Cultivation method of ganoderma lucidum casein film crystal
A technology of seed-flesh ganoderma cheese and cultivation methods, applied in botany equipment and methods, horticulture, applications, etc., can solve problems such as inconvenient eating, slow growth rate of ganoderma lucidum, limit market development potential, etc., and achieve the goal of increasing growth rate Effect
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[0035] (1) Preparation of meat ganoderma culture medium: 5-20g ginseng, 5-25g ganoderma, 10-100g fungus, 10-50g wolfberry, 5-30g white fungus, 10-50g malt, 10-50g rice, 10-50g sugar , 5-30g of honey and 1000-2000ml of drinking water are put into a high-speed homogenizer and crushed into a slurry, then steamed for 20-90 minutes, filtered to remove slag, and the culture medium of Ganoderma lucidum is obtained.
[0036] (2) Put the ganoderma lucidum strain prepared in step 1 into 1000-2000ml of ganoderma lucidum culture solution with pH 5-6, cultivate ganoderma lucidum at a temperature of 20-40°C and a humidity of 60-70%, and use 0.5mol / The LNaOH solution and the dilute hydrochloric acid solution adjusted the ganoderma lucidum culture solution to keep the pH at 5-6 all the time, and obtained casein crystals on the surface of the ganoderma lucidum culture solution after 3-7 weeks.
[0037] 3. Cultivation of Ganoderma lucidum cheese film crystals:
[0038] (1) Preparation of Gano...
Embodiment 1
[0042]1. Obtaining the meat ganoderma: cut off the surface layer of the meat ganoderma, cut into meat pieces of 9 cm in length, width, and 3 cm in height, rinse the meat ganoderma with medical alcohol for 5 minutes, and obtain the meat ganoderma strain. The block is about 20g.
[0043] 2. Obtaining of Ganoderma lucidum cheese film crystals:
[0044] (1) Preparation of Ganoderma lucidum culture medium: put 20g ginseng, 100g fungus, 50g wolfberry, 30g white fungus, 50g malt, 150g rice, 50g sugar, 30g honey and 1000ml drinking water, put them into a high-speed homogenizer and crush them into a pulp , and then steamed for 90 minutes, filtered to remove the residue, and obtained the culture solution of Ganoderma lucidum.
[0045] (2) Put the ganoderma lucidum bacterial classification into 1000ml ganoderma lucidum culture fluid, and use 0.5mol / ml NaOH solution and dilute hydrochloric acid solution to adjust the culture medium to keep at pH 5 all the time, cultivate ganoderma lucidu...
Embodiment 2
[0050] 1. Obtaining the ganoderma lucidum: cut off the surface layer of the ganoderma lucidum, cut into meat pieces of 9 cm in length, width, and 3 cm in height, rinse and disinfect the ganoderma meat pieces with medical alcohol for 9 minutes, and obtain the ganoderma lucidum strains.
[0051] 2. Obtaining of Ganoderma lucidum cheese film crystals:
[0052] (1) Preparation of Ganoderma lucidum culture medium: Put 5g ginseng, 10g fungus, 10g wolfberry, 5g white fungus, 10g malt, 10g rice, 10g sugar, 5g honey and 2000ml drinking water, put them into a high-speed homogenizer and crush them into a pulp , and then steamed for 20 minutes, filtered to remove the residue, and obtained the culture solution of Ganoderma lucidum.
[0053] (2) Put the strains of Ganoderma lucidum into 2000ml of Ganoderma lucidum culture medium with pH 5-6, cultivate Ganoderma lucidum under the environmental conditions of temperature 20°C and humidity 60%, and adjust the cultivation of Ganoderma lucidum wi...
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