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A cultivation method for promoting growth of transgenic tomato and accumulation of astaxanthin

A cultivation method, transgenic technology, applied in the fields of botanical equipment and methods, horticulture, application, etc.

Inactive Publication Date: 2016-02-24
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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  • Claims
  • Application Information

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Problems solved by technology

At present, how to promote the growth of astaxanthin transgenic tomato plants and the accumulation of astaxanthin content in fruits by adjusting the environmental conditions of facility cultivation is still a blank

Method used

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  • A cultivation method for promoting growth of transgenic tomato and accumulation of astaxanthin
  • A cultivation method for promoting growth of transgenic tomato and accumulation of astaxanthin
  • A cultivation method for promoting growth of transgenic tomato and accumulation of astaxanthin

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Embodiment 1

[0013] 1. Materials and methods:

[0014] 1.1 Research materials:

[0015] In June 2013, the astaxanthin transgenic tomato was sown (Huang Junchao et al. (HuangJC et al., 2013) screened and separated green algae through genetic engineering to obtain a ketosis enzyme that can catalyze β-carotene and zeaxanthin into astaxanthin in plant cells. and hydroxylase gene into wild-type tomato plants (cv.UC82B), and the obtained transgenic tomato (has been preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee on May 14, 2014, and the preservation number is: CGMCCNo9223 , Classified and named: Xiahong No. 1 transgenic tomato.) 120 seedlings were transplanted in the cultivation greenhouse of Kunming Institute of Botany two months after emergence (Alt.

[0016] 1.2 Research methods:

[0017] 1.2.1 Experimental design: The transgenic tomato seedlings were shaded with nylon nets respectively, and the light intensity was measured by a...

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Abstract

A cultivation method for promoting the growth of transgenic tomato and the accumulation of astaxanthin, using astaxanthin transgenic tomato as material, sowing transgenic tomato seeds, transplanting the seedlings two months after emergence in a cultivation greenhouse, and covering the transgenic tomato seedlings with nylon nets respectively Shade treatment, using a quantum illuminance meter to measure the light intensity, respectively shading 75%, 50%, 30% and full light four light gradients for treatment, the photosynthesis was measured at the flowering and fruiting stages, and harvested three times when the fruit was ripe , to determine the content of astaxanthin in the fruit; during the planting period, water regularly to ensure a good water state, and use fans and wet curtains to cool down, and regularly eliminate insects and prevent diseases. The present invention uses astaxanthin transgenic tomato as a material for efficient production of astaxanthin in a cell factory. Under the condition that the light intensity is 30% of sunshine, the photosynthetic ability of the transgenic tomato and the content of astaxanthin in the fruit are the best. The technical system of artificial high-efficiency planting of transgenic tomato has laid the foundation.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a cultivation method for promoting the growth of transgenic tomato and the accumulation of astaxanthin. Background technique [0002] The carotenoids (such as β-carotene, lutein and zeaxanthin) that photosynthetic organisms participate in photosynthesis are non-ketone carotenoids, which are the only source of vitamin A synthesized by the human body. Some unicellular green algae can also synthesize and accumulate ketocarotenoids, such as astaxanthin, under stress. Astaxanthin's unique molecular structure and strong antioxidant activity endow it with high-efficiency anti-radiation, anti-aging, anti-tumor and prevention of cardiovascular and other diseases. This makes astaxanthin a new favorite in industries such as health and beauty. However, the sources of natural astaxanthin are very limited, and humans generally obtain astaxanthin from eating seafood such as shrimp, cr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G1/00
Inventor 常玮王华胡虹严宁黄俊潮
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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