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Method for improving microalgae growth rate and oil yield

A growth rate and microalgae technology, which is applied in the field of improving the growth rate and oil yield of microalgae, can solve the problem that single-gene knockout mutants cannot grow normally, and achieve the effects of improving growth rate, high efficiency and broad application prospects.

Inactive Publication Date: 2014-11-12
SHANGHAI ADVANCED RES INST CHINESE ACADEMY OF SCI
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Problems solved by technology

The single-gene knockout mutant just constructed couldn't even grow normally

Method used

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  • Method for improving microalgae growth rate and oil yield
  • Method for improving microalgae growth rate and oil yield
  • Method for improving microalgae growth rate and oil yield

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Embodiment 1

[0027] Microalgal strains used: Chlamydomonas reinhardtii wild strain CC4324 (Ball 330 arginine auxotrophy, no cell wall), mutant strain CC4326 (AGPase large subunit inactivation) and mutant strain CC4334 (isoamylase inactivation ) as an example to illustrate the feasibility of this technical solution. The above algae species are all from the Chlamydomonas Resource Center of the United States.

[0028] The steps for adaptive evolution of microalgal strains are as follows:

[0029] 1) The basal medium of the three microalgae strains is TAP liquid medium, at 25°C and 50μmol photons m -2 the s -1 cultured in a shaking incubator with continuous light. The first generation of microalgae strains (CC4324, CC4326 and CC4334) were measured by the optical density OD at 680nm 680 Under the condition of 0.1, each was inoculated in 50mL TAP medium (100mL Erlenmeyer shake flask), cultivated for 3 days, and measured the dry weight.

[0030] 2) Take the first-generation microalgae strain...

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Abstract

The invention discloses a method for improving microalgae growth rate and oil yield, and the method includes the following steps: 1), after inoculation of microalgae, the microalgae is cultured for 2-5 days by continuous illumination or cycled illumination in a certain light and dark conditions at 20-35 DEG C and in the conditions of 20-200 mu mol photon in m<-2>s<-1>; 2), the microalgae strain obtained by the step 1) is inoculated again, and then cultured; and 3), the microalgae strain is cultured by step 1) as a training cycle, and then cultured by step 2) as a next training cycle, and the microalgae strain with the growth rate and oil yield both increased can be obtained by the cycled training. According to the method, the growth rate and oil yield both of wild microalgae strains and microalgae gene deficient mutants are increased, and compared with the large-scale screening, the method is less in workload and high in efficiency, is a new strain selection method, and has broad application prospects in microalgae biofuel development.

Description

technical field [0001] The invention relates to a method for improving the cultivation of microalgae, in particular to a method for increasing the growth rate and oil yield of microalgae. Background technique [0002] With the rapid development of my country's economy, the energy demand gap is increasing year by year. According to the 2012 Statistical Bulletin on National Economic and Social Development, my country imported 271 million tons of crude oil and 288 million tons of coal last year. Fossil energy also has the problem of greenhouse gas emissions in the process of mining and use, leading to climate anomalies and natural disasters. The "Kyoto Protocol" implemented in 2005 obliges countries to undertake greenhouse gases, especially CO 2 The obligation to reduce emissions has been clearly stipulated, and carbon reduction is imperative. The development of renewable energy can reduce dependence on fossil energy and effectively control CO 2 Emissions have become a majo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
Inventor 赵权宇于水燕史吉平
Owner SHANGHAI ADVANCED RES INST CHINESE ACADEMY OF SCI
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