High-density culture method for improving chlorophyll and protein content of chlorella at same time

A protein content, high-density culture technology, applied in the field of microalgae biology, to achieve the effect of increasing protein content and chlorophyll content, shortening the adaptation period

Inactive Publication Date: 2014-11-19
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no relevant report on how to improve the cell components of heterotrophically produced Chlorell

Method used

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  • High-density culture method for improving chlorophyll and protein content of chlorella at same time
  • High-density culture method for improving chlorophyll and protein content of chlorella at same time
  • High-density culture method for improving chlorophyll and protein content of chlorella at same time

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Cultivate Chlorella pyrenoidosa as follows:

[0040] (1) Inoculate the activated Chlorella pyrenoidosa in a 250 ml Erlenmeyer flask filled with 100 ml of Basal medium (containing 10 g / l glucose), and continuously Light culture for 6 days, so that it is in the logarithmic growth phase;

[0041] (2) Insert the seed solution obtained in step (1) into a 250ml Erlenmeyer flask containing 50 ml of medium by 8% (v / v) inoculum size, and the initial biomass concentration is 0.25 ± 0.05g / l. Among them, the formula of the culture medium is: KH 2 PO 4 1250 mg / L, MgSO 4 ·7H 2 O 1000 mg / L, EDTA-2Na 500 mg / L, H 3 BO 3 114.2mg / L, CaCl 2 2H 2 O 111mg / L, FeSO 4 ·7H 2 O 49.8 mg / L, ZnSO 4 ·7H 2 O 88.2 mg / L, MnCl 2 4H 2 O 14.2mg / L, MoO 3 7.1mg / L, CuSO 4 ·5H 2 O 15.7 mg / L, (CoNO 3 ) 2 ·6H 2 O 4.9 mg / L, add glucose and sodium nitrate in Basal medium simultaneously, the glucose addition amount is 50g / L (the molar concentration of carbon element in the medium is about 0...

Embodiment 2～4

[0045] Embodiments 2~4 are all basically the same as Example 1, the difference is that the carbon-nitrogen ratio of the culture medium used in step (2) is different, and the carbon-nitrogen ratio of the culture medium used in step (2) is respectively 56.7 in the embodiment 2~4 , 37.8, 28.4.

[0046] Embodiment 1~4 culture effect analysis:

[0047] Depend on figure 1 As shown, when the glucose concentration is 50g / l and the carbon-nitrogen ratio of the medium is in the range of 28.4-113.4, the maximum biomass concentration of Chlorella pyrenoidosa increases with the decrease of the carbon-nitrogen ratio. The maximum biomass concentrations obtained in Examples 1 to 4 were 13.64g / l, 20.01g / l, 20.31g / l, and 21.45g / l respectively, and the maximum biomass concentration obtained in Example 4 was the highest. Depend on figure 2 and image 3It can be seen that on the first day after the seed solution was inoculated in the medium for heterotrophic culture, Chlorella pyrenoidosa ch...

Embodiment 5

[0049] Cultivate Chlorella pyrenoidosa as follows:

[0050] (1) Inoculate the activated Chlorella pyrenoidosa in a 250ml Erlenmeyer flask filled with 100ml Basal medium (containing 10g / l glucose), and continuously Light culture for 6 days, so that it is in the logarithmic growth phase;

[0051] (2) the seed liquid obtained in step (1) is inserted in the 250ml Erlenmeyer flask of 50ml by the inoculum amount of 8% (v / v), and the initial biomass concentration is 0.30 ± 0.05g / l . Among them, the formula of the culture medium is: KH 2 PO 4 1250 mg / L, MgSO 4 ·7H 2 O 1000 mg / L, EDTA-2Na 500 mg / L, H 3 BO 3 114.2mg / L, CaCl 2 2H 2 O 111mg / L, FeSO 4 ·7H 2 O 49.8 mg / L, ZnSO 4 ·7H 2 O 88.2 mg / L, MnCl 2 4H 2 O 14.2mg / L, MoO 3 7.1mg / L, CuSO 4 ·5H 2 O 15.7 mg / L, (CoNO 3 ) 2 ·6H 2 O 4.9 mg / L, add glucose and sodium nitrate in the Basal medium at the same time, the glucose addition is 50 g / L, the addition of sodium nitrate makes the carbon-nitrogen ratio in the medium be...

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PUM

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Abstract

The invention provides a high-density culture method. The high-density culture method comprises the following steps: (1) activated chlorella is cultured with successive illumination to enable the chlorella to be in logarithmic phase; (2) the culture solution of the chlorella which is in the logarithmic phase in the step (1) is used as a seed solution and collected in a heterotrophic fermentation container for heterotrophic culture in darkness with the culture temperature of 28-35 DEG C and the culture time of 3-5 days, the carbon nitrogen ratio of a culture medium used in the heterotrophic fermentation container is 28.4-113.4; (3) the culture solution of the chlorella is diluted until the cell density of the chlorella reaches 10-20 g/L, a nitrogen source is added into the culture solution of the chlorella to enable the culture solution of the chlorella to contain 14.7-88.2 mol/L of nitrogen, the chlorella is conducted with photoinduction culture with the culture temperature of 20-35 DEG C and the culture time of 1-3 days. According to the invention, the culture method can improve the chlorophyll and protein content of the chlorella at the same time.

Description

technical field [0001] The invention relates to a method for cultivating chlorella, belonging to the field of microalgae biotechnology, in particular to an induced high-density cultivation method for simultaneously increasing the contents of chlorophyll and protein in chlorella. Background technique [0002] Chlorella is a single-celled green algae of the genus Chlorella. Chlorella has fast growth, high protein content, and complete amino acid components. It can provide essential amino acids for human and animal growth. It is used as a healthy food after large-scale cultivation Or aquaculture bait. As a new protein resource, Chlorella has not found any adverse reactions in toxicity testing and animal experiments. Chlorella contains pigments, minerals, vitamins and trace elements, and is a high-value protein resource that is comparable to or even superior to traditional protein resources. The content of microalgae protein is the main determinant of microalgae nutritional va...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12R1/89
Inventor 魏东陈娇敏
Owner SOUTH CHINA UNIV OF TECH
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