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Rapid propagation method of cinnamomum wilsonii gamble

A kind of Sichuan-Guizhou, fast technology, applied in the field of plants, to achieve the effect of being conducive to large-scale planting, less energy consumption, and short cycle

Inactive Publication Date: 2014-12-03
NANJING BIAOKE BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the cultivation of Osmanthus chuanxiong. Tissue culture technology is one of the effective methods for the rapid propagation of osmanthus osmanthus, which can effectively improve the resource development and full utilization of osmanthus chuanxiong.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Take the bud stems of Osmanthus cinnamon, soak in bleach for 3 minutes, remove surface stains with a brush, rinse with running water for 1.5 hours, treat with 75% alcohol on an ultra-clean workbench for 30 seconds, treat with sodium hypochlorite for 12 minutes, rinse with sterile water 5 times, and sterilize The treated stem segment with buds was inserted into the culture medium formula: WPM+IAA0.05mg / L+2ip0.5mg / l+kanamycin 15mg / L for bud induction culture, additional sucrose 30g / L, agar 6.5 g / L, PH5.8, light 3000lx, put the induced buds into the proliferation medium WPM+2,4,5-T0.05mg / L+KT0.2mg / L+1mg / LABA, add sucrose 30g / L, Agar 6.5g / L, PH5.8, light 3000lx, put the clustered buds cultured by proliferation into the rooting medium 1 / 2MS+NAA0.1mg / L+IBA0.2mg / L+250mg / L yeast extract, after rooting Chuangui test-tube seedlings were hardened and cultivated, and the survival rate of Chuangui was 90%.

Embodiment 2

[0011] Take the bud stems of Osmanthus cinnamon, soak in bleach for 3 minutes, remove surface stains with a brush, rinse with running water for 1.5 hours, treat with 75% alcohol on an ultra-clean workbench for 30 seconds, treat with sodium hypochlorite for 12 minutes, rinse with sterile water 5 times, and sterilize The treated stem segment with buds was inserted into the culture medium formula: WPM+IAA0.2mg / L+2ip1mg / l+kanamycin 15mg / L induction medium for bud induction culture, additional sucrose 30g / L, agar 6.5g / L L, PH5.8, light 3000lx, put the induced buds into the proliferation medium WPM+2,4,5-T0.15mg / L+KT0.4mg / L+1mg / LABA, add sucrose 30g / L, agar 6.5 g / L, PH5.8, light 3000lx, put the clustered buds cultured by proliferation into the rooting medium 1 / 2MS+NAA0.1mg / L+IBA0.2mg / L+250mg / L yeast extract, after rooting, Chuangui The test-tube seedlings were hardened and cultivated, and the survival rate of Chuangui was 91%.

Embodiment 3

[0013] Take the bud stems of Osmanthus cinnamon, soak in bleach for 3 minutes, remove surface stains with a brush, rinse with running water for 1.5 hours, treat with 75% alcohol on an ultra-clean workbench for 30 seconds, treat with sodium hypochlorite for 12 minutes, rinse with sterile water 5 times, and sterilize The treated stem segment with buds is inserted into the culture medium with WPM+IAA0.1mg / L+2ip0.5mg / l+kanamycin 15mg / L for bud induction culture, additional sucrose 30g / L, agar 6.5 g / L, PH5.8, light 3000lx, put the induced buds into the proliferation medium WPM+2,4,5-T0.1mg / L+KT0.2mg / L+1mg / LABA, add sucrose 30g / L, Agar 6.5g / L, PH5.8, light 3000lx, put the clustered buds cultured by proliferation into the rooting medium 1 / 2MS+NAA0.1mg / L+IBA0.2mg / L+250mg / L yeast extract, after rooting Chuangui test-tube seedlings were hardened and cultivated, and the survival rate of Chuangui was 92%.

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PUM

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Abstract

The invention relates to a rapid propagation method of cinnamomum wilsonii gamble. The rapid propagation method comprises the steps of explant disinfection, axillary bud induction, cluster bud propagation, rooting induction and hardened-seedling transplantation. The tissue culture method of cinnamomum wilsonii gamble has the advantages of simplicity and convenience in operation, short growth cycle, high propagation coefficient and high survival rate, and a large quantity of cinnamomum wilsonii gamble seedlings can be obtained within a short period of time, so that large-scale planting is realized.

Description

technical field [0001] The invention relates to a rapid propagation method for tissue culture of osmanthus chuanxiong, belonging to the technical field of plants. Background technique [0002] Chuan Gui, Cinnamomum wilsonii Gamble , Arbor, Lauraceae, produced in Shaanxi, Sichuan, Hubei, Hunan, Guangxi, Guangdong and Jiangxi. Born in valleys or hillsides in the sun or along the ditch, in sparse forests or dense forests, at an altitude (30-300) 800-2400 meters. The branches, leaves and fruits contain aromatic oil, and the oil is used as a raw material for blending food or soap essence. The bark of Osmanthus chuanxiong is used as medicine to invigorate the kidney, dispel cold and dispel wind, and treat rheumatism, muscle and bone pain, bruises, abdominal pain, vomiting and diarrhea. Sichuan cinnamon was used as a spice in ancient times in the West. It is used in Chinese cuisine to flavor stews and is an ingredient in five-spice powder. At present, there is no report on the...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 张金芳杨存
Owner NANJING BIAOKE BIO TECH
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