Application of bubble walnut shell extract in the preparation of antitumor drugs

A technology of soaking walnuts and extracts, which is applied in the field of medicine to achieve the effects of simple extraction process, low cost, stable ingredients and drug efficacy

Inactive Publication Date: 2018-06-15
DALI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The results of the novelty search show that in the past, there were no relevant reports on the antitumor drugs prepared with the extract of soaked walnut shells as the main drug, thus constituting the present invention.

Method used

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  • Application of bubble walnut shell extract in the preparation of antitumor drugs
  • Application of bubble walnut shell extract in the preparation of antitumor drugs
  • Application of bubble walnut shell extract in the preparation of antitumor drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1 : Cytotoxicity test of walnut shell extract on human lung cancer cells (NCI-H292, NCI-H661)

[0019] 1.1 Preparation of soaked walnut shell extract:

[0020] Pickled walnuts (Juglans sigillata) were collected from Yangbi County, Yunnan Province, and were identified as mature fruits of Juglandaceae (Juglandaceae) by Professor Xia Conglong from the Department of Pharmacognosy, Dali College. The shells of the fruits were dried in the sun for later use.

[0021] 1.1.1 Preparation of bubble walnut shell extract PHTK-DEF

[0022] Crush 1 kg of dried soaked walnut shells, add 800 ml of sodium hydroxide solution (1 mol / liter), soak overnight at room temperature, and filter. Add 700 milliliters of sodium hydroxide solution (1 mol / liter) again, stand for 2 hours, filter, and combine the filtrates. Add glacial acetic acid to the filtrate to pH=5, let stand for 1 hour, and filter. Add 1 times the amount of ethanol (95%) to the filtrate, let it stand for 1 hour, and fi...

Embodiment 2

[0043] Example 2 : Cytotoxicity test of walnut shell extract on human liver cancer cells (HepG-2, Huh-7)

[0044] 2.1 Preparation of soaked walnut shell extract:

[0045] The same method as in Example 1.1 was used to prepare PHTK-DEF and PHTK-D, extracts of foamed walnut shells.

[0046] 2.2 Cytotoxicity test:

[0047] 2.2.1 Instruments and materials

[0048] (1) Instrument: with embodiment 1.

[0049] (2) material: with embodiment 1

[0050] (3) Cell lines: human liver cancer cells HepG-2 and Huh-7, purchased from the Cell Bank of Kunming Institute of Zoology, Chinese Academy of Sciences.

[0051] 2.2.2 Method

[0052] (1) Cell inoculation: HepG-2 and Huh-7 cells were respectively cultured in DMEM medium containing 10% inactivated FBS in a 5% carbon dioxide incubator at 37°C. After trypsinization, centrifuge, discard the supernatant, and adjust the cell concentration to 1×10 with complete medium 4 per milliliter, inoculate in a 96-well plate, 150 microliters per well...

Embodiment 3

[0063] Example 3 : Effect of bubble walnut shell extract on normal liver cells (L-02)

[0064] 3.1 Purpose of the experiment:

[0065] The toxicity of the extract was evaluated through the growth effect of the walnut shell extract on normal hepatocytes (L-02 cell line).

[0066] 3.2 Preparation of soaked walnut shell extract:

[0067] The same method as in Example 1.1 was used to prepare PHTK-DEF and PHTK-D, extracts of foamed walnut shells.

[0068] 3.3 Cytotoxicity test:

[0069] 3.3.1 Instruments and materials

[0070] (1) Instrument: with embodiment 1.

[0071] (2) Material: with embodiment 1.

[0072] (3) Cell line: normal human hepatocyte L-02, purchased from the Cell Bank of Kunming Institute of Zoology, Chinese Academy of Sciences.

[0073] 3.3.2 Method

[0074] (1) Cell inoculation: L-02 cells were cultured in DMEM medium containing 10% inactivated FBS in a 37°C, 5% carbon dioxide incubator, and the above-mentioned cells in the logarithmic growth phase were d...

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Abstract

The invention discloses application of juglans sigillata shell extractive in preparation of antitumor drugs, and relates to the application of juglans sigillata shell extractive for preparing drugs for preventing and treating tumor diseases. More specifically, the invention relates to an effective part obtained by steeping juglans sigillata shells in aqueous alkali and medical application thereof for preparing the antitumor drugs. The juglans sigillata shell is the shell of ripe juglans sigillata belonging to juglans of juglandaceae, the effective part extractive has significant effect on inhibiting viability of human lung cancer cells and human hepatoma carcinoma cells, and has very small virulence to normal human hepatocytes, thus being expected to be made into the drugs for preventing and treating various tumor diseases.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular, the invention relates to the medical use of a walnut shell extract for preparing antitumor drugs. The bubble walnut shell extract is obtained by soaking the fruit shell of the bubble walnut (Juglans sigillata D.) in an alkaline solution, alcohol precipitation, solvent recovery, and drying. Medicines for diseases. Background technique [0002] In recent years, with the improvement of living standards, the change of ecological environment and the increase of life pressure, cancer has become an upward trend, among which liver cancer and lung cancer are the two most common malignant tumors in my country. Lung cancer is the most common primary malignant tumor of the lung, most of which originate from the bronchial mucosa and bronchioalveoli, so it is also called bronchial lung cancer. Lung cancer is one of the malignant tumors with the fastest increasing morbidity and mortality and t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/52A61P35/00A61K131/00
Inventor 刘熙李春艳巫秀美于冬梅李冬梅李寅珊赵昱彭友伦
Owner DALI UNIV
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