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A kind of extraction method of phycoerythrin

A technology of phycoerythrin and extraction method, which is applied in the field of biomedicine, can solve the problems of long cycle, protein denaturation and inactivation, low product yield, etc., and achieve the effect of improving production efficiency, short cycle and high purity

Active Publication Date: 2017-07-28
TAIZHOU BINMEI BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the use of the freeze-thaw method has the problem of a long period, and the use of the ultrasonic method can easily lead to protein denaturation and inactivation, and the product yield is low, and the use of the salting-out method also pollutes the environment relatively large

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Take fresh Porphyra zebra, weigh it, and then add ice powder at -50°C according to the weight ratio of Porphyra zebra and ice powder at a ratio of 1:20. The average particle size of the ice powder is 40-20 meshes. Put Porphyra zebra and ice powder into the stirring tank together, the Porphyra zebra is preferably pre-soaked, then control the stirring speed at 3000r / min and control the temperature of the system below 0°C to keep warm for wall breaking treatment 90 minutes, after the wall-breaking treatment, the ice powder in the system was naturally melted to obtain a wall-breaking solution containing phycoerythrin. After testing, the wall-breaking rate of algae cells reached more than 95%;

[0031] Insert the cotton column made of medical absorbent cotton into the above-mentioned wall-breaking solution containing phycoerythrin to make the phycoerythrin adsorb on the cotton column. The medical absorbent cotton can be fixed with a stainless steel filter, and the height of t...

Embodiment 2

[0035] Take fresh almond laver, weigh it, and then add ice powder at -40°C according to the weight ratio of altar laver and ice powder at a ratio of 1:15. The average particle size of the ice powder is 40-20 mesh. Put it into the stirring tank together with ice powder, the altar laver is preferably pre-soaked, then, control the stirring speed at 2000r / min and control the system temperature below -10°C to keep warm for 80min to break the wall. After the wall treatment is completed, the ice powder in the system is naturally melted to obtain a wall-breaking liquid containing phycoerythrin. After testing, the wall-breaking rate of algae cells reaches more than 95%;

[0036] Insert the cotton column made of medical absorbent cotton into the above-mentioned wall-breaking solution containing phycoerythrin to make the phycoerythrin adsorb on the cotton column. The medical absorbent cotton can be fixed with a stainless steel filter, and the height of the cotton column can be adjusted ac...

Embodiment 3

[0040] Take fresh algae laver, weigh it, and then add ice powder at -40°C according to the weight ratio of algae laver and ice powder at a ratio of 1:15. The average particle size of the ice powder is 40-20 mesh. It is made of 0.5-1.0mol / L sodium chloride aqueous solution, and the above-mentioned altar laver and ice powder are added into the stirring tank together. The altar laver is preferably soaked in advance, and then the stirring speed is controlled at 2000r / Under the condition of 1 min and control the temperature of the system between 0°C and -20°C, the wall-breaking treatment was carried out for 70 minutes. After the wall-breaking treatment, the ice powder in the system was naturally melted to obtain the wall-breaking liquid containing phycoerythrin. Tested, algae cell wall breaking rate reached over 95%;

[0041] Insert the cotton column made of medical absorbent cotton into the above-mentioned wall-breaking solution containing phycoerythrin to make the phycoerythrin ...

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Abstract

The invention relates to an extraction method of phycoerythrin, which belongs to the technical field of biological medicines. For solving existing problems of low wall-breaking efficiency and large pollution to the environment, the invention provides an extraction method of phycoerythrin and the method comprises the following steps: adding fresh algae containing phycoerythrin or pre-soaked algae powder into ice powder at a temperature of minus 50-minus 10 DEG C, and carrying out wall-breaking treatment on the obtained substance under the condition that the stirring speed is controlled at 1000-3000 r / min, so that a wall-breaking fluid containing phycoerythrin is obtained; and separating, purifying and drying the wall-breaking fluid containing phycoerythrin, so that a finished product phycoerythrin is obtained. The method has the advantages of short wall-breaking cycle, high efficiency and environmental friendliness, and obtained products are high in purity.

Description

technical field [0001] The invention relates to a method for extracting phycoerythrin, belonging to the technical field of biomedicine. Background technique [0002] Phycoerythrin is an important light-harvesting pigment protein of many seaweeds. It forms rod-shaped phycobilisomes with phycobiliproteins such as phycocyanin and allophycocyanin. Phycobilisomes can capture and transmit light energy. Phycoerythrin is mainly distributed in the red algae of seaweeds. Due to the existence of pigment molecules, it has strong absorption in the 480-570nm band of visible light and can produce strong fluorescence. Phycoerythrin can be used as a natural pigment in food, cosmetics, dyes and other industries, and can also be made into fluorescent reagents for clinical medical diagnosis and research fields such as immunochemistry and bioengineering. Phycoerythrin is also an important physiologically active substance, which can be made into a photosensitizer for photodynamic therapy of tumo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/405C07K1/34
CPCC07K14/795
Inventor 张兵权
Owner TAIZHOU BINMEI BIOTECH
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