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Application of wild horse chasing sesquiterpene parts in the preparation of anti-acute lung injury drugs

A technology for acute lung injury and sesquiterpene, which is applied to the application field of wild horse sesquiterpene parts in the preparation of anti-acute lung injury medicines

Active Publication Date: 2017-07-07
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on whether these five sesquiterpene compounds have anti-acute lung injury effects

Method used

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  • Application of wild horse chasing sesquiterpene parts in the preparation of anti-acute lung injury drugs
  • Application of wild horse chasing sesquiterpene parts in the preparation of anti-acute lung injury drugs
  • Application of wild horse chasing sesquiterpene parts in the preparation of anti-acute lung injury drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Preparation of Sesquiterpene Fraction from Yemazhui and Detection of Active Components

[0044] (1) Preparation of the sesquiterpene part of Yemazhui:

[0045] Sample 1: Weigh 300g of Yemazhui dried whole herb, cut into 2-3cm sections, add 10 times the volume of 80% ethanol aqueous solution, reflux extraction for 2 hours, repeat twice, recover the solvent under reduced pressure at 40°C to obtain Yemazhui Ethanol extract 45g; Dissolve this ethanol extract in about 90ml of water, absorb through 500ml of D101 macroporous adsorption resin, remove macromolecular compound with 5000ml water elution first, be 95% ethanol aqueous solution elution with 5000ml volume fraction behind, reduce The solvent was recovered under pressure to obtain 17g of macroporous resin; the obtained macroporous resin was adsorbed by 350ml of YMCODS-A reversed-phase silica gel filler particle size 60um reversed-phase silica gel column, eluted with 1750ml volume fraction of 50% methanol aqueous solution...

Embodiment 2

[0058] Anti-acute lung injury experiment of Yemazhui sesquiterpene fraction (EUP-SQT) in mice

[0059] 1. Experimental drugs and reagents: 0.5% sodium carboxymethylcellulose; lipopolysaccharide (LPS), Sigma-Aldrich, USA; dexamethasone acetate tablets, Zhejiang Xianju Pharmaceutical Co., Ltd.; polyclonal rabbit anti-human C3c complement, Shanghai Ruiqi Biotechnology Co., Ltd.; distilled water; normal saline; 20% urethane-normal saline; 4% formalin, H-E staining solution, 3,3'-diaminobenzidine (DAB) chromogenic solution, etc.

[0060] 2. Experimental mice: Balb / c mice, male, weighing 24-28 grams, provided by the Experimental Animal Center of Soochow University, experimental animal production license: XCYK (Su) 2002-0008. The experimental animals were raised in an environment with a temperature of 24±1° C. and a relative humidity of 40% to 80%, with free access to food and water. Before the experiment, they were adaptively fed for 7 days.

[0061] 3. Other materials: mouse tumo...

Embodiment 3

[0081] Comparative experiment on anti-acute lung injury effect of Yemazhui sesquiterpene part and Yemazhui ethanol extract

[0082] Preparation of Yemazhui ethanol extract: Weigh the dried whole herb of Yemazhui, cut it into sections of 2-3 cm, add 10 times the volume of 80% ethanol aqueous solution, reflux extraction for 2 hours, repeat twice, recover the solvent under reduced pressure, and obtain Yemazhui ethanol Extract (EtOH).

[0083] In this comparative experiment, the experimental reagents, experimental mice and experimental materials are the same as those in Example 2.

[0084] Experimental mice were randomly divided into 5 groups including blank group, model group, EtOH group of ethanol extract of Yemazhui, EUP-SQT group of sesquiterpene part of Yemazhui and positive group, 10 mice in each group. Yemazhui ethanol extract EtOH group was given EtOH at a dose of 30 mg·kg -1 ; Yemazhui sesquiterpene part SQT group was given EUP-SQT, the dose was 30mg·kg -1 ; The positi...

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Abstract

The invention discloses an application of the sesquiterpene part of Yemachai in the preparation of anti-acute lung injury medicine. In the present invention, the sesquiterpene part of Yemachai is prepared into animal medicine, and the way of administering it to mice is adopted, and lipopolysaccharide LPS is used to induce acute lung injury in mice, and then the lung wet / dry weight ratio of acute lung injury mice is measured. Nitric oxide (NO) and protein content in alveolar lavage fluid (BALF); detection of myeloperoxidase (MPO) and superoxide dismutase (SOD) activities in lung tissue homogenate; tumor necrosis factor in alveolar lavage fluid The contents of ‑α (TN‑α), interleukin‑6 (IL‑6) and interleukin‑1β (IL‑1β); the contents of complement C3 and C3c in serum; the pathological changes of mouse lung tissue after H&E staining. The results showed that the sesquiterpene fractions containing yomalide F, yomalide G, yomalide H, yomalide I and yomalide K had anti-acute lung injury activity.

Description

technical field [0001] The invention relates to the application of sesquiterpene parts of Yemachai in the preparation of anti-acute lung injury medicine. Background technique [0002] Acute lung injury (ALI) is based on diffuse lung cell injury, pulmonary edema and pulmonary inflammatory cell infiltration caused by pulmonary vascular injury are its pathological features, and the main clinical manifestation is severe hypoxemia , diffuse pulmonary infiltration and pulmonary edema; some patients will eventually develop acute respiratory distress syndrome (ARDS), resulting in irreversible acute respiratory failure and multiple organ dysfunction, with a mortality rate as high as 30-40%; The pathogenesis of ALI is complex, and the risk factors for ALI can be direct damage from the lung, or indirect damage to the lung from extrapulmonary factors through systemic inflammatory response. (See: Baffert F, Le T, Thurston G, et al. Angiopoietin-1 decreases plasma leakage by reducing num...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/365A61K36/28A61P11/00
Inventor 张健褚纯隽任慧玲陈道峰严彪李显伦吴天威徐乃玉
Owner SUZHOU UNIV
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