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Application of sanguinarine in the preparation of antifungal biofilm drugs

A biofilm, sanguinarine technology, applied in the field of medicinal chemistry, can solve the problems of adverse reactions of receptors, unsuitable for antifungal biofilms, and no inhibition of biofilms.

Inactive Publication Date: 2016-02-03
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, fluconazole, the most widely used clinical antifungal drug, has no inhibitory effect on biofilms at all.
In addition, amphotericin B, which has the strongest antifungal effect at present, has a certain inhibitory effect on biofilm only at high concentrations, but at this concentration it will cause serious adverse reactions to the receptors, so it is not suitable for clinical application in antifungal applications. biofilm

Method used

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  • Application of sanguinarine in the preparation of antifungal biofilm drugs
  • Application of sanguinarine in the preparation of antifungal biofilm drugs
  • Application of sanguinarine in the preparation of antifungal biofilm drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] Example 1: Preparation of antifungal biofilms

[0078] Take 4g PVA, 0.7g sorbic acid, 0.5g glycerin and add 10ml water and mix well, after soaking and swelling, heat to 90 degrees Celsius to dissolve, add sanguinarine ground into micropowder, add 20ml water, stir well, keep at 45 degrees Celsius set to remove air bubbles. After preheating the glass plate to the same temperature, coat the film to a thickness of about 0.15mm, dry at 70 degrees Celsius, and release from the mold.

Embodiment 2

[0079] Example 2: Preparation of Antifungal Biofilm Emulsion

[0080] Take 8ml of distilled water and put it in a beaker, add 4g of rubber powder to make a mortar, transfer the mortar to a mortar and add 12g of sanguinarine in batches, add and grind until colostrum is formed, and then divide the colostrum with a small amount of distilled water. Transfer to the measuring cup, add the ethyl paraben alcohol solution dropwise with stirring, and finally add distilled water to the full amount, and stir well.

Embodiment 3

[0081] Example 3: Preparation of Antifungal Biofilm Ointment

[0082] Take the raw drug and grind it into micropowder, add oil phase, water phase, emulsifier and preservative matrix respectively according to a certain proportion, stir and mix in a heating water bath, cool to room temperature and stir again to make sanguinarine and matrix completely mixed to obtain an ointment.

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Abstract

The invention relates to the application of sanguinarine and pharmaceutically acceptable salts thereof in the preparation of antifungal biofilm drugs, medical equipment or medical materials, the fungus is Candida, Cryptococcus neoformans or Aspergillus, the antifungal Biofilm is to inhibit the growth and proliferation / formation of fungal biofilm or to inhibit / destroy the formed fungal biofilm. The present invention also relates to methods for non-therapeutic inhibition of fungal biofilms in vitro using sanguinarine. The compound sanguinarine used in the present invention can effectively inhibit the growth of fungal film even at a low concentration, or inhibit the mature biofilm, and the minimum effective application concentration is only 1.25? μmol / L, can be used in clinical antifungal biofilm.

Description

technical field [0001] The invention relates to the technical field of medicinal chemistry, in particular to the application of sanguinarine in the preparation of antifungal biofilm drugs. Background technique [0002] Sanguinarine (Sanguinarine) is an alkaloid present in plants such as Boluohui and Celandine. Its structural formula is shown in (I), and its molecular formula is C 20 h 14 NO 4 + , molecular weight 332. [0003] (I) [0004] Sanguinarine has rich medicinal value, can inhibit a variety of pathogenic bacteria, and has anti-tumor, insecticidal and other activities. Kunming General Hospital Natural Medicine Research Center MengF et al. have reports on sanguinarine against human pathogenic fungi (MengF, ZuoG, HaoX, WangG, XiaoH, ZhangJ, XuG.Antifungal activity of the benzo[c]phenanthridinealkaloids from ChelidoniummajusLinnaagainstresistant clinicalyeast isolates.JEthnopharmacol.2009;12(3009); :494-600.) showed that the minimum inhibitory concentration of s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N43/90A01P3/00A61L31/16A61L15/44A61L29/16
Inventor 王彦胡淦海胡丹丹张卓尔毕爽曹颖瑛姜远英
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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