A kind of proanthocyanidin of southern jujube and its preparation method and application
A technology of proanthocyanidins and jujube jujube, applied in the fields of application, medical formula, plant raw materials, etc., can solve the problems that other biological activities of jujube bark have not been reported in literature, etc., and achieve easy acquisition of raw materials, wide application value, and simple preparation method Effect
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Embodiment 1
[0031] Embodiment 1 A kind of preparation method of proanthocyanidins of southern jujube
[0032] Weigh 25g of dried southern jujube bark powder, place it in a 1000mL conical flask, add 800mL of 50% ethanol acidified with 0.5% (V / V) acetic acid, let it stand overnight, and place it in an ultrasonic generator at 40KHz the next day Ultrasonic extraction for 30min, Buchner funnel filtration, add 600mL 50% ethanol acidified with 0.5% acetic acid to the filter residue, ultrasonic extraction at 40KHz for 30min, Buchner funnel filtration, add 600mL 50% ethanol acidified with 0.5% acetic acid to the filter residue, 40KHz Ultrasonic extraction for 30 min, Buchner funnel filtration, three filtrates were combined, and the filtrate was concentrated under reduced pressure in a rotary evaporator at 45°C to obtain a concentrated extract, which was used for macroporous resin purification.
[0033] Take 200g of new D101 macroporous resin, put it into a chromatography column, soak it with 95% e...
Embodiment 2
[0046] Example 2 The preparation method of southern jujube proanthocyanidins
[0047] Weigh 50g of dried southern jujube bark powder, put it in a 2000mL beaker, add 1500mL of 70% ethanol acidified with 1.0% HCl (V / V) acetic acid, let it stand overnight, and place it in an ultrasonic generator at 40KHz the next day Ultrasonic extraction for 30min, Buchner funnel filtration, add 1200mL 70% ethanol acidified with 1.0% HCl to the filter residue, ultrasonic extraction for 30min at 40KHz, filter with Buchner funnel, add 1000mL 70% ethanol acidified with 1.0% HCl to the filter residue, 40KHz Ultrasonic extraction for 30 min, Buchner funnel filtration, three filtrates were combined, and the filtrate was concentrated under reduced pressure in a rotary evaporator at 45°C to obtain a concentrated extract, which was used for macroporous resin purification.
[0048] Take 400g of new X-5 macroporous resin, put it into a chromatography column, soak it with 95% ethanol for 24h, wash it with d...
Embodiment 3
[0049] Example 3 The preparation method of southern jujube proanthocyanidins
[0050] Take by weighing 50g dried Mao Mai Nan Suanzao bark coarse powder, place in a 2000mL beaker, add 1500mL of 70% methanol acidified with 1.0% HCl (V / V) acetic acid, let stand overnight, and place in an ultrasonic generator the next day, Ultrasonic extraction at 50KHz for 30min, Buchner funnel filtration, add 1200mL 70% methanol acidified with 1.0% HCl to the filter residue, ultrasonic extraction at 50KHz for 40min, Buchner funnel filtration, add 1000mL 70% methanol acidified with 1.0% HCl to the filter residue , ultrasonically extracted at 50KHz for 60min, filtered through a Buchner funnel, combined three filtrates, and concentrated the filtrate under reduced pressure in a rotary evaporator at 45°C to obtain a concentrated extract, which was used for macroporous resin purification.
[0051] Take 400g of new AB-8 macroporous resin, put it into a chromatographic column, soak it with 95% ethanol f...
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