Check patentability & draft patents in minutes with Patsnap Eureka AI!

Method for extracting robinin from robinia pseudoacacia flowers

A technology of locust glycoside and locust flower, which is applied in the field of ultrasonic-microwave collaborative extraction of locust glucoside, which can solve the problems of difficult large-scale production, large amount of ethanol, and high equipment cost, and achieve low solvent toxicity, stable product quality, and low preparation cost. Effect

Inactive Publication Date: 2014-12-03
NANJING BIAOKE BIO TECH
View PDF0 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, in the document "Optimizing the Extraction Process of Robinia pseudoacacia in Robinia pseudoacacia by Response Surface Method", the report adopts the ethanol reflux extraction method, the amount of ethanol is large, and the extraction rate is low; Extraction method, high equipment cost, difficult maintenance, difficult to mass production

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Take Robinia pseudoacacia flowers, remove impurities, dry at 60°C, crush, pass through a 80-mesh sieve, take 1kg of powder and add 9 times the 60% ethanol solution, soak for 30 minutes, use ultrasonic-microwave to extract, control ultrasonic 400W (normally open ), with a microwave power of 500W, extract for 2 hours, centrifuge, take the supernatant, concentrate, dissolve the concentrate in water, and absorb it through a neutral alumina column. The particle size of the neutral alumina is 50 mesh. The mass ratio of alumina is 1:4, the height-to-diameter ratio of the column is 4:1, 10 times of 50% ethanol is used for elution, the eluate is collected, concentrated under reduced pressure, and the crude acaciaside is obtained, and then ethanol-methanol ( 1:1) mixed solvent recrystallization twice to obtain 20.8 g of acaciaside, which was detected by HPLC with a purity of 97.9%.

Embodiment 2

[0017] Take Robinia pseudoacacia flowers, remove impurities, dry at 60°C, crush, pass through a 80-mesh sieve, take 1kg of powder and add 6 times the 65% ethanol solution, soak for 50min, use ultrasonic-microwave to extract, control ultrasonic 400W (normally open ), the microwave power is 550W, extract for 2.5h, centrifuge, take the supernatant, concentrate, the concentrate is heated to dissolve in water, and adsorbed by a neutral alumina column. The particle size of the neutral alumina is 80 mesh. The mass ratio of permanent alumina is 1:5, the height-diameter ratio of the column is 7:1, 12 times of 60% ethanol is used for elution, the eluate is collected, and concentrated under reduced pressure to obtain the crude acaciaside, which is then ethanol-methanol (1:1) mixed solvent was recrystallized twice to obtain 23.1 g of acaciaside, which was detected by HPLC with a purity of 98.7%.

Embodiment 3

[0019] Take Robinia pseudoacacia flowers, remove impurities, dry at 60°C, crush, pass through a 80-mesh sieve, take 1kg of powder and add 4 times the 70% ethanol solution, soak for 60 minutes, use ultrasonic-microwave to extract, control ultrasonic 400W (normally open ), with a microwave power of 600W, extract for 3 hours, centrifuge, take the supernatant, concentrate, dissolve the concentrate in heated water, and pass through a neutral alumina column for adsorption. The mass ratio of alumina is 1:6, the height-to-diameter ratio of the column is 10:1, 15 times of 70% ethanol is used for elution, the eluate is collected, concentrated under reduced pressure to obtain the crude acaciaside, and then ethanol-methanol ( 1:1) mixed solvent recrystallization twice to obtain 21.4 g of acaciaside, which was detected by HPLC with a purity of 98.4%.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle size (mesh)aaaaaaaaaa
Login to View More

Abstract

The invention relates to a method for extracting robinin from robinia pseudoacacia flowers and in particular relates to a method for extracting robinin in an ultrasound-microwave synergistic mode. The method comprises the following steps: drying and grinding robinia pseudoacacia flowers; performing ultrasonic-microwave assisted extraction; performing alumina column chromatography, collecting the eluant, and performing vacuum concentration to obtain a crude robinin product; and performing ethanol-methanol mixing and recrystallizing. Compared with the prior art, the method disclosed by the invention has the advantages of simple process, high yield, high purity and short extraction time and is suitable for industrial production.

Description

technical field [0001] The invention relates to a method for extracting acacia glycosides from Robinia pseudoacacia flowers, in particular to a method for ultrasonic-microwave synergistic extraction of acacia glycosides. Background technique [0002] Robinia pseudoacacia is the dried flower of the leguminous plant Robinia pseudoacacia. Part of it fell to waste, and has high medicinal value. It has the functions of eliminating carbuncle and blood stasis, clearing away heat and detoxification, treating throat and hemorrhoids, strengthening stomach and clearing intestines. Locust glycosides, also known as acacia glycosides, the molecular formula is C 28 h 32 o 14 , Soluble in hot water, hot ethanol, almost insoluble in ether. Animal experiments have shown diuretic, strong, anti-inflammatory effects. It is clinically used to treat acute and chronic nephritis, uremia, cystitis and other urinary system diseases. [0003] At present, there are few techniques for extracting ac...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/07C07H1/08
Inventor 张金芳杨存
Owner NANJING BIAOKE BIO TECH
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com