Anti-type Ⅱ diabetes long-acting nanocomposite peptide and its preparation method and application

Abstract

The invention discloses a long-acting nanometer composite peptide resistant to II-type diabetes and a preparing method and application of the long-acting nanometer composite peptide. Nano-selenium serves as a carrier, chitosan performs a connection function, and accordingly covalent binding of active polypeptides and nano-selenium particles is achieved. The prepared nanometer composite peptide has the functions of carrying, protecting and slowly releasing therapeutic polypeptides and effectively prolonging the half-life period of the therapeutic polypeptides in the physiological environment. Effective dosage of the peptide with a therapeutic effect can be obviously lower than dosage of the therapeutic polypeptides which are used separately, and the biological functions of medicine slow releasing and long-acting treatment can be performed. The defect that when used separately, the therapeutic polypeptides are prone to being quickly metabolized and removed in the body due to the fact that the molecular weight of the therapeutic polypeptides is small is overcome, and the half-life period and bioavailability of the small-molecule therapeutic polypeptides in the body can be effectively improved. The nanometer composite peptide can be used for preparing medicines for treating the following diseases of diabetes and hyperglycemia. The preparing method is high in efficiency and low in cost and the application prospect is wide.

Description

technical field [0001] The invention belongs to the field of nano-biotechnology, and in particular relates to a long-acting nano-composite peptide for anti-type 2 diabetes and its preparation method and application. Background technique [0002] Pituitary adenylate cyclase-activating peptide (PACAP) is a neuropeptide with important biological functions discovered in 1990 and secreted by the pituitary gland. It is a new member of the secretin / glucagon / VIP family. PACAP exists in two forms: PACAP38, which consists of 38 amino acids; PACAP27, which consists of 27 amino acids at the N-terminal of PACAP38. PACAP exerts extensive and important biological functions by activating its specific G protein-coupled receptors and increasing the concentration of second messengers such as cAMP in target cells. PACAP has three types of G protein-coupled receptors: PAC1, VPAC1 and VPAC2; the three types of receptors have different distributions in organisms and play different roles. Among t...

AI Technical Summary

Problems solved by technology

[0006] VPAC2 receptor-specific agonist active peptides have good glucose-dependent hypoglycemic effect, but often have a small molecular weight, short half-life in vivo, and reduced bioavailability. The biological role of polypeptides, coupling these active polypeptides with chitosan-modified nanocarrier particles as a new drug system can effectively improve the water solubility and in vivo stability of polypeptide drugs, control the release of drugs in vivo and target selectivity, Effectively prolong the half-life of active peptide drugs, which has important medicinal value

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