Method for producing ademetionine by fermentation of saccharomycetes
A technology of adenosylmethionine and yeast, applied in the field of microorganisms, can solve the problems of late SAM preparation technology and low SAM fermentation level, and achieve the effects of low production cost, no pollution in the process, and simple production process
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0052] (1) Fermentation: Saccharomyces cerevisiae XJK12 obtained through mutagenesis screening was used as the starting strain, inserted into a 250mL Erlenmeyer flask containing 100mL seed medium under aseptic conditions, cultured at 180r / min, 28°C for 24h, and obtained seed liquid; The seed liquid is inserted into a 10L fermenter with 6L fermentation medium at 5% inoculation amount, at 28°C, with an air flow of 1-2L / min, a rotation speed of 300-500r / min, and keeping dissolved oxygen above 30%. After 10 hours, sucrose was added, and the concentration of sucrose was controlled at 40-45g / L; after 24 hours of fermentation, the precursor L-methionine was continuously fed at a rate of 1.5g / h until the fermentation was completed. The fermentation broth of glycosylmethionine, wherein the fermentation level of adenosylmethionine reaches 16.25g / L, and the dry weight of biomass reaches 171.46g / L;
[0053] The seed culture medium is composed of the following components in parts by weight...
Embodiment 2
[0061] (1) Fermentation: Saccharomyces cerevisiae XJK12 obtained through mutagenesis screening was used as the starting strain, inserted into a 250mL Erlenmeyer flask containing 100mL seed medium under aseptic conditions, cultured at 180r / min, 28°C for 24h, and obtained seed liquid; The seed liquid is inserted into a 10L fermenter with 6L of fermentation medium at 8% inoculation amount, at 28°C, with an aeration rate of 1-2L / min, a rotation speed of 300-500r / min, and keeping dissolved oxygen above 30%. After 10 hours, sucrose was added, and the concentration of sucrose was controlled at 40-45g / L; after 24 hours of fermentation, the precursor L-methionine was continuously fed at a rate of 1.5g / h until the fermentation was completed. The fermentation broth of glycosylmethionine, wherein the fermentation level of adenosylmethionine reaches 16.82g / L, and the dry weight of biomass reaches 171.29g / L;
[0062] The seed culture medium is composed of the following components in parts b...
Embodiment 3
[0070] (1) Fermentation: Saccharomyces cerevisiae XJK12 obtained through mutagenesis screening was used as the starting strain, inserted into a 250mL Erlenmeyer flask containing 100mL seed medium under aseptic conditions, cultured at 180r / min, 28°C for 24h, and obtained seed liquid; Put the seed liquid into a 10L fermenter with 6L fermentation medium at 10% of the inoculation amount, at 28°C, with an aeration rate of 1-2L / min, a rotation speed of 300-500r / min, and keep dissolved oxygen above 30%. After 10 hours, sucrose was added, and the concentration of sucrose was controlled at 40-45g / L; after 24 hours of fermentation, the precursor L-methionine was continuously fed at a rate of 1.5g / h until the fermentation was completed. The fermentation broth of glycosylmethionine, wherein the fermentation level of adenosylmethionine reaches 16.38g / L, and the dry weight of biomass reaches 171.59g / L;
[0071] The seed culture medium is composed of the following components in parts by weig...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More