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Method for simultaneous determination of cyclic adenosine monophosphate, baicalin and glycyrrhizic acid in Xiaochaihu granules

A technology of cyclic adenosine monophosphate and Xiaochaihu granules, which is applied to the high-performance liquid chromatography determination method of baicalin and glycyrrhizic acid content, in the field of cyclic adenosine monophosphate, can solve problems such as the research on the determination of cyclic adenosine monophosphate content that has not been seen, Achieve good reproducibility, high accuracy and high sensitivity

Inactive Publication Date: 2016-06-15
NINGXIA UNIVERSITY
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Problems solved by technology

[0006] Xiaochaihu granule is a traditional Chinese medicine, which is mainly made of Bupleurum, Scutellaria baicalensis, licorice, jujube, ginger pinellia, Codonopsis pilosula and ginger. Evaluation indicators, in addition to this, the current research is more on the determination of glycyrrhizic acid and saikosaponin a in Xiaochaihu granules, and jujube is also the main component of Xiaochaihu granules, but there is no evidence for its main component cyclic adenosine monophosphate Studies on the Assay of

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  • Method for simultaneous determination of cyclic adenosine monophosphate, baicalin and glycyrrhizic acid in Xiaochaihu granules
  • Method for simultaneous determination of cyclic adenosine monophosphate, baicalin and glycyrrhizic acid in Xiaochaihu granules
  • Method for simultaneous determination of cyclic adenosine monophosphate, baicalin and glycyrrhizic acid in Xiaochaihu granules

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Embodiment 1

[0025] 1.1 Instruments and materials:

[0026] AGILENT1100 high-performance liquid chromatography, AL204 electronic balance, 101A-1E electric blast drying oven, TDL80-2B desktop centrifuge, KQ-250DE numerical control ultrasonic cleaner, SHB-Ⅲ circulating water multi-purpose vacuum pump.

[0027] 1.2 Drugs and reagents:

[0028] Methanol, potassium dihydrogen phosphate (analytical grade), cyclic adenosine monophosphate, baicalin, glycyrrhizinic acid reference substance (China Pharmaceutical and Biological Products Identification Institute), Xiaochaihu granules (Yunnan Baiyao Group Co., Ltd.), laboratory water is ultra-pure water.

[0029] 1.3 Reference substance stock solution:

[0030] Accurately weigh 0.0020 g of cyclic adenosine monophosphate, glycyrrhizic acid, and baicalin reference substances respectively, dissolve them in 50% (V:V, the same below) methanol, transfer to a 10 mL volumetric flask, set the volume to the mark, shake well, and Prepare 0.20 mg / mL reference s...

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Abstract

The invention relates to a method for simultaneously measuring the contents of cyclic adenosine monophosphate, baicalin and glycyrrhizic acid in Xiaochaihu granules, which is characterized in that it comprises the following steps: (1) preparing a stock solution of a reference substance; (2) preparing a solution of a test substance; (3) High performance liquid chromatography determination: measure with high performance liquid chromatography; Sample introduction respectively, measure by chromatographic conditions, do calibration curve with peak area and reference substance volume, obtain cyclic adenosine monophosphate, baicalin and glycyrrhizin The regression equations are: Y=242.17X-35.103, R 2 =0.9969; Y=178.38X+97.653, R 2 =0.9998; Y=113.69X+94.29, R 2 = 0.9987. The method of the invention adopts ultrasonic extraction and RP-HPLC to simultaneously measure cyclic adenosine monophosphate, baicalin and glycyrrhizic acid in Xiaojianzhong granules, and obtains good results.

Description

technical field [0001] The invention relates to a method for simultaneously determining the contents of cyclic adenosine monophosphate, baicalin and glycyrrhizic acid in Xiaochaihu granules, which is a high performance liquid chromatography method for determining the contents of cyclic adenosine monophosphate, baicalin and glycyrrhizic acid. Background technique [0002] Xiaochaihu granule is made by extracting and processing the original prescription of Xiaochaihu Decoction. It is composed of Bupleurum, Scutellaria, Radix Glycyrrhiza, Codonopsis, Pinellia, Ginger and Jujube. Modern pharmacological studies have shown that Xiaochaihu Decoction has a wide range of medicinal effects. Intestinal function can regulate and protect, and has a wide range of clinical applications. [0003] Cyclic adenosine monophosphate (cAMP) is the main active ingredient of jujube, a derivative of nucleotides, and a protein kinase activator. It is a biologically active substance that is ubiquitous...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 郝凤霞郝洪涛
Owner NINGXIA UNIVERSITY
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