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III type turkey herpes virus freeze-drying vaccine

A turkey herpes virus and vaccine technology, applied in the field of type III turkey herpes virus vaccine, can solve the problems of vaccine production and quality limitations, difficult environmental conditions for cultivation, limited cell growth area, etc., and achieve stable immune effect and production equipment Effect of small size and increased rate of infected cells

Active Publication Date: 2013-06-05
PU LIKE BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the main HVT vaccines on the market are basically obtained by using spinner flasks or shake flasks. The above-mentioned methods are labor-intensive, occupy a large space, and are easily polluted during the operation; the cell growth area provided per unit volume is limited, and the cell density is low; The environmental conditions are difficult to detect and control, and the batch-to-batch variation is large, which greatly limits the production and quality of vaccines.

Method used

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  • III type turkey herpes virus freeze-drying vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 Preparation technology of turkey herpes virus vaccine

[0035] The main steps of the preparation technology for preparing the turkey herpes virus freeze-dried live vaccine are as follows:

[0036] (1) Preparation of chicken embryo fibroblast (CEF) monolayer:

[0037] Select 10-day-old well-developed SPF chicken embryos, first use iodine tincture cotton balls to sterilize the air chamber, and then use 75% alcohol cotton balls to deiodine, aseptically pick out the embryos and place them in a sterile dish, and use 0.1M PBS. Wash the embryos and remove the eyes, claws and internal organs, then prepare a CEF suspension by continuous digestion to make a cell density of 800,000-1.2 million / ml, and inoculate the cell factory after mixing, 150-250ml per layer, 4% serum concentration M199 growth solution, cover the liquid inlet cover, place it in a 37°C incubator for 24-36 hours, and grow into a cell monolayer for later use;

[0038] (2) Preparation of working virus ...

Embodiment 2

[0051] Embodiment 2 Comparison of immunization effects of turkey herpes virus

[0052] Design experiment, comparatively utilize the qualified low-generation turkey herpes virus live vaccine of the preparation of embodiment 1 and the chicken Marek's disease turkey herpes virus live vaccine that provides with reference to the 2000 edition " Regulations of Veterinary Biological Products of the People's Republic of China " to manufacture And the immune effect of the turkey herpes virus live vaccine produced by the test procedure.

[0053] (1) Prepare seedlings according to the method of biological product regulations

[0054] Prepare the working virus seed according to the method of Example 1, then inoculate the spinner bottle, harvest the cells, freeze-dry after lyzing, and can be used after passing the test.

[0055] (2) immunity

[0056] 100 1-day-old SPF chickens provided by Beijing Merial Verton Experimental Technology Co., Ltd. were randomly divided into 4 groups, 20 in ea...

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Abstract

The invention belongs to the technical field of biopharming, and discloses a method for preparing a chicken Marek's disease turkey herpes virus Fc126 strain live vaccine by utilizing a cell factory and the turkey herpes virus vaccine (Fc126 strain) prepared by utilizing the method. The turkey herpes virus vaccine (Fc126 strain) prepared in the cell factory is good in quality, high in immunization potency and stable in immunization effect. In addition, the turkey herpes virus vaccine (Fc126 strain) has the advantages of being low in generation, high in content of plaque forming unit (PFU), low in cost, simple in operation technology, easy to control and the like.

Description

technical field [0001] The invention relates to a type III turkey herpes virus vaccine, in particular to a type III turkey herpes virus freeze-dried vaccine. Background technique [0002] Type III turkey herpesvirus (HVT) vaccine is an avirulent strain isolated from turkey flocks by American scholar Dr. Writter RL. In the chicken body of this vaccine, HVT in the cells can make the body produce antibodies, but cannot neutralize MDV. The vaccine has an interfering effect. The wide application of the vaccine can effectively resist virulent. It can exist in a cell-bound manner, and a small amount can be freed from extracellular. Its advantage is that it can be made into freeze-dried vaccines that are easy to store and transport. Since the 1970s, HVT vaccines have played a huge role. [0003] Considering that the HVT vaccine is convenient to store, transport and use, and the cost is low, the HVT vaccine is still used to prevent the outbreak of MDV in areas with good hygiene c...

Claims

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Application Information

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IPC IPC(8): A61K39/245A61K9/19A61P31/22
Inventor 张许科孙进忠白朝勇
Owner PU LIKE BIO ENG
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