) Dissolve diaphorase, 10000iu;

[0064] 5) Dissolve, 4-hydroxybutyrate dehydrogenase 50000iu.

[0065] The first phase enzyme reagent layer solution impregnation:

[0066] After the solution is prepared, add it to the dipping machine, install a roll of whatman23sl filter paper, set the drying temperature to 50°C, and set the paper speed to 3.0. Start to impregnate and move the paper. After the solution is soaked, the filter paper stays in the drying box for 1 hour to dry completely.

[0067] Preparation of the second phase developer layer solution:

[0068] 100ml of ethanol solution, dissolved in 300mg of nitro blue tetrazolium chloride

[0069] Second phase solution impregnation:

[0070] The 593...

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Abstract

The invention relates to a preparation method of a 4-hydroxybutyric acid test paper resisting the interference of ascorbic acid and pigments. The method includes: infiltrating a filter paper in a first phase immersion liquid, then conducting hot air drying at 30-50DEG C to obtain an enzyme reagent layer; infiltrating a filter paper in a second phase immersion liquid, then conducting hot air drying at 30-50 DEG C to obtain a color developing agent layer; infiltrating a sponge stick in an anti-ascorbic acid infiltration liquid, performing hot air drying at 40-60DEG C to obtain an anti-ascorbic acid sponge stick; and pressing test paper pieces obtained by cutting the color developing agent layer and the enzyme reagent layer under an upper cover color developing hole and at one end of the anti-ascorbic acid sponge stick in order, thus obtaining the test paper. The method provided by the invention provides a solution to the problems that: existing test papers can only be preserved under refrigeration and is unstable under normal temperature; GHB (gamma-hydroxybutyrate) dehydrogenase and ascorbic acid can undergo cross reaction to make ascorbic acid existing samples undergo false positive reaction, thereby obtaining wrong results; and many beverages have colors in itself, thus interfering detection results and like.

Description

technical field [0001] The invention belongs to the field of preparation of test paper for detecting 4-hydroxybutyric acid, in particular to a method for preparing a test paper for detecting 4-hydroxybutyric acid interfered with by ascorbic acid and pigment. Background technique [0002] GHB full name Gamma-Hydroxybutyrate (gamma-hydroxybutyric acid and its sodium salt) is a narcotic drug. In the early 1960s, GHB was used as an anesthetic in medicine. Later, when the adverse side effects of GHB were discovered, the use of GHB was stopped, and it was only used as a sleeping pill. Criminals began to use GHB for entertainment and crime in the 1990s. GHB is white powder, colorless when dissolved in water, and liquid is called G water. GHB is odorless and has a slightly salty taste. The reaction of GHB is similar to that of alcohol. In small doses, it can induce relaxation, release of personal defenses, high spirits and a high feeling similar to light drunk. Moderate amounts...

Claims

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Application Information

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IPC IPC(8): G01N21/78
Inventor 王福进管利丰张懿
Owner 武汉高丰生物科技有限公司
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