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Bletilla striata initial tissue culture method

A Bletilla striata, tissue culture technology, applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve the problems of lack of wild Bletilla striata resources, low rooting and germination rate of Bletilla striata seeds, difficult application, etc., and achieves strong promotion value and composition. Simple, well-proportioned results

Inactive Publication Date: 2018-12-07
贵州发来地农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Because bletilla striata has great utilization value in the medicinal field, but wild bletilla striata resources are scarce, and the rooting and germination rate of bletilla striata seeds is low, it is difficult to make it widely used. Therefore, how to improve the rooting and germination rate of bletilla striata and increase the output of bletilla striata Very important

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: A method for primary tissue culture of Bletilla striata, including the following steps:

[0022] (1) Seed disinfection: Use 55% alcohol to soak and disinfect the seeds for 15 minutes, and filter out the seeds for later use;

[0023] (2) Preparation of culture medium: sawn paulownia wood, sucrose, banana puree, agar powder, perilla stem, Weilingxian, Leulu, Qianghuo ammonium nitrate, calcium chloride dihydrate, magnesium sulfate heptahydrate, potassium dihydrogen phosphate , Benzyl purine, naphthalene acetic acid, zinc sulfate heptahydrate, sodium molybdate, cobalt chloride, ferrous sulfate, inositol, nicotinic acid, glycine, put it into a culture flask and sterilize it in an autoclave. The culture medium is subdivided into petri dishes to obtain culture medium;

[0024] (3) Induce germination: evenly spread the seeds into a petri dish with medium and add 0.02g / l gibberellin to induce seed germination;

[0025] (4) Aseptic culture: Move the germinated Bletilla striat...

Embodiment 2

[0029] Embodiment 2: A method for primary tissue culture of Bletilla striata, including the following steps:

[0030] (1) Seed disinfection: Use 50% alcohol to soak and disinfect the seeds for 20 minutes, and filter out the seeds for later use;

[0031] (2) Preparation of culture medium: sawn paulownia wood, sucrose, banana puree, agar powder, perilla stem, Weilingxian, Leulu, Qianghuo ammonium nitrate, calcium chloride dihydrate, magnesium sulfate heptahydrate, potassium dihydrogen phosphate , Benzyl purine, naphthalene acetic acid, zinc sulfate heptahydrate, sodium molybdate, cobalt chloride, ferrous sulfate, inositol, nicotinic acid, glycine, put it into a culture flask and sterilize it in an autoclave. The culture medium is subdivided into petri dishes to obtain culture medium;

[0032] (3) Induce germination: evenly spread the seeds into a petri dish with medium and add 0.02g / l gibberellin to induce seed germination;

[0033] (4) Aseptic culture: Move the germinated Bletilla str...

Embodiment 3

[0037] Embodiment 3: A method for the first generation tissue culture of Bletilla striata, including the following steps:

[0038] (1) Seed disinfection: Use 60% alcohol to soak and disinfect the seeds for 10 minutes, and filter out the seeds for later use;

[0039] (2) Preparation of culture medium: sawn paulownia wood, sucrose, banana puree, agar powder, perilla stem, Weilingxian, Leulu, Qianghuo ammonium nitrate, calcium chloride dihydrate, magnesium sulfate heptahydrate, potassium dihydrogen phosphate , Benzyl purine, naphthalene acetic acid, zinc sulfate heptahydrate, sodium molybdate, cobalt chloride, ferrous sulfate, inositol, nicotinic acid, glycine, put it into a culture flask and sterilize it in an autoclave. The culture medium is subdivided into petri dishes to obtain culture medium;

[0040] (3) Induce germination: evenly spread the seeds into a petri dish with medium and add 0.02g / l gibberellin to induce seed germination;

[0041] (4) Aseptic culture: Move the germinated...

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PUM

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Abstract

The invention discloses a bletilla striata initial tissue culture method. The method comprises the following steps: soaking and disinfecting seeds by using alcohol, filtering out the seeds for later use; then mixing paulownia saw wood, sucrose, banana paste, agar powder, purple perilla stems, radix clematidis, uniflower swisscentaury root, notopterygium root, ammonium nitrate, calcium chloride dehydrate, magnesium sulfate heptahydrate, monopotassium phosphate, benzyl purine, naphthylacetic acid, zinc sulfate heptahydrate, sodium molybdate, cobalt chloride, ferrous sulfate, inositol, niacin andglycine, putting the mixture into a culture bottle, and sterilizing by using a high-pressure sterilization pot, subdividing the sterilized culture medium into culture dishes to obtain the culture medium; uniformly spreading the seeds into the culture dishes filled with the culture medium, adding gibberellin; moving the sprouted bletilla striata shoots in the culture dishes into a sterile cultureroom and culturing for 30-40 days. The method solves the technical problem, and has the characteristics of promoting rooting and sprouting of bletilla striata and improving the yield of bletilla striata.

Description

Technical field [0001] The invention relates to a method for tissue culture of Bletilla striata, in particular to a method for tissue culture of Bletilla striata primary generation. Background technique [0002] Bletilla striata, (scientific name: Bletilla striata), also known as Lianhecao, Gangen, Baigui, Rulan, Zhulan, Zilan, Purple Crotch, Baili, Ground Screw, White Chicken Baby, White Root, Lamb's Horn Seven. Perennial herbaceous bulbous plants (tuberous roots), the plant height is 18-60 cm. Mainly distributed in China, Japan and northern Myanmar. The main flowering period is spring, but depending on the climate of each region, it may bloom from late winter to early summer. Bletilla has a wide range of medicinal and garden values. Mainly used for astringent to stop bleeding, reduce swelling and build muscle. The flowers are purple, white, blue, yellow and pink. They can be viewed indoors in potted plants, and can also be dotted in shaded flower stands, flower borders or c...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 邬林
Owner 贵州发来地农业科技有限公司
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